Title:
CIS-ZEATIN O-GLCOCYLTRANSFERASE FROM ZEA MAYS
Document Type and Number:
WIPO Patent Application WO/2001/094564
Kind Code:
A2
Abstract:
Isolated nucleic acids encoding cis-zeatin O-glucosyltransferase are disclosed. These nucleic acid molecules are useful, among other things, to produce transgenic plants having modified cis-zeatin O-glucosyltransferase activity and/or modified growth and developmental patterns.
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Inventors:
MOK DAVID W S (US)
MOK MACHTELD C (US)
MARTIN RUTH C (US)
HABBEN JEFFREY E (US)
MOK MACHTELD C (US)
MARTIN RUTH C (US)
HABBEN JEFFREY E (US)
Application Number:
PCT/US2001/018406
Publication Date:
December 13, 2001
Filing Date:
June 05, 2001
Export Citation:
Assignee:
OREGON STATE (US)
PIONEER HI BRED INT (US)
MOK DAVID W S (US)
MOK MACHTELD C (US)
MARTIN RUTH C (US)
HABBEN JEFFREY E (US)
PIONEER HI BRED INT (US)
MOK DAVID W S (US)
MOK MACHTELD C (US)
MARTIN RUTH C (US)
HABBEN JEFFREY E (US)
International Classes:
C07H15/26; C07K14/415; C12N9/10; C12N15/54; C12N15/82; (IPC1-7): C12N9/00
Domestic Patent References:
| WO1999051758A1 | 1999-10-14 | |||
| WO1999006571A1 | 1999-02-11 |
Other References:
DATABASE EMBL [Online] ACCESSION NO: AQ867816, 9 November 1999 (1999-11-09) WING R.A., ET AL.: "nbeb0033M20f CUGI Rice BAC Library (EcoRI) Oryza sativa genomic clone nbeb0033M20f, genomic survey sequence." XP002194549
DATABASE EMBL [Online] ACCESSION NO: AW065903, 18 October 1999 (1999-10-18) WALBOT, V.: "687002F11.y1 687 - Early embryo from Delaware Zea mays cDNA, mRNA sequence." XP002194550
DATABASE EMBL [Online] ACCESSION NO: AU031046, 16 October 1998 (1998-10-16) SASAKI T.: "Oryza sativa (japonica cultivar-group) cDNA, partial sequence (E60679_6Z)." XP002194551
MARTIN RUTH C ET AL: "A maize cytokinin gene encoding an O-glucosyltransferase specific to cis-zeatin." PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 98, no. 10, 8 May 2001 (2001-05-08), pages 5922-5926, XP002194543 May 8, 2001 ISSN: 0027-8424 -& DATABASE EMBL [Online] ACCESSION NO: AF318075, 17 May 2001 (2001-05-17) MARTIN R.C., ET AL.: "Zea mays cis-zeatin O-glucosyltransferase (ciszog1) mRNA, complete cds." XP002194555
MARTIN RITH C ET AL: "Isolation of a cytokinin gene, ZOG1, encoding zeatin O-glucosyltransferase from Phaseolus lunatus." PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 96, no. 1, 5 January 1999 (1999-01-05), pages 284-289, XP002194544 ISSN: 0027-8424
DATABASE EMBL [Online] ACCESSION NO: AF116858, 1 September 1999 (1999-09-01) MARTIN R.C., ET AL.: "Phaseolus vulgaris zeatin O-xylosyltransferase (ZOX1) gene, complete cds." XP002194552 -& MARTIN RUTH C ET A: "A gene encoding the cytokinin enzyme zeatin O-xylosyltransferase of Phaseolus vulgaris." PLANT PHYSIOLOGY (ROCKVILLE), vol. 120, no. 2, June 1999 (1999-06), pages 553-557, XP002194554
DIXON S C ET AL: "ZEATIN GLYCOSYLATION ENZYMES IN PHASEOLUS ISOLATION OF O GLUCOSYLTRANSFERASE FROM PHASEOLUS-LUNATUS AND COMPARISON TO O XYLOSYLTRANSFERASE FROM PHASEOLUS-VULGARIS" PLANT PHYSIOLOGY (BETHESDA), vol. 90, no. 4, 1989, pages 1316-1321, XP002194545 ISSN: 0032-0889
MARTIN RUTH C ET AL: "Protein processing and auxin response in transgenic tobacco harboring a putative cDNA of zeatin O-xylosyltransferase from Phaseolus vulgaris." PLANT JOURNAL, vol. 12, no. 2, 1997, pages 305-312, XP002194546 ISSN: 0960-7412
