Title:
DRY POWDER CELLS AND CELL CULTURE REAGENTS AND METHODS OF PRODUCTION THEREOF
Document Type and Number:
WIPO Patent Application WO2002036735
Kind Code:
A3
Abstract:
The present invention relates generally to nutritive (e.g., cell culture) medium, medium supplement, media subgroup and buffer formulations. Specifically, the present invention provides powdered nutritive medium, medium supplement and medium subgroup formulations, particularly cell culture medium supplements (including powdered sera such as powdered fetal bovine seum (FBS)), medium subgroup formulations and cell culture media comprising necessary nutritive factors that facilitate the in vitro cultivation of cells. The invention further provides such powdered formulations that produce particular or desired final ionic and/or pH conditions upon reconstitution with a solvent. The invention si also directed to methods of roduction of these formulations, and also provides kits and methods for cultivation of prokaryotic and eukaryotic cells using these formulations. The invention also relates to methods of producing sterile formulations, and to methods for producing dry cell powders. The invention also provides cell, media, media supplement, media subgroup and buffer powders produced by the methods of the invention.
Inventors:
FIKE RICHARD MILTON
HASSETT RICHARD FRANCIS
DADEY BARBARA MARY
RADOMINSKI ROBERT CHESTER
HASSETT RICHARD FRANCIS
DADEY BARBARA MARY
RADOMINSKI ROBERT CHESTER
Application Number:
PCT/US2001/042982
Publication Date:
June 20, 2002
Filing Date:
November 06, 2001
Export Citation:
Assignee:
INVITROGEN CORP (US)
International Classes:
C12N1/00; C12N5/00; C12N5/02; (IPC1-7): C12N5/00; C12N5/02; C12N5/04; C12N5/06
Domestic Patent References:
| WO1994027645A1 | 1994-12-08 |
Foreign References:
| US4071412A | 1978-01-31 | |||
| US4615978A | 1986-10-07 | |||
| US5474931A | 1995-12-12 |
Other References:
See also references of EP 1339829A4
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