COMPOSITION FOR SIMULTANEOUSLY DETECTING MYCOBACTERIUM TUBERCULOSIS AND NONTUBERCULOUS MYCOBACTERIA BY MEANS OF REAL-TIME MULTIPLEX POLYMERASE CHAIN REACTION COMPRISING NESTED HYBRIDIZATION PNA PROBE SYSTEM HAVING PARALLEL BINDING STRUCTURE, AND METHOD FOR DETECTION USING SAME

WIPO Patent Application WO/2013/009084

A2

The present invention relates to a composition for simultaneously detecting Mycobacterium tuberculosis and nontuberculous mycobacteria based on a nested hybridization peptide nucleic acid (PNA) probe system having a parallel binding structure, and to a method for simultaneously detecting Mycobacterium tuberculosis and nontuberculous mycobacteria using the composition and real-time polymerase chain reaction. Also, the present invention relates to a primer, a probe composition, and a method for analyzing to simultaneously detect Mycobacterium tuberculosis and nontuberculous mycobacteria based on the nested hybridization PNA probe system having the parallel binding structure. When carrying out the real-time multiplexed polymerase chain reaction by using the composition comprising the nested hybridized PNA probe system having the parallel binding structure, according to the present invention, Mycobacterium tuberculosis and nontuberculous mycobacteria can be simultaneously and accurately detected in a single tube with high sensitivity, thereby providing the advantage of making a more rapid and accurate clinical diagnosis.

WO/2000/075307	HYGROMYCIN TOLERANCE GENE AND TRANSFORMED YEAST BELONGING TO THE GENUS CANDIDA
WO/2003/033702	METHOD OF DETECTING AND QUANTIFYING HEMOLYSIN−PRODUCING BACTERIA BY OVERWHELMINGLY DETECTING AND QUANTIFYING THERMOSTABLE HEMOLYSIN−RELATED GENES &lpar tdh−RELATED HEMOLYSIN GENES&rpar OF FOOD POISONING BACTERIA
WO/2001/005958	ANTISENSE MODULATION OF P13 KINASE P110 DELTA EXPRESSION

KIM SUNG-KEE (KR)
PARK CHWANG SIEK (KR)
JOE GOON HO (KR)
PARK HEEKYUNG (KR)
KIM JIHYUN (KR)
CHOI JI-AH (KR)
CHOI SUNG ROK (KR)
KIM SU NAM (KR)
KIM YONGTAE (KR)
KIM SE RYUN (KR)
JANG HYUNJUNG (KR)

PCT/KR2012/005483

January 17, 2013

July 11, 2012

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PANAGENE INC (KR)
KIM SUNG-KEE (KR)
PARK CHWANG SIEK (KR)
JOE GOON HO (KR)
PARK HEEKYUNG (KR)
KIM JIHYUN (KR)
CHOI JI-AH (KR)
CHOI SUNG ROK (KR)
KIM SU NAM (KR)
KIM YONGTAE (KR)
KIM SE RYUN (KR)
JANG HYUNJUNG (KR)

C12N15/11; C12Q1/04; C12Q1/68

US6355421B1

2002-03-12

STENDER, H. ET AL.: 'Fluorescence In Situ Hybridization Assay Using Peptide Nucleic Acid Probes for Differentiation between Tuberculous and Nontuberculous Mycobacterium Species in Smears of Mycobacterium Cultures' JOURNAL OF CLINICAL MICROBIOLOGY vol. 37, 30 September 1999, pages 2760 - 2765
TEDESCHI, T. ET AL.: 'Lysine Based Peptide Nucleic Acids (PNAs) with Strong Chiral Constraint: Control of Helix Handedness and DNA Binding by Chirality' CHIRALITY vol. 17, 31 December 2005, pages 196 - 204
RAY, A. ET AL.: 'Peptide nucleic acid (PNA), its medical and biotechnical applications and promise for the future' THE FASEB JOURNAL vol. 14, 30 June 2000, pages 1041 - 1060
DROBNIEWSKI, F. A. ET AL.: 'Differentiation of Mycobacterium tuberculosis Complex and Nontuberculous Mycobacterial Liquid Cultures by Using Peptide Nucleic Acid Fluorescence In Situ Hybridization Probes' JOURNAL OF CLINICAL MICROBIOLOGY vol. 38, 31 January 2000, pages 444 - 447

KWON, Oh-Sig et al. (KR)
권오식 (KR)

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