Title:
CELL EXPANSION LETHAL TOXIN AND DETECTION OF BACTERIUM BELONGING TO CAMPYLOBACTER GENUS THAT TARGETS THE SAME
Document Type and Number:
WIPO Patent Application WO/2005/054472
Kind Code:
A1
Abstract:
With respect to unknown CDT genes of C. coli and C. fetus, cloning and sequence determination have been successfully accomplished. Further, C. jejuni and C. fetus CDT comparison has been carried out, and primers specific to and common to both have been developed. It has been probed that the developed primers are applicable to the multiplex PCR method capable of simultaneously conducting easy and speedy determination of the presence of CDT of Campylobacter genus bacteria and identification of bacterial strain thereof and can further be utilized in the typing according to the PCR-RFLP method.
Inventors:
YAMASAKI SHINJI (JP)
ASAKURA MASAHIRO (JP)
ASAKURA MASAHIRO (JP)
Application Number:
PCT/JP2004/018042
Publication Date:
June 16, 2005
Filing Date:
December 03, 2004
Export Citation:
Assignee:
FUSO PHARMACEUTICAL IND (JP)
YAMASAKI SHINJI (JP)
ASAKURA MASAHIRO (JP)
YAMASAKI SHINJI (JP)
ASAKURA MASAHIRO (JP)
International Classes:
C07K14/205; C12Q1/68; (IPC1-7): C12N15/31; C12N1/15; C12N1/19; C12N1/21; C12N5/00; C07K14/205; C07K16/12; C12Q1/68; C12P21/02; G01N33/50; G01N33/569
Foreign References:
| JPH10508499A | 1998-08-25 |
Other References:
BANG D.D. ET AL: "PCR detection of seven virulence and toxin genes of Campylobacter jejuni and Campylobacter coli isolates from Danish pigs and cattle and cytolethal distending toxin productionof the isolates", J. APPL. MICROBIOL., vol. 94, no. 6, June 2003 (2003-06-01), pages 1003 - 1014, XP002988024
BANG D.D. ET AL: "Prevalence of cytolethal distending toxin (cdt) genes and CDT production in Campylobacter spp. isolated from Danish broilers", J. MED. MICROBIOL., vol. 50, no. 12, 2001, pages 1087 - 1094, XP002988025
EYIGOR A. ET AL: "Cytolethal distending toxin genes in Campylobacter jejuni and Campylobacter coli isolates: detection and analysis by PCR", J. CLIN. MICROBIOL., vol. 37, no. 5, May 1997 (1997-05-01), pages 1646 - 1650, XP002988026
EYIGOR A. ET AL: "Detection of cytolethal distending toxin antivity and cdt genes in Campylobacter spp. isolated from chicken carcasses", APPL. ENVIRON. MICROBIOL., vol. 65, no. 4, 1999, pages 1501 - 1505, XP008049259
VOLOKHOV D. ET AL: "Microarray-based identification of thermophilic Campylobacter jejuni, C. coli, C. lari, and C. upsaliensis", J. CLIN. MICROBIOL., vol. 41, no. 9, September 2003 (2003-09-01), pages 4071 - 4080, XP002988027
BANG D.D. ET AL: "Prevalence of cytolethal distending toxin (cdt) genes and CDT production in Campylobacter spp. isolated from Danish broilers", J. MED. MICROBIOL., vol. 50, no. 12, 2001, pages 1087 - 1094, XP002988025
EYIGOR A. ET AL: "Cytolethal distending toxin genes in Campylobacter jejuni and Campylobacter coli isolates: detection and analysis by PCR", J. CLIN. MICROBIOL., vol. 37, no. 5, May 1997 (1997-05-01), pages 1646 - 1650, XP002988026
EYIGOR A. ET AL: "Detection of cytolethal distending toxin antivity and cdt genes in Campylobacter spp. isolated from chicken carcasses", APPL. ENVIRON. MICROBIOL., vol. 65, no. 4, 1999, pages 1501 - 1505, XP008049259
VOLOKHOV D. ET AL: "Microarray-based identification of thermophilic Campylobacter jejuni, C. coli, C. lari, and C. upsaliensis", J. CLIN. MICROBIOL., vol. 41, no. 9, September 2003 (2003-09-01), pages 4071 - 4080, XP002988027
Attorney, Agent or Firm:
Shimizu, Hatsushi (1-1-1 Oroshi-machi, Tsuchiura-sh, Ibaraki 47, JP)
Download PDF: