Title:
CELL ENUMERATION
Document Type and Number:
WIPO Patent Application WO2003006956
Kind Code:
A3
Abstract:
The invention provides a method of enumerating the number of cells of a cell type in a cell sample by (a) counting the white blood cells in the cell sample to obtain the white blood cell population of the sample; (b) determining the proportion or percentage of the cells of the cell type in the white blood cell population in the sample; and (c) calculating the number of cells of the cell type in the sample. The cell type may be a lymphocyte sub-set selected from the group comprising CD4+ lymphocytes, CD 45 cells, CD19 cells, CD16 and CD56 positive cells, CD8 cells, CD3 cells or any combination thereof. The method is particularly useful in monitoring state or disease or condition where CD4+ lymphocytes or CD4+ T cells are monitored or counted.
Inventors:
GLENCROSS DEBORAH KIM (ZA)
Application Number:
PCT/IB2002/002725
Publication Date:
September 25, 2003
Filing Date:
July 11, 2002
Export Citation:
Assignee:
NAT HEALTH LAB SERVICE (ZA)
GLENCROSS DEBORAH KIM (ZA)
GLENCROSS DEBORAH KIM (ZA)
International Classes:
C12Q1/04; G01N33/49; G01N15/14; G01N33/50; G01N33/569; G01N15/00; G01N15/10; G01N; (IPC1-7): G01N33/50; G01N33/569
Foreign References:
| US5812419A | 1998-09-22 | |||
| US5776709A | 1998-07-07 | |||
| EP0470810B1 | 1996-04-10 | |||
| US5945293A | 1999-08-31 | |||
| US5064616A | 1991-11-12 | |||
| US3896307A | 1975-07-22 |
Other References:
CENTERS FOR DISEASE CONTROL (USA): "1997 revised guidelines for performing CD4+ T-cell determinations in persons infected with human immunodeficiency virus (HIV).", MORBIDITY AND MORTALITY WEEKLY REPORT, vol. 46, no. RR-2, 1997, pages I - II, 1-29, XP002237836, ISSN: 0149-2195
BRANDO B ET AL: "Cytofluorometric Methods for Assessing Absolute Numbers of Cell Subsets in Blood", CYTOMETRY, ALAN R. LISS, INC, XX, vol. 42, 2000, pages 327 - 346, XP002197095, ISSN: 0196-4763
NICHOLSON JANET K A ET AL: "Use of CD45 fluorescence and side-scatter characteristics for gating lymphocytes when using the whole blood lysis procedure and flow cytometry.", CYTOMETRY, vol. 26, no. 1, 1996, pages 16 - 21, XP009009076, ISSN: 0196-4763
SCHNIZLEIN-BICK CAROL T ET AL: "Evaluation of TruCount absolute-count tubes for determining CD4 and CD8 cell numbers in human immunodeficiency virus-positive adults.", CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, vol. 7, no. 3, May 2000 (2000-05-01), pages 336 - 343, XP002237837, ISSN: 1071-412X
LOKEN M R ET AL: "ESTABLISHING OPTIMAL LYMPHOCYTE GATES FOR IMMUNOPHENOTYPING BY FLOW CYTOMETRY", CYTOMETRY, ALAN LISS, NEW YORK, US, vol. 11, 1990, pages 453 - 459, XP000770385, ISSN: 0196-4763
BARNETT D ET AL: "Absolute CD4+ T-lymphocyte and CD34+ stem cell counts by single-platform flow cytometry: The way forward.", BRITISH JOURNAL OF HAEMATOLOGY, vol. 106, no. 4, 1999, pages 1059 - 1062, XP002237838, ISSN: 0007-1048
SHERMAN G G ET AL: "CD4T cell enumeration in HIV infection with limited resources", JOURNAL OF IMMUNOLOGICAL METHODS, ELSEVIER SCIENCE PUBLISHERS B.V.,AMSTERDAM, NL, vol. 222, no. 1-2, 1999, pages 209 - 217, XP004152442, ISSN: 0022-1759
BRANDO B ET AL: "Cytofluorometric Methods for Assessing Absolute Numbers of Cell Subsets in Blood", CYTOMETRY, ALAN R. LISS, INC, XX, vol. 42, 2000, pages 327 - 346, XP002197095, ISSN: 0196-4763
NICHOLSON JANET K A ET AL: "Use of CD45 fluorescence and side-scatter characteristics for gating lymphocytes when using the whole blood lysis procedure and flow cytometry.", CYTOMETRY, vol. 26, no. 1, 1996, pages 16 - 21, XP009009076, ISSN: 0196-4763
SCHNIZLEIN-BICK CAROL T ET AL: "Evaluation of TruCount absolute-count tubes for determining CD4 and CD8 cell numbers in human immunodeficiency virus-positive adults.", CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, vol. 7, no. 3, May 2000 (2000-05-01), pages 336 - 343, XP002237837, ISSN: 1071-412X
LOKEN M R ET AL: "ESTABLISHING OPTIMAL LYMPHOCYTE GATES FOR IMMUNOPHENOTYPING BY FLOW CYTOMETRY", CYTOMETRY, ALAN LISS, NEW YORK, US, vol. 11, 1990, pages 453 - 459, XP000770385, ISSN: 0196-4763
BARNETT D ET AL: "Absolute CD4+ T-lymphocyte and CD34+ stem cell counts by single-platform flow cytometry: The way forward.", BRITISH JOURNAL OF HAEMATOLOGY, vol. 106, no. 4, 1999, pages 1059 - 1062, XP002237838, ISSN: 0007-1048
SHERMAN G G ET AL: "CD4T cell enumeration in HIV infection with limited resources", JOURNAL OF IMMUNOLOGICAL METHODS, ELSEVIER SCIENCE PUBLISHERS B.V.,AMSTERDAM, NL, vol. 222, no. 1-2, 1999, pages 209 - 217, XP004152442, ISSN: 0022-1759
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