Title:
CARBON DOT-HEMOGLOBIN COMPOSITE FOR CHOLESTEROL DETECTION
Document Type and Number:
WIPO Patent Application WO/2017/155172
Kind Code:
A1
Abstract:
The present invention relates to a carbon dot-hemoglobin composite for cholesterol detection. Specifically, according to the present invention, disclosed is a carbon dot-hemoglobin composite for cholesterol detection, the composite comprising: carbon dots; and hemoglobin chelate-bound to surfaces of the carbon dots, wherein the fluorescence of the carbon dots is quenched through the conjugation with the hemoglobin, and the fluorescence of the carbon dots is enhanced by the reaction with cholesterol, thereby detecting cholesterol.
Inventors:
PARK SOO YOUNG (KR)
BUI THI TRANG (KR)
BUI THI TRANG (KR)
Application Number:
PCT/KR2016/009440
Publication Date:
September 14, 2017
Filing Date:
August 25, 2016
Export Citation:
Assignee:
KYUNGPOOK NAT UNIV INDUSTRY-ACADEMIC COOP FOUND (KR)
International Classes:
G01N33/58; G01N21/64; G01N33/72; G01N33/92
Other References:
BUI, THI TRANG ET AL.: "A Fluorescent Biosensor for the Detection of Hemoglobin by Using Carbon Dots", THE POLYMER SOCIETY OF KOREA , ABSTRACTS PRESENTED AT 2015 AUTUMN MEETING, October 2015 (2015-10-01), pages 102
HE, XUEWEN ET AL.: "One-step Instant Synthesis of Protein-conjugated Quantum Dots at Room Temperature", SCIENTIFIC REPORTS, vol. 3, 2 October 2013 (2013-10-02), pages 2825, XP055414768
SZEBENI, JANOS ET AL.: "Interaction of Hemoglobin Derivatives with Liposomes. Membrane Cholesterol Protects against the Changes of Hemoglobin", BIOCHEMISTRY, vol. 27, 1988, pages 6425 - 6434, XP001030799
HU , DE-HONG ET AL.: "Study on the Interation between CsSe Quantum Dots and Hemo- globin", SPECTROCHIMICA ACTA, vol. 69, 2008, pages 830 - 834, XP022496156
SOUZA, TAMERA T. L. ET AL.: "Use of Hemoglobin as Alternative to Peroxidases in Cholesterol Amperometric Biosensors", SENSORS AND ACTUATORS B, vol. 178, 2013, pages 101 - 106, XP028993234
BERGERON, RAYMOND J. ET AL.: "Cholestarine and Ergostatetraene as in Vivo and in Vitro Membrane and Lipoprotein Probes", JOURNAL OF LIPID RESEARCH, vol. 23, 1982, pages 391 - 404, XP055414770
MILAN, NIKOLIC ET AL.: "Cholesterols Bound to Hemoglobin in Normal Human Erythro cytes: a New Form of Cholesterol in Circulation?", CLINICAL BIOCHEMISTRY, vol. 37, 2004, pages 22 - 26, XP055414774
HUANG, SHAN ET AL.: "A Carbon Dots Based Fluorescent Probe for Selective and Sensitive Detection of Hemoglobin", SENSORS AND ACTUATORS B: CHEMICAL, vol. 221, 2015, pages 1215 - 1222, XP029282964
BARATI, ALI ET AL.: "Hemoglobin Detection Using Carbon Dots as a Fluorescence Probe", BIO-SENSORS AND BIOELECTRONICS, vol. 71, 2015, pages 470 - 475, XP055414777
BUI, THI TRANG ET AL.: "A Carbon Dot-hemoglobin Complex-based Biosensor for Cholesterol Detection", GREEN CHEMISTRY, vol. 18, 3 May 2016 (2016-05-03), pages 4245 - 4253, XP055414779
KIM, SUNG WOOK ET AL.: "A Fluorescent Biosensor for the Detection of Hemoglobin by Using Carbon Dots", THE KOREAN SOCIETY OF INDUSTRIAL AND ENGINEERING CHEMISTRY 2016 SPRING CONFERENCE, vol. 20, no. 1, 2 May 2016 (2016-05-02)
HE, XUEWEN ET AL.: "One-step Instant Synthesis of Protein-conjugated Quantum Dots at Room Temperature", SCIENTIFIC REPORTS, vol. 3, 2 October 2013 (2013-10-02), pages 2825, XP055414768
SZEBENI, JANOS ET AL.: "Interaction of Hemoglobin Derivatives with Liposomes. Membrane Cholesterol Protects against the Changes of Hemoglobin", BIOCHEMISTRY, vol. 27, 1988, pages 6425 - 6434, XP001030799
HU , DE-HONG ET AL.: "Study on the Interation between CsSe Quantum Dots and Hemo- globin", SPECTROCHIMICA ACTA, vol. 69, 2008, pages 830 - 834, XP022496156
SOUZA, TAMERA T. L. ET AL.: "Use of Hemoglobin as Alternative to Peroxidases in Cholesterol Amperometric Biosensors", SENSORS AND ACTUATORS B, vol. 178, 2013, pages 101 - 106, XP028993234
BERGERON, RAYMOND J. ET AL.: "Cholestarine and Ergostatetraene as in Vivo and in Vitro Membrane and Lipoprotein Probes", JOURNAL OF LIPID RESEARCH, vol. 23, 1982, pages 391 - 404, XP055414770
MILAN, NIKOLIC ET AL.: "Cholesterols Bound to Hemoglobin in Normal Human Erythro cytes: a New Form of Cholesterol in Circulation?", CLINICAL BIOCHEMISTRY, vol. 37, 2004, pages 22 - 26, XP055414774
HUANG, SHAN ET AL.: "A Carbon Dots Based Fluorescent Probe for Selective and Sensitive Detection of Hemoglobin", SENSORS AND ACTUATORS B: CHEMICAL, vol. 221, 2015, pages 1215 - 1222, XP029282964
BARATI, ALI ET AL.: "Hemoglobin Detection Using Carbon Dots as a Fluorescence Probe", BIO-SENSORS AND BIOELECTRONICS, vol. 71, 2015, pages 470 - 475, XP055414777
BUI, THI TRANG ET AL.: "A Carbon Dot-hemoglobin Complex-based Biosensor for Cholesterol Detection", GREEN CHEMISTRY, vol. 18, 3 May 2016 (2016-05-03), pages 4245 - 4253, XP055414779
KIM, SUNG WOOK ET AL.: "A Fluorescent Biosensor for the Detection of Hemoglobin by Using Carbon Dots", THE KOREAN SOCIETY OF INDUSTRIAL AND ENGINEERING CHEMISTRY 2016 SPRING CONFERENCE, vol. 20, no. 1, 2 May 2016 (2016-05-02)
Attorney, Agent or Firm:
KIM, Sung-Hyun (KR)
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