Title:
CELL DETERMINATION METHOD, CELL DETERMINATION DEVICE AND CELL DETERMINATION PROGRAM
Document Type and Number:
WIPO Patent Application WO/2017/047190
Kind Code:
A1
Abstract:
The present invention relates to a cell determination method that determines cell types on the basis of the glycogen content in the cells, the cell determination method comprising: an acquisition step of measuring the optical path length of the cell and acquiring optical path length data; a calculation step of calculating an optical path length index that correlates with the optical path length of the cell, based on the acquired optical path length data; a comparison step of comparing the calculated optical path length index and a threshold value; and a determination step of determining that the cell is of a cell type having a high intracellular glycogen content, or of a cell type having a low intracellular glycogen content, on the basis of the results of the comparison.
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Inventors:
FUKAMI TADASHI (JP)
YAMADA HIDENAO (JP)
YAMADA HIDENAO (JP)
Application Number:
PCT/JP2016/068549
Publication Date:
March 23, 2017
Filing Date:
June 22, 2016
Export Citation:
Assignee:
HAMAMATSU PHOTONICS KK (JP)
International Classes:
C12Q1/04; C12M1/34; G01N21/45; G02B21/00
Domestic Patent References:
| WO2015076042A1 | 2015-05-28 | |||
| WO2012147403A1 | 2012-11-01 |
Foreign References:
| JP2014039535A | 2014-03-06 | |||
| JP2013249298A | 2013-12-12 |
Other References:
ROTHENBERG MICHAEL E. ET AL.: "The Myc connection: ES cells and cancer", CELL, vol. 143, no. 2, 2010, pages 184 - 186, XP028931127
KIM JONGHWAN ET AL.: "Embryonic stem cell - specific signatures in cancer: insights into genomic regulatory networks and implications for medicine", GENOME MED., vol. 11, no. 75, March 2011 (2011-03-01), pages 1 - 8, XP055370449
JOHKURA KOHEI ET AL.: "Cytochemical and ultrastructural characterization of growing colonies of human embryonic stem cells", JOURNAL OF ANATOMY, vol. 205, no. 4, October 2004 (2004-10-01), pages 247 - 255, XP055370464
KONOROV STANISLAV O. ET AL.: "Absolute quantification of intracellular glycogen content in human embryonic stem cells with Raman microspectroscopy", ANAL.CHEM., vol. 83, no. 16, 15 August 2011 (2011-08-15), pages 6254 - 6258, XP055370466
KONOROV STANISLAV O. ET AL.: "Raman microscopy- based cytochemical investigations of potential niche-forming inhomogeneities present in human embryonic stem cell colonies", APPL.SPECTROSC., vol. 65, no. 9, 1 September 2011 (2011-09-01), pages 1009 - 1016, XP055370471
TOYOHIKO YAMAUCHI ET AL.: "Teiryo Iso Kenbikyo ni yoru Hito iPS Saibo no Hisenshoku Kansatsu", REGENERATIVE MEDICINE, vol. 15, 1 February 2016 (2016-02-01), pages 223
KIM JONGHWAN ET AL.: "Embryonic stem cell - specific signatures in cancer: insights into genomic regulatory networks and implications for medicine", GENOME MED., vol. 11, no. 75, March 2011 (2011-03-01), pages 1 - 8, XP055370449
JOHKURA KOHEI ET AL.: "Cytochemical and ultrastructural characterization of growing colonies of human embryonic stem cells", JOURNAL OF ANATOMY, vol. 205, no. 4, October 2004 (2004-10-01), pages 247 - 255, XP055370464
KONOROV STANISLAV O. ET AL.: "Absolute quantification of intracellular glycogen content in human embryonic stem cells with Raman microspectroscopy", ANAL.CHEM., vol. 83, no. 16, 15 August 2011 (2011-08-15), pages 6254 - 6258, XP055370466
KONOROV STANISLAV O. ET AL.: "Raman microscopy- based cytochemical investigations of potential niche-forming inhomogeneities present in human embryonic stem cell colonies", APPL.SPECTROSC., vol. 65, no. 9, 1 September 2011 (2011-09-01), pages 1009 - 1016, XP055370471
TOYOHIKO YAMAUCHI ET AL.: "Teiryo Iso Kenbikyo ni yoru Hito iPS Saibo no Hisenshoku Kansatsu", REGENERATIVE MEDICINE, vol. 15, 1 February 2016 (2016-02-01), pages 223
Attorney, Agent or Firm:
HASEGAWA Yoshiki et al. (JP)
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