Title:
COMPOSITION FOR CLEAVING A TARGET DNA COMPRISING A GUIDE RNA SPECIFIC FOR THE TARGET DNA AND CAS PROTEIN-ENCODING NUCLEIC ACID OR CAS PROTEIN, AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2014/065596
Kind Code:
A1
Abstract:
The present invention relates to targeted genome editing in eukaryotic cells or organisms. More particularly, the present invention relates to a composition for cleaving a target DNA in eukaryotic cells or organisms comprising a guide RNA specific for the target DNA and Cas protein-encoding nucleic acid or Cas protein, and use thereof.
Inventors:
KIM JIN-SOO (KR)
CHO SEUNG WOO (KR)
KIM SOJUNG (KR)
KIM JONG MIN (KR)
KIM SEOKJOONG (KR)
CHO SEUNG WOO (KR)
KIM SOJUNG (KR)
KIM JONG MIN (KR)
KIM SEOKJOONG (KR)
Application Number:
PCT/KR2013/009488
Publication Date:
May 01, 2014
Filing Date:
October 23, 2013
Export Citation:
Assignee:
TOOLGEN INC (KR)
International Classes:
C12N15/11; C12Q1/68
Foreign References:
| US5766900A | 1998-06-16 | |||
| US5767367A | 1998-06-16 | |||
| US20100076057A1 | 2010-03-25 |
Other References:
JINEK, MARTIN ET AL.: "A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity", SCIENCE, vol. 337, no. 6096, 17 August 2012 (2012-08-17), pages 816 - 821, XP055067747
GASIUNAS, GIEDRIUS ET AL.: "Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria", PNAS, vol. 109, no. 39, 4 September 2012 (2012-09-04), pages 2579 - 2586, XP055068588
CHO, SEUNG WOO ET AL.: "Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease", NATURE BIOTECHNOLOGY, vol. 31, no. 3, 29 January 2013 (2013-01-29), pages 230 - 232, XP002699850
HSU, PATRICK D. ET AL.: "DNA targeting specificity of RNA-guided Cas9 nucleases", NATURE BIOTECHNOLOGY, vol. 31, no. 9, 21 July 2013 (2013-07-21), pages 827 - 834, XP002718604
MALI, PRASHANT ET AL.: "RNA-guided human genome engineering via Cas9", SCIENCE, vol. 339, no. 6121, 15 February 2013 (2013-02-15), pages 823 - 826, XP055161524
HWANG, WOONG Y. ET AL.: "Efficient genome editing in zebrafish using a CRISPR-Cas system", NATURE BIOTECHNOLOGY, vol. 31, no. 3, 29 January 2013 (2013-01-29), pages 1 - 3, XP055086625
See also references of EP 2912175A1
GASIUNAS, GIEDRIUS ET AL.: "Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria", PNAS, vol. 109, no. 39, 4 September 2012 (2012-09-04), pages 2579 - 2586, XP055068588
CHO, SEUNG WOO ET AL.: "Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease", NATURE BIOTECHNOLOGY, vol. 31, no. 3, 29 January 2013 (2013-01-29), pages 230 - 232, XP002699850
HSU, PATRICK D. ET AL.: "DNA targeting specificity of RNA-guided Cas9 nucleases", NATURE BIOTECHNOLOGY, vol. 31, no. 9, 21 July 2013 (2013-07-21), pages 827 - 834, XP002718604
MALI, PRASHANT ET AL.: "RNA-guided human genome engineering via Cas9", SCIENCE, vol. 339, no. 6121, 15 February 2013 (2013-02-15), pages 823 - 826, XP055161524
HWANG, WOONG Y. ET AL.: "Efficient genome editing in zebrafish using a CRISPR-Cas system", NATURE BIOTECHNOLOGY, vol. 31, no. 3, 29 January 2013 (2013-01-29), pages 1 - 3, XP055086625
See also references of EP 2912175A1
Attorney, Agent or Firm:
SON, Min (STX R&D Center 6th Floor,163, YangJaeCheon-ro, Gangnam-gu, Seoul 135-855, KR)
Download PDF:
Previous Patent: IMAGE DISPLAY DEVICE AND METHOD FOR CONTROLLING SAME
Next Patent: METHOD FOR CONTROLLING PRESS FORMING
Next Patent: METHOD FOR CONTROLLING PRESS FORMING