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Title:
CRYOPRESERVATION PREPARATION FOR CORNEAL ENDOTHELIAL CELLS AND METHOD FOR PRODUCING SAID CRYOPRESERVATION PREPARATION
Document Type and Number:
WIPO Patent Application WO/2023/085369
Kind Code:
A1
Abstract:
The present disclosure provides a method for cryopreserving corneal endothelial cells and/or corneal-endothelial-like cells, and a freezing preparation for corneal endothelial cells and/or corneal-endothelial-like cells. The present disclosure provides a method for preserving corneal endothelial cells and/or corneal-endothelial-like cells, wherein the method includes: a freezing step for freezing unfrozen corneal endothelial cells and/or corneal-endothelial-like cells, the freezing step including at least one stage in which the temperature is reduced at a rate of less than 1°C/min when the temperature is changed from the unfrozen temperature to the freezing target temperature; and, if necessary, a step for maintaining the corneal endothelial cells and/or corneal-endothelial-like cells in a frozen state.

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Inventors:
KOIZUMI NORIKO (JP)
OKUMURA NAOKI (JP)
MATSUOKA YASUSHI (JP)
Application Number:
PCT/JP2022/041960
Publication Date:
May 19, 2023
Filing Date:
November 10, 2022
Export Citation:
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Assignee:
THE DOSHISHA (JP)
ACTUALEYES INC (JP)
International Classes:
C12N5/071; A61K35/30; A61P27/02; C12N1/04
Domestic Patent References:
WO2020045642A12020-03-05
WO2020166711A12020-08-20
WO2014142038A12014-09-18
Foreign References:
JP2019024325A2019-02-21
JP2020202772A2020-12-24
JP2018533377A2018-11-15
JP2020162533A2020-10-08
Other References:
OKUMURA NAOKI, KAGAMI TAKATO, WATANABE KYOKO, KADOYA SAORI, SATO MASAKAZU, KOIZUMI NORIKO: "Feasibility of a cryopreservation of cultured human corneal endothelial cells", PLOS ONE, vol. 14, no. 6, 21 June 2019 (2019-06-21), pages e0218431, XP093066079, DOI: 10.1371/journal.pone.0218431
Attorney, Agent or Firm:
YAMAMOTO, Shusaku et al. (JP)
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