Title:
CHIMERIC PROTEIN AND ITS USE IN ELECTRON TRANSFER METHODS
Document Type and Number:
WIPO Patent Application WO2003014721
Kind Code:
A3
Abstract:
A chimeric protein comprises a redox catalytic domain from one source and an electron transfer domain from a different source. The protein is used in a method in which a substrate for the redox catalytic domain is acted on, electrons are transferred between the redox catalytic domain and the electron transfer domain and between the electron transfer domain and an electrode. The flow of current or potential at the electrode may be monitored to determine the presence or amount of a substrate which is an analyte of interest. Alternatively current max be driven through the electrode to drive reaction of the substrate, for instance to detoxify samples. The redox catalytic domain is suitably derived from a cytochrome P450, and the electron transfer domain may be flavodoxin.
Inventors:
GILARDI GIANFRANCO (GB)
CASS ANTHONY E G (GB)
CASS ANTHONY E G (GB)
Application Number:
PCT/GB2002/003596
Publication Date:
May 30, 2003
Filing Date:
August 05, 2002
Export Citation:
Assignee:
NANOBIODESIGN LTD (GB)
GILARDI GIANFRANCO (GB)
CASS ANTHONY E G (GB)
GILARDI GIANFRANCO (GB)
CASS ANTHONY E G (GB)
International Classes:
C07K19/00; C12N9/02; G01N27/416; C12Q1/00; C12Q1/26; G01N27/327; (IPC1-7): G01N27/327; C12Q1/00
Domestic Patent References:
| WO1999027356A1 | 1999-06-03 |
Other References:
SADEGHI S J ET AL: "Engineering artificial redox chains by molecular Lego", FARADAY DISCUSSIONS, vol. 116, 2000, pages 135 - 153, XP002193724
HEERING H A ET AL: "Direct detection and measurement of electron relays in a multicentered enzyme: voltammetry of electrode-surface films of E. coli fumarate, an iron-sulfur flavoprotein", JOURNAL AMERICAN CHEMICAL SOCIETY, vol. 119, 1997, pages 11628 - 11638, XP002193725
GILARDI G ET AL: "Molecular Lego: design of molecular assemblies of P450 enzymes for nanobiotechnology", BIOSENSORS & BIOELECTRONICS, vol. 17, 2002, pages 133 - 145, XP001066229
HEERING H A ET AL: "Direct detection and measurement of electron relays in a multicentered enzyme: voltammetry of electrode-surface films of E. coli fumarate, an iron-sulfur flavoprotein", JOURNAL AMERICAN CHEMICAL SOCIETY, vol. 119, 1997, pages 11628 - 11638, XP002193725
GILARDI G ET AL: "Molecular Lego: design of molecular assemblies of P450 enzymes for nanobiotechnology", BIOSENSORS & BIOELECTRONICS, vol. 17, 2002, pages 133 - 145, XP001066229
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