Title:
COMPLEX COMPRISING RNAI MOLECULE AND N-ACETYLATED CHITOSAN
Document Type and Number:
WIPO Patent Application WO/2017/078054
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a composition that comprises an RNAi molecule which inhibits CHST15 gene expression, and that enables minimally invasive administration. Provided are the following: a complex comprising an RNAi molecule which inhibits CHST15 gene expression, and N-acetylated chitosan; a method for producing the complex comprising an RNAi molecule which inhibits CHST15 gene expression, and N-acetylated chitosan, wherein the method has (a) a step for forming the complex by mixing the RNAi molecule and chitosan, (b) a step for drying the complex obtained by step (a), and (c) a step for acetylation of the chitosan in the dry complex obtained by step (b); and a medical composition comprising the complex.
More Like This:
Inventors:
YONEYAMA HIROYUKI (JP)
Application Number:
PCT/JP2016/082545
Publication Date:
May 11, 2017
Filing Date:
November 02, 2016
Export Citation:
Assignee:
STELIC INST & CO INC (JP)
International Classes:
A61K31/713; A61K47/36; A61K48/00; A61P1/04; A61P43/00
Domestic Patent References:
| WO2014013535A1 | 2014-01-23 |
Foreign References:
| JP2014518875A | 2014-08-07 | |||
| JP2006516988A | 2006-07-13 | |||
| JPH10509207A | 1998-09-08 |
Other References:
KAI, E. ET AL.: "A Method for Oral DNA Delivery with N-Acetylated Chitosan", PHARMACEUTICAL RESEARCH, vol. 21, no. 5, 1 May 2004 (2004-05-01), pages 838 - 843, XP055541259, DOI: 10.1023/B:PHAM.0000026437.32238.6f
MAO, S. ET AL.: "Chitosan-based formulations for delivery of DNA and siRNA", ADVANCED DRUG DELIVERY REVIEWS, vol. 62, no. 1, 2010, pages 12 - 27, XP 029627554
MALMO, J. ET AL.: "siRNA delivery with chitosan nanoparticles: Molecular properties favoring efficient gene silencing", JOURNAL OF CONTROLLED RELEASE, vol. 158, no. 2, 2012, pages 261 - 268, XP 055382177
ARUMUGAM, S. ET AL.: "Orally Active siRNA Targeting Carbohydrate Sulfotransferase 15 Alleviates Colonic Mucosal Injury in Mice", GASTROENTEROLOGY, vol. 150, no. 4, April 2016 (2016-04-01), pages S-125, XP 029511769
See also references of EP 3372234A4
MAO, S. ET AL.: "Chitosan-based formulations for delivery of DNA and siRNA", ADVANCED DRUG DELIVERY REVIEWS, vol. 62, no. 1, 2010, pages 12 - 27, XP 029627554
MALMO, J. ET AL.: "siRNA delivery with chitosan nanoparticles: Molecular properties favoring efficient gene silencing", JOURNAL OF CONTROLLED RELEASE, vol. 158, no. 2, 2012, pages 261 - 268, XP 055382177
ARUMUGAM, S. ET AL.: "Orally Active siRNA Targeting Carbohydrate Sulfotransferase 15 Alleviates Colonic Mucosal Injury in Mice", GASTROENTEROLOGY, vol. 150, no. 4, April 2016 (2016-04-01), pages S-125, XP 029511769
See also references of EP 3372234A4
Attorney, Agent or Firm:
HIRAKI Yusuke et al. (JP)
Download PDF: