Title:
CIRCULAR RNA, VACCINE CONTAINING CIRCULAR RNA, AND KIT FOR DETECTING NOVEL CORONAVIRUS NEUTRALIZING ANTIBODY
Document Type and Number:
WIPO Patent Application WO/2022/116528
Kind Code:
A1
Abstract:
The present disclosure belongs to the technical fields of molecular biology and bioengineering, and relates to a circular RNA, a vaccine containing the circular RNA, and a kit for detecting a novel coronavirus neutralizing antibody. A recombinant nucleic acid molecule of the present disclosure is transcribed to form a circular RNA containing a specific IRES element, so that the protein expression level of the circular RNA in a eukaryotic cell can be improved, thereby realizing the efficient and persistent expression of the protein. The circular RNA of the present disclosure can express an RBD antigen in a host cell and enhance the antiviral immune response generated by the host, and the circular RNA has a high effectiveness and protective effect as a vaccine. The recombinant receptor binding protein and the recombinant receptor protein of the present disclosure can simulate the natural protein conformation of the RBD protein and ACE2 protein, thereby improving the sensitivity, specificity and stability of detection of the novel coronavirus neutralizing antibody.
Inventors:
SUN ZHENHUA (CN)
ZUO CHIJIAN (CN)
ZHU JIAFENG (CN)
GAO SHUFENG (CN)
LI NA (CN)
ZUO CHIJIAN (CN)
ZHU JIAFENG (CN)
GAO SHUFENG (CN)
LI NA (CN)
Application Number:
PCT/CN2021/103688
Publication Date:
June 09, 2022
Filing Date:
June 30, 2021
Export Citation:
Assignee:
SUZHOU CUREMED BIOMEDICAL TECH CO LTD (CN)
International Classes:
A61K39/215; C12N15/79; C07K19/00; C12N5/10; C12N15/85; G01N33/569
Domestic Patent References:
| WO2019236673A1 | 2019-12-12 |
Foreign References:
| CN111944064A | 2020-11-17 | |||
| CN112707968A | 2021-04-27 | |||
| CN112481289A | 2021-03-12 |
Other References:
DATABASE NUCLEOTIDE 29 December 2003 (2003-12-29), ANONYMOUS: "Human echovirus 24 strain DeCamp complete genome", XP055937794, retrieved from GENBANK Database accession no. AY302548
DATABASE NUCLEOTIDE 29 December 2003 (2003-12-29), ANONYMOUS: "Human echovirus 29 strain JV-10 complete genome", XP055937797, retrieved from GENBANK Database accession no. AY302552
DATABASE NUCLEOTIDE 29 December 2003 (2003-12-29), ANONYMOUS: "Human echovirus 33 strain Toluca-3 complete genome", XP055937803, retrieved from GENBANK Database accession no. AY302556
DATABASE NUCLEOTIDE 29 December 2003 (2003-12-29), ANONYMOUS: "Human echovirus 29 strain JV-10 complete genome", XP055937797, retrieved from GENBANK Database accession no. AY302552
DATABASE NUCLEOTIDE 29 December 2003 (2003-12-29), ANONYMOUS: "Human echovirus 33 strain Toluca-3 complete genome", XP055937803, retrieved from GENBANK Database accession no. AY302556
Attorney, Agent or Firm:
LINDA LIU & PARTNERS (CN)
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