Title:
CELL-ADHESIVE PHOTOCONTROLLABLE BASE MATERIAL
Document Type and Number:
WIPO Patent Application WO/2012/132551
Kind Code:
A1
Abstract:
The present invention makes it possible to more easily work in real-time to analyze, fractionate, and culture cells while the cells are alive, and possible to culture while also removing unwanted cells to purify the cell culture. The present invention also enhances the purity, recovery rate, and viability of desired cells fractionated and analyzed from the cell culture. Use is made of a cell-adhesive photocontrollable base material whereby a photolabile group including an O-nitrobenzyl backbone undergoes bond dissociation due to light irradiation and the surface at the irradiated portion is irreversibly changed from a cell-adhesive base material to a non-cell-adhesive base material. Cell images are detected and analyzed to obtain positional information on desired cells. On the basis of this information, a cut is made by a second light irradiation between cells and in the cell-adhesive photocontrollable base material. Meanwhile, the surface of the base material being changed from cell-adhesive to non-cell-adhesive, peeling occurs between the cells and the base material. This makes it possible to analyze and fractionate the cells while the cells are still alive.
Inventors:
KONNO TOMOHIRO (JP)
ISHIHARA KAZUHIKO (JP)
BYAMBAA BATZAYA (JP)
SUGIYAMA HISASHI (JP)
OZAWA SATOSHI (JP)
ISHIHARA KAZUHIKO (JP)
BYAMBAA BATZAYA (JP)
SUGIYAMA HISASHI (JP)
OZAWA SATOSHI (JP)
Application Number:
PCT/JP2012/052597
Publication Date:
October 04, 2012
Filing Date:
February 06, 2012
Export Citation:
Assignee:
UNIV TOKYO (JP)
HITACHI HIGH TECH CORP (JP)
KONNO TOMOHIRO (JP)
ISHIHARA KAZUHIKO (JP)
BYAMBAA BATZAYA (JP)
SUGIYAMA HISASHI (JP)
OZAWA SATOSHI (JP)
HITACHI HIGH TECH CORP (JP)
KONNO TOMOHIRO (JP)
ISHIHARA KAZUHIKO (JP)
BYAMBAA BATZAYA (JP)
SUGIYAMA HISASHI (JP)
OZAWA SATOSHI (JP)
International Classes:
C12M1/14
Domestic Patent References:
| WO2011058721A1 | 2011-05-19 |
Foreign References:
| JP2006006214A | 2006-01-12 | |||
| JP2009065945A | 2009-04-02 | |||
| JP2003339373A | 2003-12-02 | |||
| JP2008167695A | 2008-07-24 | |||
| JP2003321479A | 2003-11-11 | |||
| JP3975266B2 | 2007-09-12 | |||
| JP3472723B2 | 2003-12-02 | |||
| JP2004170930A | 2004-06-17 | |||
| JP2008167695A | 2008-07-24 | |||
| JP2011076462A | 2011-04-14 |
Other References:
WIRKNER, M. ET AL.: "Triggered cell release from materials using bioadhesive photocleavable linkers.", ADV. MATER., vol. 23, no. 34, 8 September 2011 (2011-09-08), pages 3907 - 3910, XP055126777
KAZUHIKO ISHIHARA; SEITAI ZAIRYO, BIOCOMPATIBLE MATERIAL, vol. 18, no. 1, 2000, pages 33
Y. ARIMA ET AL., J. METER. CHEM., vol. 17, 2007, pages 4079
Y. ARIMA ET AL., BIOMATERIALS, vol. 28, 2007, pages 3074
M. N. YOUSAF ET AL., PNAS, vol. 98, no. 11, 2001, pages 5992
TOSHIAKI FURUTA, KOGAKU (OPTICS, vol. 34, no. 4, 2005, pages 213
J. EDAHIRO ET AL., BIOMACROMOLECULES, vol. 6, no. 2, 2005, pages 970
J. NAKANISHI ET AL., ANALYTICAL SCIENCES, vol. 24, 2008, pages 67
See also references of EP 2692849A4
KAZUHIKO ISHIHARA; SEITAI ZAIRYO, BIOCOMPATIBLE MATERIAL, vol. 18, no. 1, 2000, pages 33
Y. ARIMA ET AL., J. METER. CHEM., vol. 17, 2007, pages 4079
Y. ARIMA ET AL., BIOMATERIALS, vol. 28, 2007, pages 3074
M. N. YOUSAF ET AL., PNAS, vol. 98, no. 11, 2001, pages 5992
TOSHIAKI FURUTA, KOGAKU (OPTICS, vol. 34, no. 4, 2005, pages 213
J. EDAHIRO ET AL., BIOMACROMOLECULES, vol. 6, no. 2, 2005, pages 970
J. NAKANISHI ET AL., ANALYTICAL SCIENCES, vol. 24, 2008, pages 67
See also references of EP 2692849A4
Attorney, Agent or Firm:
HIRAKI Yusuke et al. (JP)
Yusuke Hiraki (JP)
Yusuke Hiraki (JP)
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Claims: