Title:
MUTANT STRAIN SELECTED USING AEROSPACE BREEDING TECHNOLOGY
Document Type and Number:
WIPO Patent Application WO/2023/039922
Kind Code:
A1
Abstract:
Provided are a mutant strain of carotenoid-producing yeast and an application of a mutation site thereof. Saccharomyces cerevisiae W2, deposited in the GuangDong Microbial Culture Collection Center, having the deposit number GDMCC No: 61335. On the basis of W2, strain W2-A-5 was obtained by further modification, and the deposit number is GDMCC No: 61337. A Saccharomyces cerevisiae mutant strain W2 is obtained by means of microgravity mutagenesis screening. The carotenoid content of the strain can reach 45mg/g cell weight under conditions of shake flask fermentation. On the basis of this strain, W2-A-5 was obtained from further modification. The carotenoid content can reach 68 mg/g dry cell weight. From genome sequencing and reverse metabolic engineering research, it was determined that CHO2 gene inactivation is the main factor for the enhanced carotenoid synthesis ability of W2. After CHO2 inactivation in strains without growth retardation, the ability to synthesize carotenoids was significantly increased, thereby providing a new target for targeted modification of Saccharomyces cerevisiae for increasing carotenoid synthesis ability.
Inventors:
ZHU HONGHUI (CN)
SU BULI (CN)
LI ANZHANG (CN)
DENG MINGRONG (CN)
FENG GUANGDA (CN)
SU BULI (CN)
LI ANZHANG (CN)
DENG MINGRONG (CN)
FENG GUANGDA (CN)
Application Number:
PCT/CN2021/119871
Publication Date:
March 23, 2023
Filing Date:
September 23, 2021
Export Citation:
Assignee:
INST OF MICROBIOLOGY GUANGDONG ACADEMY OF SCIENCES GUANGDONG DETECTION CENTER OF MICROBIOLOGY (CN)
GUANGDONG BOWOTE BIOTECHNOLOGY CO LTD (CN)
GUANGDONG BOWOTE BIOTECHNOLOGY CO LTD (CN)
International Classes:
C12N15/31; C12N1/18; C12N9/88; C12N15/01; C12N15/81; C12P23/00; C12R1/865
Foreign References:
| CN107513504A | 2017-12-26 | |||
| CN112481144A | 2021-03-12 | |||
| JPH0622748A | 1994-02-01 | |||
| US20180148728A1 | 2018-05-31 |
Other References:
HAN, LI-LI; LIU, MIN: "Application of Mutagenicity in Microorganism Breeding", LIQUOR MAKING, vol. 35, no. 3, 31 May 2008 (2008-05-31), pages 16 - 18, XP009544650, ISSN: 1002-8110
XU, NING ET AL.: "Combining protein and metabolic engineering to construct efficient microbial cell factories", CURRENT OPINION IN BIOTECHNOLOGY, vol. 66, 12 July 2020 (2020-07-12), pages 27 - 35, XP086407359, DOI: 10.1016/j.copbio.2020.06.001
XU, NING ET AL.: "Combining protein and metabolic engineering to construct efficient microbial cell factories", CURRENT OPINION IN BIOTECHNOLOGY, vol. 66, 12 July 2020 (2020-07-12), pages 27 - 35, XP086407359, DOI: 10.1016/j.copbio.2020.06.001
Attorney, Agent or Firm:
GUANGZHOU KEYUE I.P. LAW OFFICE (CN)
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