Title:
CELL CLUSTER COMPRISING PLURAL KINDS OF CELLS DERIVED FROM SOMA WITH ABILITY TO FORM PRIMITIVE ORGAN-LIKE STRUCTURE
Document Type and Number:
WIPO Patent Application WO/2008/001938
Kind Code:
A1
Abstract:
It is intended to provide a method for producing a cell cluster comprising plural kinds
of somatic cells derived from soma with an ability to form a primitive organ-like structure,
comprising preparing culture media containing the plural kinds of somatic cells,
mixing the culture media of the plural kinds of somatic cells and then adding a
Wnt signaling activator to the mixed cell culture medium, subjecting the culture
medium containing the Wnt signaling activator to nonplanar contact culture
for a predetermined period, replacing the medium of the culture subjected to
the nonplanar contact culture with a medium not containing the Wnt signaling activator
and further culturing it for a predetermined period, wherein at least one kind
of the plural kinds of somatic cells maintains an undifferentiated state.
Inventors:
FUJIWARA SHIGEYOSHI (JP)
KISHIMOTO JIRO (JP)
SOMA TSUTOMU (JP)
KISHIMOTO JIRO (JP)
SOMA TSUTOMU (JP)
Application Number:
PCT/JP2007/063333
Publication Date:
January 03, 2008
Filing Date:
June 27, 2007
Export Citation:
Assignee:
SHISEIDO CO LTD (JP)
FUJIWARA SHIGEYOSHI (JP)
KISHIMOTO JIRO (JP)
SOMA TSUTOMU (JP)
FUJIWARA SHIGEYOSHI (JP)
KISHIMOTO JIRO (JP)
SOMA TSUTOMU (JP)
International Classes:
A61L27/00; C12N5/071; C12N5/077
Other References:
SOMA T. ET AL.: "Mohatsu Kenkyu no Saikin no Chiken to Mohatsu Saisei", ORGAN. BIOL., vol. 12, no. 4, 2005, pages 311 - 322, XP003020308
LICHTI U. ET AL.: "In vivo regulation of murine hair growth: Insights from grafting defined cell populations onto nude mice", J. INVEST. DERMATOL., vol. 101, no. 1, SUPPL., 1993, pages 124S - 129S, XP009056470
KISHIMOTO J. ET AL.: "Selective activation of the versican promoter by epithelial-mesenchymal interactions during hair follicle development", PROC. NATL. ACAD. SCI. USA, vol. 96, 1999, pages 7336 - 7341, XP002925309
KISHIMOTO J.: "Wnt Signal ga Mocho Yudono o Iji suru", EXPERIMENTAL MEDICINE, vol. 18, no. 13, 2000, pages 1817 - 1820, XP003020309
KISHIMOTO J. ET AL.: "Wnt signaling maintains the hair-inducing activity of the dermal papilla", GENE DEV., vol. 14, 2000, pages 1181 - 1185, XP002942353
SATO N.: "Wnt Signal no Kasseika ni yoru Hito Oyobi Mouse ES Saibo no Mibunka Jotai no Iji", EXPERIMENTAL MEDICINE, vol. 22, no. 7, 2004, pages 962 - 965, XP003020310
BULL J.J. ET AL.: "Contrasting localization of c-Myc with other Myc superfamily transcription factors in the human hair follicle and during the hair growth cycle", J. INVEST. DERMATOL., vol. 116, no. 4, 2001, pages 617 - 622, XP003020311
WEGER N. ET AL.: "Igbfp3 modulates cell proliferation in the hair follicle", J. INVEST. DERMATOL., vol. 125, no. 4, 2005, pages 847 - 849, XP003020312
See also references of EP 2034011A4
CELL, vol. 6, no. 13, November 1975 (1975-11-01), pages 331 - 43
NOBORU SATO ET AL., NATURE MEDICINE, vol. 10, no. 1, January 2004 (2004-01-01)
