Title:
DETECTION OF ANTIBACTERIAL ACTIVITY IN EXCRETORY SECRETORY PRODUCT OF ADULT TRICHURIS SUIS
Document Type and Number:
WIPO Patent Application WO2002062836
Kind Code:
A3
Abstract:
The present invention provides a heat-stable and protease-resistant antibacterial activity in excretory-secretory products (ESP) of Trichuris suis. The antibacterial activity is not more than 10,000 MW; is resistant to boiling, trypsin, and pronase E; has a bactericidal mode of action; and is effective against Gram positive and Gram negative bacteria, including Escherichia coli, Campylobacter jejuni, Campylobactercoli, and Staphylococcus aureus. The antibacterial activity is useful in applications for killing or inhibiting the growth of microorganisms, in particular bacteria.
Inventors:
MANSFIELD LINDA S (US)
ABNER SHEILA R (US)
ABNER SHEILA R (US)
Application Number:
PCT/US2001/044070
Publication Date:
July 31, 2003
Filing Date:
November 06, 2001
Export Citation:
Assignee:
UNIV MICHIGAN STATE (US)
MANSFIELD LINDA S (US)
ABNER SHEILA R (US)
MANSFIELD LINDA S (US)
ABNER SHEILA R (US)
International Classes:
A01N63/12; A61K35/62; A61K38/17; C07K14/435; C12Q1/18; (IPC1-7): C07K14/435; C12Q1/18; A61K38/17
Other References:
ABNER S.R. ET AL.: "Trichuris suis: detection of antibacterilal activity in excretory-secretory products from adults", EXPERIMENTAL PARASITOLOGY, vol. 99, 2001, pages 26 - 36, XP002222863
HILL D.E. ET AL.: "Trichuris suis: thiol protease activity from adult worms", EXPERIMENTAL PARASITOLOGY, vol. 85, 1997, pages 55 - 62, XP001119012
HILL D.E. ET AL.: "Trichuris suis: a zinc metalloprotease from culture fluids of adult parasites", EXPERIMENTAL PARASITOLOGY, vol. 77, 1993, pages 170 - 178, XP002222966
ABNER S.R. ET AL.: "Trichuris suis excretory-secretory products inhibit the growth of Campylobacter jejuni", ABSTRACTS OF THE GENERAL MEETING OF THE AMERICAN SOCIETY FOR MICROBIOLOGY, vol. 99, 1999, pages 221, XP009001972
DRAKE L. ET AL: "The major secreted product of the whipworm, Trichuris, is a pore forming protein", PROC. R. SOC. LOND. B., vol. 257, 1994, pages 255 - 261, XP009001964
ABNER S.R. ET AL.: "Response of intestinal epithelial cels to Trichuris suis excretory-secretory products and the influence on campylobacter jejuni invasion under in vitro conditions", JOURNAL OF PARASITOLOGY, vol. 88, no. 4, 2002, pages 738 - 745, XP009001965
HILL D.E. ET AL.: "Trichuris suis: thiol protease activity from adult worms", EXPERIMENTAL PARASITOLOGY, vol. 85, 1997, pages 55 - 62, XP001119012
HILL D.E. ET AL.: "Trichuris suis: a zinc metalloprotease from culture fluids of adult parasites", EXPERIMENTAL PARASITOLOGY, vol. 77, 1993, pages 170 - 178, XP002222966
ABNER S.R. ET AL.: "Trichuris suis excretory-secretory products inhibit the growth of Campylobacter jejuni", ABSTRACTS OF THE GENERAL MEETING OF THE AMERICAN SOCIETY FOR MICROBIOLOGY, vol. 99, 1999, pages 221, XP009001972
DRAKE L. ET AL: "The major secreted product of the whipworm, Trichuris, is a pore forming protein", PROC. R. SOC. LOND. B., vol. 257, 1994, pages 255 - 261, XP009001964
ABNER S.R. ET AL.: "Response of intestinal epithelial cels to Trichuris suis excretory-secretory products and the influence on campylobacter jejuni invasion under in vitro conditions", JOURNAL OF PARASITOLOGY, vol. 88, no. 4, 2002, pages 738 - 745, XP009001965
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