DIETRICH J T ET AL: "ISOLATION OF STAGE-SPECIFIC TRANSCRIPTS FROM DEVELOPING MAIZE ENDOSPERM" PLANT PHYSIOLOGY (BETHESDA), vol. 99, no. 1 SUPPL., 1992, page 31 XP002194547 ISSN: 0032-0889
DATABASE BIOSIS [Online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1988 HOCART C H ET AL: "CYTOKININS OF DRY ZEA-MAYS SEED QUANTIFICATION BY RADIOIMMUNOASSAY AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY" Database accession no. PREV198886042085 XP002194553 & JOURNAL OF PLANT GROWTH REGULATION, vol. 7, no. 3, 1988, pages 179-196, ISSN: 0721-7595
SUGIYAMA T ET AL: "CYTOKININS IN DEVELOPING TUBEROUS ROOTS OF SWEET POTATO" AGRICULTURAL AND BIOLOGICAL CHEMISTRY, vol. 53, no. 1, 1989, pages 49-52, XP002194548 ISSN: 0002-1369
DATABASE EMBL [Online] ACCESSION NO: AW065903, 18 October 1999 (1999-10-18) WALBOT, V.: "687002F11.y1 687 - Early embryo from Delaware Zea mays cDNA, mRNA sequence." XP002194550
DATABASE EMBL [Online] ACCESSION NO: AU031046, 16 October 1998 (1998-10-16) SASAKI T.: "Oryza sativa (japonica cultivar-group) cDNA, partial sequence (E60679_6Z)." XP002194551
MARTIN RUTH C ET AL: "A maize cytokinin gene encoding an O-glucosyltransferase specific to cis-zeatin." PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 98, no. 10, 8 May 2001 (2001-05-08), pages 5922-5926, XP002194543 May 8, 2001 ISSN: 0027-8424 -& DATABASE EMBL [Online] ACCESSION NO: AF318075, 17 May 2001 (2001-05-17) MARTIN R.C., ET AL.: "Zea mays cis-zeatin O-glucosyltransferase (ciszog1) mRNA, complete cds." XP002194555
MARTIN RITH C ET AL: "Isolation of a cytokinin gene, ZOG1, encoding zeatin O-glucosyltransferase from Phaseolus lunatus." PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 96, no. 1, 5 January 1999 (1999-01-05), pages 284-289, XP002194544 ISSN: 0027-8424
DATABASE EMBL [Online] ACCESSION NO: AF116858, 1 September 1999 (1999-09-01) MARTIN R.C., ET AL.: "Phaseolus vulgaris zeatin O-xylosyltransferase (ZOX1) gene, complete cds." XP002194552 -& MARTIN RUTH C ET A: "A gene encoding the cytokinin enzyme zeatin O-xylosyltransferase of Phaseolus vulgaris." PLANT PHYSIOLOGY (ROCKVILLE), vol. 120, no. 2, June 1999 (1999-06), pages 553-557, XP002194554
DIXON S C ET AL: "ZEATIN GLYCOSYLATION ENZYMES IN PHASEOLUS ISOLATION OF O GLUCOSYLTRANSFERASE FROM PHASEOLUS-LUNATUS AND COMPARISON TO O XYLOSYLTRANSFERASE FROM PHASEOLUS-VULGARIS" PLANT PHYSIOLOGY (BETHESDA), vol. 90, no. 4, 1989, pages 1316-1321, XP002194545 ISSN: 0032-0889
MARTIN RUTH C ET AL: "Protein processing and auxin response in transgenic tobacco harboring a putative cDNA of zeatin O-xylosyltransferase from Phaseolus vulgaris." PLANT JOURNAL, vol. 12, no. 2, 1997, pages 305-312, XP002194546 ISSN: 0960-7412
DIETRICH J T ET AL: "ISOLATION OF STAGE-SPECIFIC TRANSCRIPTS FROM DEVELOPING MAIZE ENDOSPERM" PLANT PHYSIOLOGY (BETHESDA), vol. 99, no. 1 SUPPL., 1992, page 31 XP002194547 ISSN: 0032-0889
DATABASE BIOSIS [Online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1988 HOCART C H ET AL: "CYTOKININS OF DRY ZEA-MAYS SEED QUANTIFICATION BY RADIOIMMUNOASSAY AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY" Database accession no. PREV198886042085 XP002194553 & JOURNAL OF PLANT GROWTH REGULATION, vol. 7, no. 3, 1988, pages 179-196, ISSN: 0721-7595
SUGIYAMA T ET AL: "CYTOKININS IN DEVELOPING TUBEROUS ROOTS OF SWEET POTATO" AGRICULTURAL AND BIOLOGICAL CHEMISTRY, vol. 53, no. 1, 1989, pages 49-52, XP002194548 ISSN: 0002-1369
Attorney, Agent or Firm:
Harding, Tanya M. (Sparkman Campbell, Leigh & Whinston, LLP
