Title:
CO-EXPRESSION OF ZEIN PROTEINS
Document Type and Number:
WIPO Patent Application WO2002086077
Kind Code:
A3
Abstract:
Methods and constructs for transformed plants and plant tissues that are capable of expressing high levels of stable proteins localized as protein bodies within the plant cell. Transformed plants co-expressing high levels of both the 15 kD and 10 kD zein proteins are disclosed which accumulate to high levels as protein bodies in the vegetative tissue of the plant. Transformed plants co-expressing the 15 kD and 10 kD zein proteins are useful for providing forage crops containing increased levels of sulfur containing amino acids, such as methionine, in the diet of animals that normally feed on such crops. In one embodiment, a stable protein body is expressed in a plant or plant tissue as a fusion protein comprising both the 15 kD and 10 kD zein proteins operably linked by a polypeptide or peptide linker. The protein bodies provided in the present invention are resistant to rumin digestion or environmental degradation.
Inventors:
KEMP JOHN D (US)
RANDALL JENNIFER J (US)
RANDALL JENNIFER J (US)
Application Number:
PCT/US2002/012646
Publication Date:
October 21, 2004
Filing Date:
April 18, 2002
Export Citation:
Assignee:
UNIV NEW MEXICO STATE TECH TRA (US)
KEMP JOHN D (US)
RANDALL JENNIFER J (US)
KEMP JOHN D (US)
RANDALL JENNIFER J (US)
International Classes:
C07K14/425; C12N15/62; C12N15/82; (IPC1-7): C12N5/04; A01H5/00; C12N15/82
Foreign References:
| US5990384A | 1999-11-23 |
Download PDF: