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Title:
CELL CULTURE METHOD
Document Type and Number:
WIPO Patent Application WO/2020/071209
Kind Code:
A1
Abstract:
A method wherein cells are cultured while stirring a cell suspension, which contains stem cells and a culture medium, by rotating at least one stirring blade. In this method: the viscosity of the culture medium is controlled to 10-1000 mPa•s inclusive; the product of the stirring blade length and the number of the stirring blade(s) is controlled to 20-500 mm inclusive; and the average rotational speed of the stirring blade(s) is controlled to 0.01-5/min inclusive.

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Inventors:
GOTO SHUN (JP)
TAKAYAMA HIDETOSHI (JP)
Application Number:
PCT/JP2019/037590
Publication Date:
April 09, 2020
Filing Date:
September 25, 2019
Export Citation:
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Assignee:
FUJIFILM CORP (JP)
International Classes:
C12N5/074; C12N5/0735
Domestic Patent References:
WO2017126647A12017-07-27
WO2014017513A12014-01-30
WO2017191775A12017-11-09
Foreign References:
JPH06105680A1994-04-19
Other References:
OTSUJI, TOMOMI ET AL: "Mass culture technique of human ES/iPS cells using functional macromolecule Fusion with physical chemistry and the regenerative therapy", CHEMISTRY, vol. 69, no. 9, 2014, pages 12 - 15
Attorney, Agent or Firm:
TAIYO, NAKAJIMA & KATO (JP)
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