One World Trade Cente, Suite 1600 121 SW Salmon Street Portland OR, US)
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Claims:
We claim:
| 1. | A purified polypeptide, comprising an amino acid sequence selected from the group consisting of : (a) the sequence set forth in SEQ ID NO: 8; (b) sequences that differ from the sequence set forth in SEQ ID NO: 8 by one or more conservative amino acid substitutions; (c) sequences that differ from the sequence set forth in SEQ ID NO: 8 by addition or deletion of one or more amino acid; and (d) sequences that share at least 45% sequence identity with the sequence set forth in SEQ ID NO: 8, wherein the polypeptide has ciszeatinOglucosyltransferase activity. |
| 2. | The purified polypeptide of claim 1, comprising a sequence that differs from the sequence set forth in SEQ ID NO: 8 by addition or deletion of 10 or more amino acids. |
| 3. | The purified polypeptide of claim 2, wherein the polypeptide is a fusion protein. |
| 4. | The purified polypeptide of claim 1, comprising an amino acid sequence that shares at least 65% sequence identity with the sequence set forth in SEQ ID NO: 8. |
| 5. | The purified polypeptide of claim 1, comprising an amino acid sequence that shares at least 75% sequence identity with the sequence set forth in SEQ ID NO: 8. |
| 6. | The purified polypeptide of claim 1, comprising an amino acid sequence that shares at least 85% sequence identity with the sequence set forth in SEQ ID NO: 8. |
| 7. | The purified polypeptide of claim 1, comprising an amino acid sequence that shares at least 95% sequence identity with the sequence set forth in SEQ ID NO: 8. |
| 8. | A nucleic acid molecule encoding a polypeptide according to claim 1. |
| 9. | A recombinant nucleic acid molecule, comprising a promoter sequence operably linked to a nucleic acid molecule according to claim 8. |
| 10. | A transgenic plant, comprising a recombinant nucleic acid molecule according to claim 9. |
| 11. | A purified polypeptide, comprising an amino acid sequence shown in SEQ ID NO: 8. |
| 12. | An isolated nucleic acid molecule that encodes a polypeptide according to claim 11. |
| 13. | A recombinant nucleic acid molecule, comprising a promoter sequence operably linked to a nucleic acid molecule according to claim 12. |
| 14. | A transgenic plant, comprising a recombinant nucleic acid molecule according to claim 13. |
| 15. | An isolated nucleic acid molecule, comprising at least 15 consecutive nucleotides of a sequence selected from the group consisting of : (a) SEQ ID NO: 7; and (b) SEQ ID NO: 11. |
| 16. | An isolated nucleic acid molecule according to claim 15, comprising at least 30 consecutive nucleotides of the selected sequence. |
| 17. | An isolated nucleic acid molecule according to claim 15, comprising at least 50 consecutive nucleotides of the selected sequence. |
| 18. | A recombinant nucleic acid molecule, comprising a promoter sequence operably linked to a nucleic acid molecule according to claim 15. |
| 19. | A recombinant nucleic acid molecule according to claim 18, wherein the nucleic acid molecule is in antisense orientation with respect to the promoter sequence. |
| 20. | A transgenic plant, comprising a recombinant nucleic acid molecule according to claim 15. |
| 21. | A transgenic plant, comprising a recombinant nucleic acid molecule according to claim 19. |
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