KELLER, G.M. ET AL., CURR. OPIN. CELL BIOL., vol. 7, 1995, pages 862 - 869
LYNCH ET AL., J. CELL BIOL., vol. 103, 1986, pages 2593
SANO, S. ET AL., NATURE MED., vol. 11, 2005, pages 43 - 49
SATO, N. ET AL., NAT. MED. 2004, JAN, vol. 10, no. 1, 21 December 2003 (2003-12-21), pages 55 - 63
KISHIMOTO ET AL., PROC. NATL. ACAD. SCI. USA, 1999, pages 7336 - 7341
LUTZ ET AL., SKIN PHARMACOL., vol. 7, 1994, pages 101
PAUS ET AL., LAB. INVEST., vol. 60, 1989, pages 365
TAYLOR ET AL., J. INVEST. DERMATOL., vol. 100, 1993, pages 237
HENS ET AL., DEVELOPMENT, vol. 134, 2007, pages 1221
BULL, J.J. ET AL., INVEST. DERMATOL., vol. 116, 2001, pages 617
WEGER ET AL., J. INVEST. DERMATOL., vol. 125, 2005, pages 847
SCHLAKE ET AL., GENE EXPR. PATTERNS, vol. 4, 2004, pages 141
LICHTI U. ET AL.: "In vivo regulation of murine hair growth: Insights from grafting defined cell populations onto nude mice", J. INVEST. DERMATOL., vol. 101, no. 1, SUPPL., 1993, pages 124S - 129S, XP009056470
KISHIMOTO J. ET AL.: "Selective activation of the versican promoter by epithelial-mesenchymal interactions during hair follicle development", PROC. NATL. ACAD. SCI. USA, vol. 96, 1999, pages 7336 - 7341, XP002925309
KISHIMOTO J.: "Wnt Signal ga Mocho Yudono o Iji suru", EXPERIMENTAL MEDICINE, vol. 18, no. 13, 2000, pages 1817 - 1820, XP003020309
KISHIMOTO J. ET AL.: "Wnt signaling maintains the hair-inducing activity of the dermal papilla", GENE DEV., vol. 14, 2000, pages 1181 - 1185, XP002942353
SATO N.: "Wnt Signal no Kasseika ni yoru Hito Oyobi Mouse ES Saibo no Mibunka Jotai no Iji", EXPERIMENTAL MEDICINE, vol. 22, no. 7, 2004, pages 962 - 965, XP003020310
BULL J.J. ET AL.: "Contrasting localization of c-Myc with other Myc superfamily transcription factors in the human hair follicle and during the hair growth cycle", J. INVEST. DERMATOL., vol. 116, no. 4, 2001, pages 617 - 622, XP003020311
WEGER N. ET AL.: "Igbfp3 modulates cell proliferation in the hair follicle", J. INVEST. DERMATOL., vol. 125, no. 4, 2005, pages 847 - 849, XP003020312
See also references of EP 2034011A4
CELL, vol. 6, no. 13, November 1975 (1975-11-01), pages 331 - 43
NOBORU SATO ET AL., NATURE MEDICINE, vol. 10, no. 1, January 2004 (2004-01-01)
KELLER, G.M. ET AL., CURR. OPIN. CELL BIOL., vol. 7, 1995, pages 862 - 869
LYNCH ET AL., J. CELL BIOL., vol. 103, 1986, pages 2593
SANO, S. ET AL., NATURE MED., vol. 11, 2005, pages 43 - 49
SATO, N. ET AL., NAT. MED. 2004, JAN, vol. 10, no. 1, 21 December 2003 (2003-12-21), pages 55 - 63
KISHIMOTO ET AL., PROC. NATL. ACAD. SCI. USA, 1999, pages 7336 - 7341
LUTZ ET AL., SKIN PHARMACOL., vol. 7, 1994, pages 101
PAUS ET AL., LAB. INVEST., vol. 60, 1989, pages 365
TAYLOR ET AL., J. INVEST. DERMATOL., vol. 100, 1993, pages 237
HENS ET AL., DEVELOPMENT, vol. 134, 2007, pages 1221
BULL, J.J. ET AL., INVEST. DERMATOL., vol. 116, 2001, pages 617
WEGER ET AL., J. INVEST. DERMATOL., vol. 125, 2005, pages 847
SCHLAKE ET AL., GENE EXPR. PATTERNS, vol. 4, 2004, pages 141
Attorney, Agent or Firm:
AOKI, Atsushi et al. (Toranomon 37 Mori Bldg. 5-1,Toranomon 3-chome, Minato-ku, Tokyo 23, JP)
Download PDF: