Title:
D-SERINE DEHYDRATASE AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2008/047802
Kind Code:
A1
Abstract:
Disclosed are: a novel D-serine quantification method which can overcome various disadvantages of a conventional D-serine quantification method; a novel enzyme which can be used in the D-serine quantification method; a gene encoding the enzyme; and others. Specifically disclosed are: a novel D-serine dehydratase which comprises (a) a protein having the amino acid sequence depicted in SEQ ID NO:1 or (b) a protein having an amino acid sequence homologous to the amino acid sequence depicted in SEQ ID NO:1 and having a D-serine dehydratase activity; and a D-serine quantification method comprising the steps of reacting a sample with the enzyme, quantifying ammonia or pyruvic acid produced by the reaction, and calculating the amount of D-serine in the sample based on a value produced by the quantification.
Inventors:
YOSHIMURA TOHRU (JP)
ITO TOMOKAZU (JP)
ITO TOMOKAZU (JP)
Application Number:
PCT/JP2007/070193
Publication Date:
April 24, 2008
Filing Date:
October 16, 2007
Export Citation:
Assignee:
UNIV NAGOYA NAT UNIV CORP (JP)
YOSHIMURA TOHRU (JP)
ITO TOMOKAZU (JP)
YOSHIMURA TOHRU (JP)
ITO TOMOKAZU (JP)
International Classes:
C12N15/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/88; C12N15/09; C12Q1/527
Foreign References:
| JPS5811024B2 | 1983-03-01 | |||
| JPS6138463A | 1986-02-24 | |||
| JPS6144351A | 1986-03-04 | |||
| JPS5023699A | 1975-03-13 | |||
| JPS6214799A | 1987-01-23 | |||
| JPH04346796A | 1992-12-02 | |||
| JPH0595798A | 1993-04-20 | |||
| JPH05219991A | 1993-08-31 |
Other References:
DATABASE GENBANK [online] XP008115338, Database accession no. (NC_001139)
LARSSON M. ET AL.: "High-throughput protein expression of cDNA products as a tool in functional genomics", J. BIOTECHNOL., vol. 80, no. 2, 2000, pages 143 - 157, XP004213504
MISAWA K. ET AL.: "A method to identify cDNAs based on localization", PROC. NATL. ACAD. SCI. U.S.A., vol. 97, no. 7, 2000, pages 3062 - 3066, XP008109748
SAWASAKI T. ET AL.: "A cell-free protein synthesis system for high-throughput proteomics", PROC. NATL. ACAD. SCI. U.S.A., vol. 99, no. 23, 2002, pages 14652 - 14657, XP002979938
LESSON, P.D.; IVERSON, L.L., J. MED. CHEM., vol. 37, 1994, pages 4053 - 4067
HASHIMOTO ET AL., ARCH. GEN. PSYCHIATRY, vol. 60, 2003, pages 572
HASHIMOTO ET AL., PROG NEUROPSYCHOPHARMACOL BIOL PSYCHIATRY, vol. 29, 2005, pages 767
HASHIMOTO ET AL., PROG NEUROPSYCHOPHARMACOL BIOL PSYCHIATRY, vol. 28, 2004, pages 385 - 8
"Sensitive Determination of D-Amino Acids in Mammals and the Effect of D-Amino-Acid Oxidase Activity on Their Amounts", BIOLOGICAL & PHARMACEUTICAL BULLETIN, vol. 28, 2005, pages 1578
"Characterization of the catalytic pathway for D-serine dehydratase. Evidence for variation of the rate-determining step with substrate structure", J BIOL CHEM., vol. 258, no. 9, 1983, pages 5379 - 5385
"D-serine dehydratase acting also on L-serine from Klebsiella pneumoniae", J BIOCHEM., vol. 84, no. 5, November 1978 (1978-11-01), pages 1133 - 1138
KARLIN; ALTSCHUL, PROC. NATL. ACAD. SCI. USA, vol. 87, 1990, pages 2264 - 68
KARLIN; ALTSCHUL, PROC. NATL. ACAD. SCI. USA, vol. 90, 1993, pages 5873 - 77
ALTSCHUL ET AL., J. MOL. BIOL., vol. 215, 1990, pages 403 - 10
ALTSCHUL ET AL., AMINO ACIDS RESEARCH, vol. 25, no. 17, 1997, pages 3389 - 3402
MEYERS; MILLER, COMPUT. APPL. BIOSCI., vol. 4, 1988, pages 11 - 17
"Molecular Cloning", COLD SPRING HARBOR LABORATORY PRESS
"Current protocols in molecular biology", 1987
POTTER, H. ET AL., PROC. NATL. ACAD. SCI. U.S.A., vol. 81, 1984, pages 7161 - 7165
FELGNER, P.L. ET AL., PROC. NATL. ACAD. SCI. U.S.A., vol. 84, 1984, pages 7413 - 7417
GRAESSMANN, M.; GRAESSMANN, A., PROC. NATL. ACAD. SCI. U.S.A., vol. 73, 1976, pages 366 - 370
