CELL CULTURING METHOD, AND METHOD FOR PRODUCING USEFUL SUBSTANCE

WIPO Patent Application WO/2022/196710

A1

The present invention addresses the problem of providing: a cell culturing method whereby it becomes possible to prevent oxygen deficiency in cells when the cells are cultured at a high density; and a method for producing a useful substance utilizing the cell culturing method. According to the present invention, a cell culturing method is provided, in which the relationship among an agitation power per unit volume P/V, a dissolved oxygen concentration CL0 and a viable cell density VCD satisfies the requirement represented by formula 1 in cell culturing at a viable cell density of 60×106 cell/mL or more. Formula 1: 0.0059×exp[0.0243×(P/V)]×CL0/VCD ≧ 1.10×10-17 In the formula, CL0 represents a dissolved oxygen concentration [mol/mL]; VCD represents a viable cell density [cell/mL]: and P/V represents an agitation power per unit volume [W/m3] which is defined by a specified formula.

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INADA ATSUSHI (JP)
TANIGUCHI KOSUKE (JP)
TAKAHASHI NAOTO (JP)

PCT/JP2022/011766

September 22, 2022

March 16, 2022

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FUJIFILM CORP (JP)

C12N5/071; C12N1/00; C12N5/10; C12P21/00

JPH0838166A	1996-02-13
CN201678670U	2010-12-22
JP2015216886A	2015-12-07
JP2011092117A	2011-05-12
JP2016517691A	2016-06-20

SIKS & CO. (JP)

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