Title:
DEVICE FOR MAPPING SEQUENCING DATA IN GENETIC SCREENING, AND COMPUTER PROGRAM STORED IN MEDIUM TO IMPLEMENT EACH FUNCTION IN DEVICE
Document Type and Number:
WIPO Patent Application WO/2023/146025
Kind Code:
A1
Abstract:
The present invention relates to a method (technique) for mapping sequencing data in genetic screening, which enables sequence mapping to be implemented in a novel method involving: predicting a data format by means of unsupervised learning based on data characteristics of the sequencing data in genetic screening; and, on the basis of the data format, dynamically identifying/extracting a library sequence for the sequence mapping.
Inventors:
MUN JI HYEOB (KR)
LIM BYUNG HO (KR)
LIM BYUNG HO (KR)
Application Number:
PCT/KR2022/006625
Publication Date:
August 03, 2023
Filing Date:
May 10, 2022
Export Citation:
Assignee:
KOREA INST SCI & TECH INF (KR)
KOREA RES INST CHEMICAL TECH (KR)
KOREA RES INST CHEMICAL TECH (KR)
International Classes:
G16B30/10; C12Q1/686; C12Q1/6869; G16B25/20; G16B35/20
Foreign References:
| KR20180016888A | 2018-02-20 |
Other References:
SCHAEFER CHRISTIANE, MALLELA NIKHIL, SEGGEWISS JOCHEN, LECHTAPE BIRGIT, OMRAN HEYMUT, DIRKSEN UTA, KORSCHING EBERHARD, POTRATZ JEN: "Target discovery screens using pooled shRNA libraries and next-generation sequencing: A model workflow and analytical algorithm", PLOS ONE, vol. 13, no. 1, 31 January 2018 (2018-01-31), pages e0191570 - e0191570-23, XP093081999, DOI: 10.1371/journal.pone.0191570
LIN JIAXING, GRESHAM JEREMY, WANG TONGRONG, KIM SO YOUNG, ALVAREZ JAMES, DAMRAUER JEFFREY S, FLOYD SCOTT, GRANEK JOSHUA, ALLEN AND: "bcSeq: an R package for fast sequence mapping in high-throughput shRNA and CRISPR screens", BIOINFORMATICS, OXFORD UNIVERSITY PRESS , SURREY, GB, vol. 34, no. 20, 15 October 2018 (2018-10-15), GB , pages 3581 - 3583, XP093082004, ISSN: 1367-4803, DOI: 10.1093/bioinformatics/bty402
HUANG XIAODONG; WANG JEAN YJ; LU XIN: "Systems analysis of quantitative shRNA-library screens identifies regulators of cell adhesion", BMC SYSTEMS BIOLOGY, BIOMED CENTRAL LTD, LO, vol. 2, no. 1, 13 June 2008 (2008-06-13), Lo , pages 49, XP021040102, ISSN: 1752-0509
KAMPMANN MARTIN, BASSIK MICHAEL C, WEISSMAN JONATHAN S: "Functional genomics platform for pooled screening and generation of mammalian genetic interaction maps", NATURE PROTOCOLS, NATURE PUBLISHING GROUP, GB, vol. 9, no. 8, 1 August 2014 (2014-08-01), GB , pages 1825 - 1847, XP093082006, ISSN: 1754-2189, DOI: 10.1038/nprot.2014.103
포스터 발표 (Poster Presentation). MUN, Jihyeob et al. The method for enhanced mapping of genome-wide shRNA screen data based on next-generation sequencing. International Conference KSMCB 2021. 03-05 November 2021.
LIN JIAXING, GRESHAM JEREMY, WANG TONGRONG, KIM SO YOUNG, ALVAREZ JAMES, DAMRAUER JEFFREY S, FLOYD SCOTT, GRANEK JOSHUA, ALLEN AND: "bcSeq: an R package for fast sequence mapping in high-throughput shRNA and CRISPR screens", BIOINFORMATICS, OXFORD UNIVERSITY PRESS , SURREY, GB, vol. 34, no. 20, 15 October 2018 (2018-10-15), GB , pages 3581 - 3583, XP093082004, ISSN: 1367-4803, DOI: 10.1093/bioinformatics/bty402
HUANG XIAODONG; WANG JEAN YJ; LU XIN: "Systems analysis of quantitative shRNA-library screens identifies regulators of cell adhesion", BMC SYSTEMS BIOLOGY, BIOMED CENTRAL LTD, LO, vol. 2, no. 1, 13 June 2008 (2008-06-13), Lo , pages 49, XP021040102, ISSN: 1752-0509
KAMPMANN MARTIN, BASSIK MICHAEL C, WEISSMAN JONATHAN S: "Functional genomics platform for pooled screening and generation of mammalian genetic interaction maps", NATURE PROTOCOLS, NATURE PUBLISHING GROUP, GB, vol. 9, no. 8, 1 August 2014 (2014-08-01), GB , pages 1825 - 1847, XP093082006, ISSN: 1754-2189, DOI: 10.1038/nprot.2014.103
포스터 발표 (Poster Presentation). MUN, Jihyeob et al. The method for enhanced mapping of genome-wide shRNA screen data based on next-generation sequencing. International Conference KSMCB 2021. 03-05 November 2021.
Attorney, Agent or Firm:
NAM & NAM (KR)
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