Title:
DEER-DERIVED MARKER PEPTIDE SEGMENT AND DETECTION METHOD THEREFOR
Document Type and Number:
WIPO Patent Application WO/2022/048048
Kind Code:
A1
Abstract:
A deer-derived marker peptide segment and a detection method therefor. By means of experiment screening, the ratio of relative contents of two deer-derived peptide segments is studied and determined, and a graph is drawn by using the proportion of a deer horn gelatin in a mixed gelatin as an abscissa and using a value of A peptide 1/A peptide 2 as an ordinate; the proportion of the deer horn gelatin is linear with A peptide 1/A peptide 2; a standard curve equation is established as y=4.7903x+0.4106, so as to distinguish a deer skin gelatin from the deer horn gelatin. The method is strong in specificity, high in sensitivity, and simple in operation, and can be used for distinguishing the deer skin gelatin from the deer horn gelatin, and controlling the quality. There is a defect that the deer horn gelatin and the deer skin gelatin are difficult to distinguish in appearance, and are also difficult to distinguish by using a specific peptide segment. Quality control of drugs, health care foods, healthy foods, and the like containing the deer skin gelatin or the deer horn gelatin has great significance.
Inventors:
LIU RUI (CN)
CAI SHUO (CN)
JIANG MENGTONG (CN)
ZHAO KEXUAN (CN)
DUAN JINAO (CN)
CAI SHUO (CN)
JIANG MENGTONG (CN)
ZHAO KEXUAN (CN)
DUAN JINAO (CN)
Application Number:
PCT/CN2020/131797
Publication Date:
March 10, 2022
Filing Date:
November 26, 2020
Export Citation:
Assignee:
UNIV NANJING CHINESE MEDICINE (CN)
International Classes:
G01N30/88; G01N30/02
Foreign References:
| CN109187783A | 2019-01-11 | |||
| CN109187835A | 2019-01-11 |
Other References:
ZHANG, TAO ET AL.: "nano Lc-MS/MS (Investigation on Identifying Collagen from Colla Corii Bovis Using Nano LC-MS/MS Method", JOURNAL OF NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE, vol. 36, no. 1, 31 January 2020 (2020-01-31), pages 105 - 109, XP055906667, DOI: 10.14148/j.issn.1672-0482.2020.0105
LIU, RUI ET AL.: "Comparative Analysis of Chemical Constituents of Deer Hide and Deer Hide Gelatin by ''Peptidomics-Modifications'' Methods", ACTA PHARMACEUTICA SINICA, vol. 55, no. 8, 3 April 2020 (2020-04-03), pages 1882 - 1888, XP055906674, DOI: 10.16438/j.0513-4870.2020-0081
BUCKLEY, M.: "A molecular phylogeny of Plesiorycteropus reassigns the extinct mammalian order 'Bibymalagasia", PLOS ONE, vol. 8, no. 3, 26 March 2013 (2013-03-26), XP055906677, DOI: 10.1371/journal.pone.0059614
BUCKLEY, M.: "Species identification of bovine, ovine and porcine type 1 collagen; comparing peptide mass fingerprinting and LC-based proteomics method s", INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 17, 31 December 2016 (2016-12-31), pages 1 - 17, XP055606923, DOI: 10.3390/ijms17040445
LIU, RUI ET AL.: "Comparative Analysis of Chemical Constituents of Deer Hide and Deer Hide Gelatin by ''Peptidomics-Modifications'' Methods", ACTA PHARMACEUTICA SINICA, vol. 55, no. 8, 3 April 2020 (2020-04-03), pages 1882 - 1888, XP055906674, DOI: 10.16438/j.0513-4870.2020-0081
BUCKLEY, M.: "A molecular phylogeny of Plesiorycteropus reassigns the extinct mammalian order 'Bibymalagasia", PLOS ONE, vol. 8, no. 3, 26 March 2013 (2013-03-26), XP055906677, DOI: 10.1371/journal.pone.0059614
BUCKLEY, M.: "Species identification of bovine, ovine and porcine type 1 collagen; comparing peptide mass fingerprinting and LC-based proteomics method s", INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 17, 31 December 2016 (2016-12-31), pages 1 - 17, XP055606923, DOI: 10.3390/ijms17040445
Attorney, Agent or Firm:
JIANGSU SUNDY LAW FIRM (CN)
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