Title:
CULTURING TISSUE USING MAGNETICALLY GENERATED MECHANICAL STRESSES
Document Type and Number:
WIPO Patent Application WO2002051985
Kind Code:
A3
Abstract:
A method of culturing tissue comprises growing tissue forming cells whilst subjecting the tissue forming cells to mechanical stresses which are generated magnetically.
Inventors:
EL-HAJ ALICIA JENNIFER HAFEEZA (GB)
DOBSON JON PAUL (GB)
DOBSON JON PAUL (GB)
Application Number:
PCT/GB2001/005606
Publication Date:
January 16, 2003
Filing Date:
December 19, 2001
Export Citation:
Assignee:
UNIV KEELE (GB)
EL-HAJ ALICIA JENNIFER HAFEEZA (GB)
DOBSON JON PAUL (GB)
EL-HAJ ALICIA JENNIFER HAFEEZA (GB)
DOBSON JON PAUL (GB)
International Classes:
C12N5/07; C12M3/00; C12N5/00; C12N5/077; (IPC1-7): C12N5/08; C12M3/00
Foreign References:
| US5486457A | 1996-01-23 |
Other References:
YUGE LOUIS ET AL: "Differentiation of myoblasts is accelerated in culture in a magnetic field.", IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY ANIMAL, vol. 36, no. 6, June 2000 (2000-06-01), pages 383 - 386, XP001061490, ISSN: 1071-2690
LIU MINGYAO ET AL: "Bio-stretch, a computerized cell strain apparatus for three-dimensional organotypic cultures.", IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY ANIMAL, vol. 35, no. 2, February 1999 (1999-02-01), pages 87 - 93, XP001080069, ISSN: 1071-2690
KASPAR D ET AL: "Dynamic cell stretching increases human osteoblast proliferation and CICP synthesis but decreases osteocalcin synthesis and alkaline phosphatase activity.", JOURNAL OF BIOMECHANICS, vol. 33, no. 1, January 2000 (2000-01-01), pages 45 - 51, XP002203098, ISSN: 0021-9290
GLOGAUER MICHAEL ET AL: "A new method for application of force to cells via ferric oxide beads.", PFLUEGERS ARCHIV EUROPEAN JOURNAL OF PHYSIOLOGY, vol. 435, no. 2, January 1998 (1998-01-01), pages 320 - 327, XP002203099, ISSN: 0031-6768
SMALT R ET AL: "Induction of NO and prostaglandin E2 in osteoblasts by wall-shear stress but not mechanical strain.", AMERICAN JOURNAL OF PHYSIOLOGY, vol. 273, no. 4 PART 1, October 1997 (1997-10-01), pages E751 - E758, XP002203100, ISSN: 0002-9513
WANG NING ET AL: "Contribution of intermediate filaments to cell stiffness, stiffening, and growth.", AMERICAN JOURNAL OF PHYSIOLOGY, vol. 279, no. 1 Part 1, July 2000 (2000-07-01), pages C188 - C194, XP002203101, ISSN: 0002-9513
WANG NING: "Mechanical interactions among cytoskeletal filaments.", HYPERTENSION (DALLAS), vol. 32, no. 1, July 1998 (1998-07-01), pages 162 - 165, XP002203102, ISSN: 0194-911X
LIU MINGYAO ET AL: "Bio-stretch, a computerized cell strain apparatus for three-dimensional organotypic cultures.", IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY ANIMAL, vol. 35, no. 2, February 1999 (1999-02-01), pages 87 - 93, XP001080069, ISSN: 1071-2690
KASPAR D ET AL: "Dynamic cell stretching increases human osteoblast proliferation and CICP synthesis but decreases osteocalcin synthesis and alkaline phosphatase activity.", JOURNAL OF BIOMECHANICS, vol. 33, no. 1, January 2000 (2000-01-01), pages 45 - 51, XP002203098, ISSN: 0021-9290
GLOGAUER MICHAEL ET AL: "A new method for application of force to cells via ferric oxide beads.", PFLUEGERS ARCHIV EUROPEAN JOURNAL OF PHYSIOLOGY, vol. 435, no. 2, January 1998 (1998-01-01), pages 320 - 327, XP002203099, ISSN: 0031-6768
SMALT R ET AL: "Induction of NO and prostaglandin E2 in osteoblasts by wall-shear stress but not mechanical strain.", AMERICAN JOURNAL OF PHYSIOLOGY, vol. 273, no. 4 PART 1, October 1997 (1997-10-01), pages E751 - E758, XP002203100, ISSN: 0002-9513
WANG NING ET AL: "Contribution of intermediate filaments to cell stiffness, stiffening, and growth.", AMERICAN JOURNAL OF PHYSIOLOGY, vol. 279, no. 1 Part 1, July 2000 (2000-07-01), pages C188 - C194, XP002203101, ISSN: 0002-9513
WANG NING: "Mechanical interactions among cytoskeletal filaments.", HYPERTENSION (DALLAS), vol. 32, no. 1, July 1998 (1998-07-01), pages 162 - 165, XP002203102, ISSN: 0194-911X
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