Title:
DECM EXTRACTED FROM HUMAN LUNG TISSUE AND LUNG ORGANOID CONSTRUCTION METHOD USING SAME
Document Type and Number:
WIPO Patent Application WO/2022/114937
Kind Code:
A1
Abstract:
The present invention relates to a dECM extracted from a human lung tissue and a lung organoid construction method using same. Leading to the present invention, intensive and thorough research, conducted by the present inventors, into the construction of a lung organoid for implantation into human bodies by using type 2 pneumocytes, resulted in designing a method for preparing dECM from a lung tissue and constructing a lung organoid by co-culturing the prepared dECM and type 2 pneumocytes.
Inventors:
KIM SUNG WON (KR)
JO AYOUNG (KR)
LIM MIN JAE (KR)
JO AYOUNG (KR)
LIM MIN JAE (KR)
Application Number:
PCT/KR2021/095106
Publication Date:
June 02, 2022
Filing Date:
November 19, 2021
Export Citation:
Assignee:
CATHOLIC UNIV KOREA IND ACADEMIC COOPERATION FOUNDATION (KR)
International Classes:
C12N5/071
Foreign References:
| KR20190143703A | 2019-12-31 |
Other References:
MENDIBIL UNAI, RUIZ-HERNANDEZ RAQUEL, RETEGI-CARRION SUGOI, GARCIA-URQUIA NEREA, OLALDE-GRAELLS BEATRIZ, ABARRATEGI ANDER: "Tissue-Specific Decellularization Methods: Rationale and Strategies to Achieve Regenerative Compounds", INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 21, no. 15, pages 1 - 29, XP055935177, DOI: 10.3390/ijms21155447
SHANNON, J.M. ; MASON, R.J. ; JENNINGS, S.D.: "Functional differentiation of alveolar type II epithelial cells in vitro: Effects of cell shape, cell-matrix interactions and cell-cell interactions", BIOCHIMICA ET BIOPHYSICA ACTA, vol. 931, no. 2, 12 November 1987 (1987-11-12), NL , pages 143 - 156, XP025401875, ISSN: 0167-4889, DOI: 10.1016/0167-4889(87)90200-X
LIAO DANYING, LI HUAIBIAO: "Dissecting the Niche for Alveolar Type II Cells With Alveolar Organoids", FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY, vol. 8, no. 419, pages 1 - 6, XP055935192, DOI: 10.3389/fcell.2020.00419
GIOVANNI GIUSEPPE GIOBBE, CLAIRE CROWLEY, CAMILLA LUNI, SARA CAMPINOTI, MOUSTAFA KHEDR, KAI KRETZSCHMAR, MARTINA MARIA DE SANTIS, : "Extracellular matrix hydrogel derived from decellularized tissues enables endodermal organoid culture", NATURE COMMUNICATIONS, vol. 10, no. 1, 1 December 2019 (2019-12-01), pages 1 - 14, XP055703258, DOI: 10.1038/s41467-019-13605-4
SHANNON, J.M. ; MASON, R.J. ; JENNINGS, S.D.: "Functional differentiation of alveolar type II epithelial cells in vitro: Effects of cell shape, cell-matrix interactions and cell-cell interactions", BIOCHIMICA ET BIOPHYSICA ACTA, vol. 931, no. 2, 12 November 1987 (1987-11-12), NL , pages 143 - 156, XP025401875, ISSN: 0167-4889, DOI: 10.1016/0167-4889(87)90200-X
LIAO DANYING, LI HUAIBIAO: "Dissecting the Niche for Alveolar Type II Cells With Alveolar Organoids", FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY, vol. 8, no. 419, pages 1 - 6, XP055935192, DOI: 10.3389/fcell.2020.00419
GIOVANNI GIUSEPPE GIOBBE, CLAIRE CROWLEY, CAMILLA LUNI, SARA CAMPINOTI, MOUSTAFA KHEDR, KAI KRETZSCHMAR, MARTINA MARIA DE SANTIS, : "Extracellular matrix hydrogel derived from decellularized tissues enables endodermal organoid culture", NATURE COMMUNICATIONS, vol. 10, no. 1, 1 December 2019 (2019-12-01), pages 1 - 14, XP055703258, DOI: 10.1038/s41467-019-13605-4
Attorney, Agent or Firm:
LEE, Myoung-Jin (KR)
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