Title:
DOUBLE-KNOCKOUT NON-HUMAN MODEL ANIMAL FOR RNA INTERFERENCE
Document Type and Number:
WIPO Patent Application WO/2007/102516
Kind Code:
A1
Abstract:
The object is to provide a double-knockout non-human animal which can cause RNA interference with double-stranded RNA in an mammal and use of the animal. A double-knockout non-human animal lacking the functions of RIG-I and MDA5 which are normally expressed in a wild-type one is prepared by deleting a gene encoding RIG-I and a gene encoding MDA5 or causing the mutation in these genes, and is used for RNA interference. The non-human model animal can cause RNA interference induced by long double-stranded RNA having a 30-nucleotide length or longer.
Inventors:
AKIRA SHIZUO (JP)
KATO HIROKI (JP)
TAKEUCHI OSAMU (JP)
KATO HIROKI (JP)
TAKEUCHI OSAMU (JP)
Application Number:
PCT/JP2007/054354
Publication Date:
September 13, 2007
Filing Date:
March 06, 2007
Export Citation:
Assignee:
JAPAN SCIENCE & TECH AGENCY (JP)
UNIV OSAKA (JP)
AKIRA SHIZUO (JP)
KATO HIROKI (JP)
TAKEUCHI OSAMU (JP)
UNIV OSAKA (JP)
AKIRA SHIZUO (JP)
KATO HIROKI (JP)
TAKEUCHI OSAMU (JP)
International Classes:
C12N15/00; A01K67/027; C12N15/09; C12Q1/68
Foreign References:
| JP2003144141A | 2003-05-20 |
Other References:
KATO H. ET AL.: "Differential roles of MDA5 and RIG-I helicases in the recognition of RNA viruses", NATURE, vol. 441, no. 7089, 4 May 2006 (2006-05-04), pages 101 - 105, XP003017391
YONEYAMA M. ET AL.: "The RNA helicase RIG-I has an essential function in double-stranded RNA-induced innate antiviral responses", NAT. IMMUNOL., vol. 5, no. 7, July 2004 (2004-07-01), pages 730 - 737, XP003017392
KATO H. ET AL.: "Cell type-specific involvement of RIG-I in antiviral response", IMMUNITY, vol. 23, no. 1, July 2005 (2005-07-01), pages 19 - 28, XP003017393
KOVACSOVICS M. ET AL.: "Overexpression of Helicard, a CARD-containing helicase cleaved during apoptosis, accelerates DNA degradation", CURR. BIOL., vol. 12, no. 10, 14 May 2002 (2002-05-14), pages 838 - 843, XP003017394
ANDREJEVA J. ET AL.: "The V proteins of paramyxoviruses bind te IFN-inducible RNA helicase, mda-5, and inhibit its activation of the IFN-beta promoter", PROC. NATL. ACAD. SCI. U.S.A., vol. 101, no. 49, 7 December 2004 (2004-12-07), pages 17264 - 17269, XP003017395
YONEYAMA M. ET AL.: "The RNA helicase RIG-I has an essential function in double-stranded RNA-induced innate antiviral responses", NAT. IMMUNOL., vol. 5, no. 7, July 2004 (2004-07-01), pages 730 - 737, XP003017392
KATO H. ET AL.: "Cell type-specific involvement of RIG-I in antiviral response", IMMUNITY, vol. 23, no. 1, July 2005 (2005-07-01), pages 19 - 28, XP003017393
KOVACSOVICS M. ET AL.: "Overexpression of Helicard, a CARD-containing helicase cleaved during apoptosis, accelerates DNA degradation", CURR. BIOL., vol. 12, no. 10, 14 May 2002 (2002-05-14), pages 838 - 843, XP003017394
ANDREJEVA J. ET AL.: "The V proteins of paramyxoviruses bind te IFN-inducible RNA helicase, mda-5, and inhibit its activation of the IFN-beta promoter", PROC. NATL. ACAD. SCI. U.S.A., vol. 101, no. 49, 7 December 2004 (2004-12-07), pages 17264 - 17269, XP003017395
Attorney, Agent or Firm:
HIROTA, Masanori (8-5 Akasaka 2-chome, Minato-k, Tokyo 52, JP)
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