Title:
CELL PREPARATION CONTAINING MESENCHYMAL STEM CELLS, AND METHOD FOR PRODUCING SAME
Document Type and Number:
WIPO Patent Application WO/2011/111787
Kind Code:
A1
Abstract:
Disclosed is a cell preparation, which contains mesenchymal stem cells that maintain immunosuppressive ability, and which is produced by serum-free or low-serum culture. Specifically disclosed is a method for producing a cell preparation containing mesenchymal stem cells, which is characterized by comprising: a proliferation step wherein mesenchymal stem cells are proliferated in a culture medium that contains FGF, PDGF, TGF-β, HGF, EGF, at least one phospholipid and at least one fatty acid; and a screening step wherein the mesenchymal stem cells after the proliferation step are screened for mesenchymal stem cells in which the immunosuppressive ability is maintained or improved.
Inventors:
TSUCHIYA TOSHIE
TSUJI KOICHIRO
KATO YUKIO (JP)
SHAO JIN CHANG (JP)
HARA MAIKO (JP)
TSUJI KOICHIRO
KATO YUKIO (JP)
SHAO JIN CHANG (JP)
HARA MAIKO (JP)
Application Number:
PCT/JP2011/055683
Publication Date:
September 15, 2011
Filing Date:
March 10, 2011
Export Citation:
Assignee:
TWO CELLS CO LTD (JP)
TSUCHIYA TOSHIE
TSUJI KOICHIRO
KATO YUKIO (JP)
SHAO JIN CHANG (JP)
HARA MAIKO (JP)
TSUCHIYA TOSHIE
TSUJI KOICHIRO
KATO YUKIO (JP)
SHAO JIN CHANG (JP)
HARA MAIKO (JP)
International Classes:
A61K35/12; A61K35/28; A61P37/06; C12N5/0775
Domestic Patent References:
| WO2007080919A1 | 2007-07-19 | |||
| WO2007080919A1 | 2007-07-19 |
Foreign References:
| JP2007536935A | 2007-12-20 |
Other References:
YUKIO KATO: "Mukessei Baichi de Kan'yokei Kansaibo no STEMNESS Bunshi Kiko o Kasseika suru: Kessei Saisei Iryo kara Mukessei Saisei Iryo eno Tenkan", JAPAN SOCIETY OF PLASTIC AND RECONSTRUCTIVE SURGERY KISO GAKUJUTSU SHUKAI PROGRAM SHOROKUSHU, vol. 18TH, 2009, pages 54 - 55
RUMI SAWADA ET AL.: "Hito Kotsuzui Yurai Kan'yokei Kansaibo no in vitro Baiyo-ji ni Okeru Idenshi Hatsugen no Henka ni Tsuite, Mukessei Baichi no Eikyo", REGENERATIVE MEDICINE, vol. 8, 2009, pages 248
CHRISTELLE DOUCET ET AL.: "Platelet Lysates Promote Mesenchymal Stem Cell Expansion: A Safety Substitute for Animal Serum in Cell- Based Therapy Applications", JOURNAL OF CELLULAR PHYSIOLOGY, vol. 205, 2005, pages 228 - 236, XP002454867
PANAGIOTA A. SOTIROPOULOU ET AL.: "Characterization of the Optimal Culture Conditions for Clinical Scale Production of Human Mesenchymal Stem Cells", STEM CELLS, vol. 24, 2006, pages 462 - 471, XP009066391
MASSIMO DI NICOLA ET AL.: "Human bone marrow stromal cells suppress T-lymphocyte proliferation induced by cellular or nonspecific mitogenic stimuli", BLOOD, vol. 99, no. 10, 2002, pages 3838 - 3843, XP002982803
See also references of EP 2545928A4
KATO YUKIO: "Proceedings of Fifth Iryokiki Forum", 2007, MEDICAL EQUIPMENT FORUM, pages: 33 - 35
KEATING, A., CELL STEM CELL, vol. 2, 2008, pages 106 - 108
CORCIONE, A., BLOOD, vol. 107, 2006, pages 367 - 372
RAMASAMY, R., TRANSPLANTATION, vol. 83, 2007, pages 71 - 76
AGGARWAL, S., BLOOD, vol. 105, 2005, pages 1815 - 1822
LE BLANC K., J INTERN MED., vol. 262, 2007, pages 509 - 525
DJOUAD, F., BLOOD, vol. 102, 2003, pages 3837 - 3844
RUMI SAWADA ET AL.: "Hito Kotsuzui Yurai Kan'yokei Kansaibo no in vitro Baiyo-ji ni Okeru Idenshi Hatsugen no Henka ni Tsuite, Mukessei Baichi no Eikyo", REGENERATIVE MEDICINE, vol. 8, 2009, pages 248
CHRISTELLE DOUCET ET AL.: "Platelet Lysates Promote Mesenchymal Stem Cell Expansion: A Safety Substitute for Animal Serum in Cell- Based Therapy Applications", JOURNAL OF CELLULAR PHYSIOLOGY, vol. 205, 2005, pages 228 - 236, XP002454867
PANAGIOTA A. SOTIROPOULOU ET AL.: "Characterization of the Optimal Culture Conditions for Clinical Scale Production of Human Mesenchymal Stem Cells", STEM CELLS, vol. 24, 2006, pages 462 - 471, XP009066391
MASSIMO DI NICOLA ET AL.: "Human bone marrow stromal cells suppress T-lymphocyte proliferation induced by cellular or nonspecific mitogenic stimuli", BLOOD, vol. 99, no. 10, 2002, pages 3838 - 3843, XP002982803
See also references of EP 2545928A4
KATO YUKIO: "Proceedings of Fifth Iryokiki Forum", 2007, MEDICAL EQUIPMENT FORUM, pages: 33 - 35
KEATING, A., CELL STEM CELL, vol. 2, 2008, pages 106 - 108
CORCIONE, A., BLOOD, vol. 107, 2006, pages 367 - 372
RAMASAMY, R., TRANSPLANTATION, vol. 83, 2007, pages 71 - 76
AGGARWAL, S., BLOOD, vol. 105, 2005, pages 1815 - 1822
LE BLANC K., J INTERN MED., vol. 262, 2007, pages 509 - 525
DJOUAD, F., BLOOD, vol. 102, 2003, pages 3837 - 3844
Attorney, Agent or Firm:
HARAKENZO WORLD PATENT & TRADEMARK (JP)
Patent business corporation Hara [Kenzo] international patent firm (JP)
Patent business corporation Hara [Kenzo] international patent firm (JP)
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