Title:
COMPOSITE MICROBIAL AGENT FOR DEGRADING PETROLEUM HYDROCARBONS, AND PREPARATION METHOD THEREFOR AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/039921
Kind Code:
A1
Abstract:
Provided are a composite microbial agent for degrading petroleum hydrocarbons, and a preparation method therefor and the use thereof. The composite microbial agent is composed of live Acinetobacter KJ-1 with the deposit number of CGMCC No. 20664, Providencia L1 with the deposit number of CGMCC No. 16526, Bacillus SWH-1 with the deposit number of GMCC No. 1950, and Sphingobacterium SWH-2 with the deposit number of CGMCC No. 1951. The prepared composite microbial agent for degrading petroleum hydrocarbons can effectively degrade petroleum hydrocarbon substances in soil and oil sludge, can be effectively applied to the biodegradation treatment of petroleum-contaminated soil, and does not induce secondary pollution on the ecological environment of the soil. By fully utilising the synergistic effect of live Acinetobacter KJ-1, Providencia L1, Bacillus SWH-1 and Sphingobacterium SWH-2, the final degradation efficiency of petroleum hydrocarbons is improved. The solid microbial agent has a simple preparation process which is easily operated, is low cost, and has wide application prospects in the field of bioremediation of petroleum-contaminated soil and petroleum product leakage accident sites.
Inventors:
WANG JIANING (CN)
HUANG YUJIE (CN)
HUO QIANWEI (CN)
SONG FANYONG (CN)
CHEN GUANHONG (CN)
ZHANG QIANG (CN)
FU XIAOWEN (CN)
HUANG YUJIE (CN)
HUO QIANWEI (CN)
SONG FANYONG (CN)
CHEN GUANHONG (CN)
ZHANG QIANG (CN)
FU XIAOWEN (CN)
Application Number:
PCT/CN2021/119762
Publication Date:
March 23, 2023
Filing Date:
September 23, 2021
Export Citation:
Assignee:
ECOLOGY INST OF SHANDONG ACADEMY OF SCIENCES (CN)
International Classes:
C12N1/20; B09C1/10; C12R1/01
Foreign References:
| CN110564635A | 2019-12-13 | |||
| CN112725240A | 2021-04-30 | |||
| CN101890426A | 2010-11-24 | |||
| CN109207413A | 2019-01-15 |
Other References:
YU YANG; ZHANG WEN; CHEN GUANHONG; GAO YONGCHAO; WANG JIANING: "Preparation of petroleum-degrading bacterial agent and its application in remediation of contaminated soil in Shengli Oil Field, China ", ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, SPRINGER BERLIN HEIDELBERG, BERLIN/HEIDELBERG, vol. 21, no. 13, 22 March 2014 (2014-03-22), Berlin/Heidelberg, pages 7929 - 7937, XP035314724, ISSN: 0944-1344, DOI: 10.1007/s11356-014-2707-0
LI CHAOMIN, WANG JIA-NING, QIU WEI-ZHONG, CHI JIAN-GUO: "Isolation and Identification of Highly Efficient Oil-degrading Bacteria and Study of Their Degradation Capability", BIOTECHNOLOGY, vol. 17, no. 4, 31 August 2007 (2007-08-31), pages 80 - 82, XP093048943, ISSN: 1004-311x, DOI: 10.16519/j.cnki.1004-311x.2007.04.019
LI JIA, CAO XINGTAO; SUI HONG; HE LIN; LI XINGANG: "Overview of Remediation Technologies for Petroleum-Contaminated Soils", SHIYOU XUEBAO. SHIYOU JIAGONG - ACTA PETROLEI SINICA. PETROLEUM PROCESSING SECTION, SHIYOU XUEBAO, BEIJING, CN, vol. 33, no. 5, 30 September 2017 (2017-09-30), BEIJING, CN , pages 811 - 833, XP093048942, ISSN: 1001-8719, DOI: 10.3969/j.issn.1001-8719.2017.05.002
LI CHAOMIN, WANG JIA-NING, QIU WEI-ZHONG, CHI JIAN-GUO: "Isolation and Identification of Highly Efficient Oil-degrading Bacteria and Study of Their Degradation Capability", BIOTECHNOLOGY, vol. 17, no. 4, 31 August 2007 (2007-08-31), pages 80 - 82, XP093048943, ISSN: 1004-311x, DOI: 10.16519/j.cnki.1004-311x.2007.04.019
LI JIA, CAO XINGTAO; SUI HONG; HE LIN; LI XINGANG: "Overview of Remediation Technologies for Petroleum-Contaminated Soils", SHIYOU XUEBAO. SHIYOU JIAGONG - ACTA PETROLEI SINICA. PETROLEUM PROCESSING SECTION, SHIYOU XUEBAO, BEIJING, CN, vol. 33, no. 5, 30 September 2017 (2017-09-30), BEIJING, CN , pages 811 - 833, XP093048942, ISSN: 1001-8719, DOI: 10.3969/j.issn.1001-8719.2017.05.002
Attorney, Agent or Firm:
JINAN QUANCHENG PATENT & TRADEMARK OFFICE (CN)
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