OSHIMA ET AL.: "Post-Sequence Protein Experimentation 2", 2002, TOKYO KAGAKU DOJIN CO., LTD., article "Preparation Method of Sample"
JOHN, C ET AL., BIOCHEMICAL J., vol. 159, 1976, pages 803 - 806
J. BERTHELOT: REPORT CHEM APPT 1, vol. 284, pages 1859
NISHIMURA ET AL.: "Biochemistry", vol. 30, 1991, YAKUJI NIPPO, LTD., article "In vitro diagnostic agents", pages: 4032 - 4077
TORU YOSHIMURA, VITAMIN, vol. 79, 2005, pages 277 - 283
ARIAS C.A. ET AL., MICROBIOLOGY, vol. 146, 2000, pages 1727 - 1734
OSHIMA ET AL.: "Preparation Method of Sample", 2002, TOKYO KAGAKU DOJIN CO., LTD., article "Post-Sequence Protein Experimentation 2"
TANIZAWA K. ET AL., BIOCHEMISTRY, vol. 27, 1988, pages 1311 - 1316
LIM YH ET AL., J BACTERIOL., vol. 175, 1993, pages 4213 - 4217
See also references of EP 2085472A4
LARSSON M. ET AL.: "High-throughput protein expression of cDNA products as a tool in functional genomics", J. BIOTECHNOL., vol. 80, no. 2, 2000, pages 143 - 157, XP004213504
MISAWA K. ET AL.: "A method to identify cDNAs based on localization", PROC. NATL. ACAD. SCI. U.S.A., vol. 97, no. 7, 2000, pages 3062 - 3066, XP008109748
SAWASAKI T. ET AL.: "A cell-free protein synthesis system for high-throughput proteomics", PROC. NATL. ACAD. SCI. U.S.A., vol. 99, no. 23, 2002, pages 14652 - 14657, XP002979938
LESSON, P.D.; IVERSON, L.L., J. MED. CHEM., vol. 37, 1994, pages 4053 - 4067
HASHIMOTO ET AL., ARCH. GEN. PSYCHIATRY, vol. 60, 2003, pages 572
HASHIMOTO ET AL., PROG NEUROPSYCHOPHARMACOL BIOL PSYCHIATRY, vol. 29, 2005, pages 767
HASHIMOTO ET AL., PROG NEUROPSYCHOPHARMACOL BIOL PSYCHIATRY, vol. 28, 2004, pages 385 - 8
"Sensitive Determination of D-Amino Acids in Mammals and the Effect of D-Amino-Acid Oxidase Activity on Their Amounts", BIOLOGICAL & PHARMACEUTICAL BULLETIN, vol. 28, 2005, pages 1578
"Characterization of the catalytic pathway for D-serine dehydratase. Evidence for variation of the rate-determining step with substrate structure", J BIOL CHEM., vol. 258, no. 9, 1983, pages 5379 - 5385
"D-serine dehydratase acting also on L-serine from Klebsiella pneumoniae", J BIOCHEM., vol. 84, no. 5, November 1978 (1978-11-01), pages 1133 - 1138
KARLIN; ALTSCHUL, PROC. NATL. ACAD. SCI. USA, vol. 87, 1990, pages 2264 - 68
KARLIN; ALTSCHUL, PROC. NATL. ACAD. SCI. USA, vol. 90, 1993, pages 5873 - 77
ALTSCHUL ET AL., J. MOL. BIOL., vol. 215, 1990, pages 403 - 10
ALTSCHUL ET AL., AMINO ACIDS RESEARCH, vol. 25, no. 17, 1997, pages 3389 - 3402
MEYERS; MILLER, COMPUT. APPL. BIOSCI., vol. 4, 1988, pages 11 - 17
"Molecular Cloning", COLD SPRING HARBOR LABORATORY PRESS
"Current protocols in molecular biology", 1987
POTTER, H. ET AL., PROC. NATL. ACAD. SCI. U.S.A., vol. 81, 1984, pages 7161 - 7165
FELGNER, P.L. ET AL., PROC. NATL. ACAD. SCI. U.S.A., vol. 84, 1984, pages 7413 - 7417
GRAESSMANN, M.; GRAESSMANN, A., PROC. NATL. ACAD. SCI. U.S.A., vol. 73, 1976, pages 366 - 370
OSHIMA ET AL.: "Post-Sequence Protein Experimentation 2", 2002, TOKYO KAGAKU DOJIN CO., LTD., article "Preparation Method of Sample"
JOHN, C ET AL., BIOCHEMICAL J., vol. 159, 1976, pages 803 - 806
J. BERTHELOT: REPORT CHEM APPT 1, vol. 284, pages 1859
NISHIMURA ET AL.: "Biochemistry", vol. 30, 1991, YAKUJI NIPPO, LTD., article "In vitro diagnostic agents", pages: 4032 - 4077
TORU YOSHIMURA, VITAMIN, vol. 79, 2005, pages 277 - 283
ARIAS C.A. ET AL., MICROBIOLOGY, vol. 146, 2000, pages 1727 - 1734
OSHIMA ET AL.: "Preparation Method of Sample", 2002, TOKYO KAGAKU DOJIN CO., LTD., article "Post-Sequence Protein Experimentation 2"
TANIZAWA K. ET AL., BIOCHEMISTRY, vol. 27, 1988, pages 1311 - 1316
LIM YH ET AL., J BACTERIOL., vol. 175, 1993, pages 4213 - 4217
See also references of EP 2085472A4
Attorney, Agent or Firm:
KONISHI, Tomimasa et al. (17-12 Marunouchi 2-chome, Naka-k, Nagoya-shi Aichi 02, JP)
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