Title:
CELL BASED ASSAY FOR MEASUREMENT OF BIOLOGICAL ACTIVITY OF ANTI TSHR AUTOIMMUNE ANTIBODIES
Document Type and Number:
WIPO Patent Application WO/2012/028654
Kind Code:
A3
Abstract:
The invention relates to a bioassay for the determination of biological activity of stimulating autoimmune antibodies directed against the TSHR receptor comprising the steps of a) incubating TSHR expressing cells with a patient sample, b) detecting the intensity of a signal in the supernatant of the cells that is caused by a change of cAMP concentration in the cells resulting from TSHR stimulation, c) determining the stimulating activity of the anti TSHR autoimmune antibodies by comparing the signal intensity of step b) with the signal intensity obtained after incubation of a first reference sample with TSHR expressing cells; the invention also refers to a bioassay for the determination of the biological activity of blocking autoimmune antibodies directed against the thyroid stimulating hormone receptor (TSHR), comprising the steps of a) incubating TSHR expressing cells with a patient sample, b) detecting the intensity of a signal in the supernatant of the cells that is caused by a change of cAMP concentration in the cells resulting from TSHR stimulation, c) determining the blocking/stimulation activity of the anti TSHR auto antibodies by comparing the signal intensity of step b) with the signal intensities obtained after incubation of a first and a second reference sample with TSHR expressing cells, wherein the incubation sample of step a) and the second reference sample contain the same amount of a TSHR stimulating agent.
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Inventors:
LOOS ULRICH (DE)
ZEIGER DIMITRIJ (DE)
ZEIGER DIMITRIJ (DE)
Application Number:
PCT/EP2011/065035
Publication Date:
July 02, 2015
Filing Date:
August 31, 2011
Export Citation:
Assignee:
KRELO GMBH MEDICAL DIAGNOSTICS (DE)
LOOS ULRICH (DE)
ZEIGER DIMITRIJ (DE)
LOOS ULRICH (DE)
ZEIGER DIMITRIJ (DE)
International Classes:
G01N33/50
Domestic Patent References:
| WO1999016902A1 | 1999-04-08 |
Other References:
WATSON P F ET AL: "A new chemiluminescent assay for the rapid detection of thyroid stimulating antibodies in Graves' disease", CLINICAL ENDOCRINOLOGY, BLACKWELL SCIENTIFIC PUBLICATIONS, OXFORD, GB, vol. 49, 1 November 1998 (1998-11-01), pages 577 - 581, XP002089745, ISSN: 0300-0664, DOI: 10.1046/J.1365-2265.1998.00619.X
S. D. LYTTON ET AL: "A Novel Thyroid Stimulating Immunoglobulin Bioassay Is a Functional Indicator of Activity and Severity of Graves' Orbitopathy", JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, vol. 95, no. 5, 1 May 2010 (2010-05-01), pages 2123 - 2131, XP055015052, ISSN: 0021-972X, DOI: 10.1210/jc.2009-2470
ANONYMOUS: "Pathway Profiling SystemUser Manual", 23 February 2009 (2009-02-23), XP002665967, Retrieved from the Internet [retrieved on 20111216]
LYTTON S D ET AL: "Bioassays for TSH-receptor autoantibodies: An update", AUTOIMMUNITY REVIEWS, ELSEVIER, AMSTERDAM, NL, vol. 10, no. 2, 1 December 2010 (2010-12-01), pages 116 - 122, XP027506586, ISSN: 1568-9972, [retrieved on 20101118], DOI: 10.1016/J.AUTREV.2010.08.018
S. D. LYTTON ET AL: "A Novel Thyroid Stimulating Immunoglobulin Bioassay Is a Functional Indicator of Activity and Severity of Graves' Orbitopathy", JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, vol. 95, no. 5, 1 May 2010 (2010-05-01), pages 2123 - 2131, XP055015052, ISSN: 0021-972X, DOI: 10.1210/jc.2009-2470
ANONYMOUS: "Pathway Profiling SystemUser Manual", 23 February 2009 (2009-02-23), XP002665967, Retrieved from the Internet
LYTTON S D ET AL: "Bioassays for TSH-receptor autoantibodies: An update", AUTOIMMUNITY REVIEWS, ELSEVIER, AMSTERDAM, NL, vol. 10, no. 2, 1 December 2010 (2010-12-01), pages 116 - 122, XP027506586, ISSN: 1568-9972, [retrieved on 20101118], DOI: 10.1016/J.AUTREV.2010.08.018
Attorney, Agent or Firm:
COHAUSZ & FLORACK (Düsseldorf, DE)
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