Title:
BISPECIFIC ANTIBODY HAVING NEUTRALIZING ACTIVITY AGAINST CORONAVIRUS, AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2022/105772
Kind Code:
A1
Abstract:
Provided are a bispecific antibody having a neutralizing activity against a coronavirus, and a use thereof. A neutralizing antibody which is against a coronavirus S protein and blocks the binding of the coronavirus S proteins to an ACE 2 receptor is provided to prevent and treat coronaviruses. Specifically provided is an antibody or polypeptide complex specifically binding to a coronavirus S protein, comprising: (a) a first epitope binding moiety comprising a heavy chain variable region VH and a light chain variable region VL, wherein the VH and VL form an antigen binding domain specifically binding to a first epitope of an S protein; and (b) a second epitope binding moiety comprising a single-domain antibody or a VHH fragment thereof specifically binding to a second epitope of the S protein, wherein the first and second epitope binding portions are fused to each other and are not identical to each other. Also provided is a polynucleotide for encoding an antibody or a polypeptide complex and a host cell comprising same, and a method for preparing an antibody or a polypeptide complex. The antibody or polypeptide complex can be used for preventing, treating, diagnosing, and/or detecting coronaviruses.
Inventors:
LANG GUOJUN (CN)
LIU CHANJUAN (CN)
SHAO JUNBIN (CN)
TAN YONGCONG (CN)
KONG CHAO (CN)
YAN RUN (CN)
YAN XINTIAN (CN)
HU YUHAO (CN)
LIU CHANJUAN (CN)
SHAO JUNBIN (CN)
TAN YONGCONG (CN)
KONG CHAO (CN)
YAN RUN (CN)
YAN XINTIAN (CN)
HU YUHAO (CN)
Application Number:
PCT/CN2021/131080
Publication Date:
May 27, 2022
Filing Date:
November 17, 2021
Export Citation:
Assignee:
SANYOU BIOPHARMACEUTICALS CO LTD (CN)
SHANGHAI ZJ BIO TECH CO LTD (CN)
SHANGHAI ZJ BIO TECH CO LTD (CN)
International Classes:
C07K16/46; A61K39/42; A61P31/14; C07K16/10; C12N15/13; G01N33/569
Domestic Patent References:
| WO2013135655A1 | 2013-09-19 |
Foreign References:
| CN111690058A | 2020-09-22 | |||
| CN111333722A | 2020-06-26 | |||
| CN111592594A | 2020-08-28 | |||
| CN111592595A | 2020-08-28 |
Other References:
ZHANG, FENG ET AL.: "Development in Bispecific Antibody", CHINESE JOURNAL OF PHARMACEUTICAL ANALYSIS, vol. 39, no. 1, 31 January 2019 (2019-01-31), pages 78 - 85, XP009522265
HUO JIANDONG; LE BAS AUDREY; RUZA REINIS R.; DUYVESTEYN HELEN M. E.; MIKOLAJEK HALINA; MALINAUSKAS TOMAS; TAN TIONG KIT; RIJAL PRA: "Neutralizing nanobodies bind SARS-CoV-2 spike RBD and block interaction with ACE2", NATURE STRUCTURAL & MOLECULAR BIOLOGY, vol. 27, no. 9, 1 January 1900 (1900-01-01), New York , pages 846 - 854, XP037398758, ISSN: 1545-9993, DOI: 10.1038/s41594-020-0469-6
HUO JIANDONG; LE BAS AUDREY; RUZA REINIS R.; DUYVESTEYN HELEN M. E.; MIKOLAJEK HALINA; MALINAUSKAS TOMAS; TAN TIONG KIT; RIJAL PRA: "Neutralizing nanobodies bind SARS-CoV-2 spike RBD and block interaction with ACE2", NATURE STRUCTURAL & MOLECULAR BIOLOGY, vol. 27, no. 9, 1 January 1900 (1900-01-01), New York , pages 846 - 854, XP037398758, ISSN: 1545-9993, DOI: 10.1038/s41594-020-0469-6
Attorney, Agent or Firm:
NTD UNIVATION INTELLECTUAL PROPERTY AGENCY LTD. (CN)
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