Title:
BIOSENSOR WITH THE USE OF PIGMENT-SYNTHESIS GENE OF PURPLE NON-SULFUR BACTERIUM AND METHOD OF CONSTRUCTING THE BIOSENSOR
Document Type and Number:
WIPO Patent Application WO/2006/022235
Kind Code:
A1
Abstract:
It is intended to provide a biosensor whereby measurement can be performed by a more convenient procedure and a specific chemical can be economically and visually detected. Namely, a biosensor for detecting a specific chemical comprising a recombinant purple non-sulfur bacterium which carries a sensor vector having been transferred into a pigment mutant of a purple non-sulfur bacterium with the deletion of a gene encoding spheroidene monooxygenase, characterized in that, in the sensor vector as described above, an inductive promoter showing a specific response to the specific chemical is connected in an operable matter in the upstream of thegene encoding spheroidene monooxygenase.
Inventors:
YAGI KIYOHITO (JP)
KAWASE MASAYA (JP)
MAEDA ISAMU (JP)
YAMASHIRO HIDENORI (JP)
MIYASAKA HITOSHI (JP)
KAWASE MASAYA (JP)
MAEDA ISAMU (JP)
YAMASHIRO HIDENORI (JP)
MIYASAKA HITOSHI (JP)
Application Number:
PCT/JP2005/015224
Publication Date:
March 02, 2006
Filing Date:
August 22, 2005
Export Citation:
Assignee:
KANSAI ELECTRIC POWER CO (JP)
UNIV OSAKA (JP)
YAGI KIYOHITO (JP)
KAWASE MASAYA (JP)
MAEDA ISAMU (JP)
YAMASHIRO HIDENORI (JP)
MIYASAKA HITOSHI (JP)
UNIV OSAKA (JP)
YAGI KIYOHITO (JP)
KAWASE MASAYA (JP)
MAEDA ISAMU (JP)
YAMASHIRO HIDENORI (JP)
MIYASAKA HITOSHI (JP)
International Classes:
C12N15/09; C12Q1/02; C12Q1/26; C12Q1/68; (IPC1-7): C12Q1/68; C12N15/09; C12Q1/02; C12Q1/26
Other References:
YUASA K AND SUGIYAMA M.: "Bleomycin-induced beta-lactamase overexpression in Escherichia coli carrying a bleomycin-resistance gene from Streptomyces verticillus and its application to screen bleomycin analogues.", FEMS MICROBIOLOGY LETTERS., vol. 132, 1995, pages 61 - 66, XP002994392
ARMSTRONG G A ET AL: "Nucleotide sequence, organization, and nature of the protein products of the carotenoid biosynthesis gene cluster of Rhodobacter capsulatus.", MOL GEN GENET., vol. 216, 1989, pages 254 - 268, XP000577174
CHOUDHARY M AND KAPLAN S.: "DNA sequence analysis of the photosynthesis region of Rhodobacter sphaeroides 2.4.1T.", NUCLEIC ACID RES., vol. 28, no. 4, 2000, pages 862 - 867, XP002994393
PTITSYN LR ET AL: "A biosensor for environmental genotoxin screening based on an SOS lux assay in recombinant Escherichia coli cells.", APPL ENVIRON MICROBIOL., vol. 63, no. 11, November 1997 (1997-11-01), pages 4377 - 4384, XP002994394
MCDEVITT C A ET AL: "Molecular analysis of dimethyl sulphide dehydrogenase from Rhodovulum sulfidophilum: its place in the dimethyl sulphoxide reductase family of microbial molybdopterin-containing enzymes.", MOL MICROBIOL., vol. 44, no. 6, 2002, pages 1575 - 1587, XP002994395
TAURIAINEN S ET AL: "Measurement of firefly luciferase reporter gene activity from cells and lysates using Escherichia coli arsenite and mercury sensors.", ANAL BIOCHEM., vol. 272, no. 2, 1999, pages 191 - 198, XP001124323
XU C AND ROSEN B P.: "Dimerization is essential for DNA binding and repression by the ArsR metalloregulatory protein of Escherichia coli.", J BIOL CHEM., vol. 272, no. 25, 20 June 1997 (1997-06-20), pages 15734 - 15738, XP002994396
SCOTT DL ET AL: "Genetically engineered bacteria: electrochemical sensing systems for antimonite and arsenite.", ANAL CHEM., vol. 69, no. 1, 1997, pages 16 - 20, XP001124325
See also references of EP 1795613A4
ARMSTRONG G A ET AL: "Nucleotide sequence, organization, and nature of the protein products of the carotenoid biosynthesis gene cluster of Rhodobacter capsulatus.", MOL GEN GENET., vol. 216, 1989, pages 254 - 268, XP000577174
CHOUDHARY M AND KAPLAN S.: "DNA sequence analysis of the photosynthesis region of Rhodobacter sphaeroides 2.4.1T.", NUCLEIC ACID RES., vol. 28, no. 4, 2000, pages 862 - 867, XP002994393
PTITSYN LR ET AL: "A biosensor for environmental genotoxin screening based on an SOS lux assay in recombinant Escherichia coli cells.", APPL ENVIRON MICROBIOL., vol. 63, no. 11, November 1997 (1997-11-01), pages 4377 - 4384, XP002994394
MCDEVITT C A ET AL: "Molecular analysis of dimethyl sulphide dehydrogenase from Rhodovulum sulfidophilum: its place in the dimethyl sulphoxide reductase family of microbial molybdopterin-containing enzymes.", MOL MICROBIOL., vol. 44, no. 6, 2002, pages 1575 - 1587, XP002994395
TAURIAINEN S ET AL: "Measurement of firefly luciferase reporter gene activity from cells and lysates using Escherichia coli arsenite and mercury sensors.", ANAL BIOCHEM., vol. 272, no. 2, 1999, pages 191 - 198, XP001124323
XU C AND ROSEN B P.: "Dimerization is essential for DNA binding and repression by the ArsR metalloregulatory protein of Escherichia coli.", J BIOL CHEM., vol. 272, no. 25, 20 June 1997 (1997-06-20), pages 15734 - 15738, XP002994396
SCOTT DL ET AL: "Genetically engineered bacteria: electrochemical sensing systems for antimonite and arsenite.", ANAL CHEM., vol. 69, no. 1, 1997, pages 16 - 20, XP001124325
See also references of EP 1795613A4
Attorney, Agent or Firm:
Kazahaya, Nobuaki (6-20 Tosabori 1-chome, Nishi-k, Osaka-shi Osaka 01, JP)
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