Title:
DIFFERENTIATED CELLS
Document Type and Number:
WIPO Patent Application WO2005059119
Kind Code:
A3
Abstract:
The adult hair follicle dermal papilla and dermal sheath cell are developmentally active cell populations with a proven role in adult hair follicle cycling activity and unique inductive powers. In stein cell biology, the hair follicle epithelium has recently been the subject of a great deal of investigation, but up to now the follicle dermis has been largely overlooked as a source of stein cells. Following the sporadic appearance of muscle, lipid and bone-type cells in discretely isolated follicle dermal papilla and sheath cell primary cultures, we demonstrated that cultured papilla and sheath cell lines were capable of being directed to lipid and bone differentiation. Subsequently, for the First time, we produced clonal dermal papilla and sheath lines that had extended proliferative capabilities. Dye exclusion has been reported to be an identifying feature of stein cells, therefore clonal papilla and sheath lines with differing capacity to exclude rhodamine (123) were cultured in medium known to induce adipocyte and osteocyte differentiation. Both dermal sheath and dermal papilla-derived clones showed the capacity to make lipid and to produce calcified material, however different clones had varied behaviour and there was no obvious correlation between their stein cell capabilities, and dye exclusion or selected gene expression markers. As a highly accessible source, capable of being discretely isolated, the follicle has important potential as a stein cell source for tissue engineering and cell therapy purposes. It will also be interesting to compare follicle dermal stein cell properties with the broader stein cell capabilities discovered in skin dermis, and investigate whether the follicle is a key dermal stein cell niche. Finally, the discovery of stein cells in the dermis may have implications for certain pathologies in which abnormal differentiation occurs in the skin.
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Inventors:
JAHODA COLIN ALBERT BUCHANAN (GB)
REYNOLDS AMANDA JANE (GB)
WHITEHOUSE CLAIRE JENNA (GB)
HOLE NICHOLAS (GB)
REYNOLDS AMANDA JANE (GB)
WHITEHOUSE CLAIRE JENNA (GB)
HOLE NICHOLAS (GB)
Application Number:
PCT/GB2004/005232
Publication Date:
August 11, 2005
Filing Date:
December 14, 2004
Export Citation:
Assignee:
UNIV DURHAM (GB)
JAHODA COLIN ALBERT BUCHANAN (GB)
REYNOLDS AMANDA JANE (GB)
WHITEHOUSE CLAIRE JENNA (GB)
HOLE NICHOLAS (GB)
JAHODA COLIN ALBERT BUCHANAN (GB)
REYNOLDS AMANDA JANE (GB)
WHITEHOUSE CLAIRE JENNA (GB)
HOLE NICHOLAS (GB)
International Classes:
C12N5/071; C12N5/077; A61K35/12; (IPC1-7): C12N5/06; A61K35/12; A61K121/00
Domestic Patent References:
| WO2003104443A2 | 2003-12-18 |
Foreign References:
| US5851831A | 1998-12-22 |
Other References:
JAHODA COLIN A B ET AL: "Hair follicle dermal cells differentiate into adipogenic and osteogenic lineages.", EXPERIMENTAL DERMATOLOGY. DEC 2003, vol. 12, no. 6, December 2003 (2003-12-01), pages 849 - 859, XP002326457, ISSN: 0906-6705
LAKO M ET AL: "Hair follicle dermal cells repopulate the mouse haematopoietic system", JOURNAL OF CELL SCIENCE, CAMBRIDGE UNIVERSITY PRESS, LONDON, GB, vol. 115, no. Pt 20, 15 October 2002 (2002-10-15), pages 3967 - 3974, XP002296955, ISSN: 0021-9533
LAKO L ET AL: "Cells of the dermal sheath and dermal papilla possess haematopoietic potential", DEVELOPMENT GROWTH AND DIFFERENTIATION, JAPANESE SOCIETY OF DEVELOPMENTAL BIOLOGISTS,, JP, vol. 43, no. Supplement, July 2001 (2001-07-01), pages S22, XP002296954, ISSN: 0012-1592
ROBINSON MARK ET AL: "In vivo induction of hair growth by dermal cells isolated from hair follicles after extended organ culture", JOURNAL OF INVESTIGATIVE DERMATOLOGY, vol. 117, no. 3, September 2001 (2001-09-01), pages 596 - 604, XP002326458, ISSN: 0022-202X
GHARZI A ET AL: "Plasticity of hair follicle dermal cells in wound healing and induction.", EXPERIMENTAL DERMATOLOGY. APR 2003, vol. 12, no. 2, April 2003 (2003-04-01), pages 126 - 136, XP002326459, ISSN: 0906-6705
JAHODA C A ET AL: "Hair follicle dermal sheath cells: unsung participants in wound healing", LANCET THE, LANCET LIMITED. LONDON, GB, vol. 358, no. 9291, 27 October 2001 (2001-10-27), pages 1445 - 1448, XP004806066, ISSN: 0140-6736
IGUCHI MAKIKO ET AL: "Human follicular papilla cells carry out nonadipose tissue production of leptin", JOURNAL OF INVESTIGATIVE DERMATOLOGY, vol. 117, no. 6, December 2001 (2001-12-01), pages 1349 - 1356, XP002326576, ISSN: 0022-202X
LAKO M ET AL: "Hair follicle dermal cells repopulate the mouse haematopoietic system", JOURNAL OF CELL SCIENCE, CAMBRIDGE UNIVERSITY PRESS, LONDON, GB, vol. 115, no. Pt 20, 15 October 2002 (2002-10-15), pages 3967 - 3974, XP002296955, ISSN: 0021-9533
LAKO L ET AL: "Cells of the dermal sheath and dermal papilla possess haematopoietic potential", DEVELOPMENT GROWTH AND DIFFERENTIATION, JAPANESE SOCIETY OF DEVELOPMENTAL BIOLOGISTS,, JP, vol. 43, no. Supplement, July 2001 (2001-07-01), pages S22, XP002296954, ISSN: 0012-1592
ROBINSON MARK ET AL: "In vivo induction of hair growth by dermal cells isolated from hair follicles after extended organ culture", JOURNAL OF INVESTIGATIVE DERMATOLOGY, vol. 117, no. 3, September 2001 (2001-09-01), pages 596 - 604, XP002326458, ISSN: 0022-202X
GHARZI A ET AL: "Plasticity of hair follicle dermal cells in wound healing and induction.", EXPERIMENTAL DERMATOLOGY. APR 2003, vol. 12, no. 2, April 2003 (2003-04-01), pages 126 - 136, XP002326459, ISSN: 0906-6705
JAHODA C A ET AL: "Hair follicle dermal sheath cells: unsung participants in wound healing", LANCET THE, LANCET LIMITED. LONDON, GB, vol. 358, no. 9291, 27 October 2001 (2001-10-27), pages 1445 - 1448, XP004806066, ISSN: 0140-6736
IGUCHI MAKIKO ET AL: "Human follicular papilla cells carry out nonadipose tissue production of leptin", JOURNAL OF INVESTIGATIVE DERMATOLOGY, vol. 117, no. 6, December 2001 (2001-12-01), pages 1349 - 1356, XP002326576, ISSN: 0022-202X
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