Title:
COMPOSITIONS AND METHODS FOR THE SIMULTANEOUS GENOMIC, TRANSCRIPTOMIC AND PROTEOMIC ANALYSIS OF SINGLE CELLS
Document Type and Number:
WIPO Patent Application WO/2018/089910
Kind Code:
A3
Abstract:
Disclosed are compositions and methods for the multiplexed analysis of one or more intracellular targets of a single cell. Exemplary compositions of the disclosure comprise a surface comprising a plurality of capture agents operatively-linked thereto, wherein each capture agent specifically binds to a distinct intracellular target and wherein the plurality of capture agents form a repeating pattern; a substrate comprising a plurality of chambers, wherein the substrate releasably couples with the surface and wherein each chamber of the plurality of chambers comprises at least one repeat of the repeating pattern of the plurality of capture agents of the surface; a coating composition comprising a cell lysis composition; and a linker composition comprising a functionalization component and an extension component.
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Inventors:
SRINIVASAN MAITHREYAN (US)
NG COLIN (US)
KAISER ALIANA (US)
BETTINI EMILY (US)
PACZKOWSKI PATRICK (US)
MACKAY SEAN (US)
LIU FEIMEI (US)
NG COLIN (US)
KAISER ALIANA (US)
BETTINI EMILY (US)
PACZKOWSKI PATRICK (US)
MACKAY SEAN (US)
LIU FEIMEI (US)
Application Number:
PCT/US2017/061344
Publication Date:
November 22, 2018
Filing Date:
November 13, 2017
Export Citation:
Assignee:
ISOPLEXIS CORP (US)
International Classes:
G01N33/543; C12N15/11; C12N15/115; C12Q1/68; G01N33/68
Domestic Patent References:
| WO2013090404A2 | 2013-06-20 | |||
| WO2016057552A1 | 2016-04-14 |
Other References:
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Q. SHI ET AL: "Single-cell proteomic chip for profiling intracellular signaling pathways in single tumor cells", PROCEEDINGS NATIONAL ACADEMY OF SCIENCES PNAS, vol. 109, no. 2, 27 December 2011 (2011-12-27), US, pages 419 - 424, XP055410819, ISSN: 0027-8424, DOI: 10.1073/pnas.1110865109
ANIRUDDH SARKAR ET AL: "Microfluidic probe for single-cell analysis in adherent tissue culture", NATURE COMMUNICATIONS, vol. 5, 5 March 2014 (2014-03-05), United Kingdom, XP055382538, ISSN: 2041-1723, DOI: 10.1038/ncomms4421
YOUNG SHIK SHIN ET AL: "Chemistries for Patterning Robust DNA MicroBarcodes Enable Multiplex Assays of Cytoplasm Proteins from Single Cancer Cells", CHEMPHYSCHEM, vol. 11, no. 14, 4 October 2010 (2010-10-04), pages 3063 - 3069, XP055136895, ISSN: 1439-4235, DOI: 10.1002/cphc.201000528
H SEDGWICK ET AL: "Lab-on-a-chip technologies for proteomic analysis from isolated cells", JOURNAL OF THE ROYAL SOCIETY. INTERFACE, vol. 5, no. Suppl_2, 6 October 2008 (2008-10-06), GB, pages S123 - S130, XP055444609, ISSN: 1742-5689, DOI: 10.1098/rsif.2008.0169.focus
K. EYER ET AL: "Implementing Enzyme-Linked Immunosorbent Assays on a Microfluidic Chip To Quantify Intracellular Molecules in Single Cells", ANALYTICAL CHEMISTRY, vol. 85, no. 6, 19 March 2013 (2013-03-19), US, pages 3280 - 3287, XP055318559, ISSN: 0003-2700, DOI: 10.1021/ac303628j
"On-Chip Pretreatment of Whole Blood by Using MEMS Technology", 31 July 2012, BENTHAM SCIENCE PUBLISHERS, ISBN: 978-1-60805-147-2, article XING CHEN ET AL: "Microfluidic Devices Targeting Blood Cell Lysis", pages: 64 - 83, XP055444364, DOI: 10.2174/978160805147211201010064
YANG YU ET AL: "Analysis of the surface, secreted, and intracellular proteome of Propionibacterium acnes", EUPA OPEN PROTEONOMICS, vol. 9, 1 December 2015 (2015-12-01), NL, pages 1 - 7, XP055444597, ISSN: 2212-9685, DOI: 10.1016/j.euprot.2015.06.003
Q. SHI ET AL: "Single-cell proteomic chip for profiling intracellular signaling pathways in single tumor cells", PROCEEDINGS NATIONAL ACADEMY OF SCIENCES PNAS, vol. 109, no. 2, 27 December 2011 (2011-12-27), US, pages 419 - 424, XP055410819, ISSN: 0027-8424, DOI: 10.1073/pnas.1110865109
ANIRUDDH SARKAR ET AL: "Microfluidic probe for single-cell analysis in adherent tissue culture", NATURE COMMUNICATIONS, vol. 5, 5 March 2014 (2014-03-05), United Kingdom, XP055382538, ISSN: 2041-1723, DOI: 10.1038/ncomms4421
YOUNG SHIK SHIN ET AL: "Chemistries for Patterning Robust DNA MicroBarcodes Enable Multiplex Assays of Cytoplasm Proteins from Single Cancer Cells", CHEMPHYSCHEM, vol. 11, no. 14, 4 October 2010 (2010-10-04), pages 3063 - 3069, XP055136895, ISSN: 1439-4235, DOI: 10.1002/cphc.201000528
H SEDGWICK ET AL: "Lab-on-a-chip technologies for proteomic analysis from isolated cells", JOURNAL OF THE ROYAL SOCIETY. INTERFACE, vol. 5, no. Suppl_2, 6 October 2008 (2008-10-06), GB, pages S123 - S130, XP055444609, ISSN: 1742-5689, DOI: 10.1098/rsif.2008.0169.focus
Attorney, Agent or Firm:
ELRIFI, Ivor R. et al. (US)
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