Title:
CONSTRUCTION METHOD FOR GENE LIBRARY AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2019/195975
Kind Code:
A1
Abstract:
A construction method for a gene library, a gene library constructed according to the method, and a use in the field of pregnant women non-invasive prenatal testing. The construction method for a gene library comprises: co-hatching a biological sample and heparinase to obtain a co-hatching sample comprising the biological sample and the heparinase; and acquiring DNA from the co-hatching sample and performing high-throughput library construction and sequencing, wherein DNA amplification is performed using Taq enzyme in the library construction process.
Inventors:
ZHAO JIA (CN)
YANG LIN (CN)
LU SEN (CN)
PU DANDAN (CN)
GAO JIAN (CN)
GAO YA (CN)
CHEN FANG (CN)
YANG LIN (CN)
LU SEN (CN)
PU DANDAN (CN)
GAO JIAN (CN)
GAO YA (CN)
CHEN FANG (CN)
Application Number:
PCT/CN2018/082282
Publication Date:
October 17, 2019
Filing Date:
April 09, 2018
Export Citation:
Assignee:
BGI SHENZHEN (CN)
International Classes:
C40B50/06; C12Q1/68; C40B40/08
Foreign References:
| CN101466722A | 2009-06-24 | |||
| CN103403183B | 2014-10-15 | |||
| CN101613760A | 2009-12-30 | |||
| CN102186995A | 2011-09-14 |
Other References:
GROMMINGER S., ET AL.: "The influence of low molecular weight heparin me- dication on plasma DNA in pregnant women", PRENATAL DIAGNOSIS, 31 December 2015 (2015-12-31), XP055643102
Attorney, Agent or Firm:
TSINGYIHUA INTELLECTUAL PROPERTY LLC (CN)
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