Title:
ENGINEERING PROTEIN POSTTRANSLATIONAL MODIFICATION IN TRANSGENIC ORGANISMS
Document Type and Number:
WIPO Patent Application WO1996034966
Kind Code:
A3
Abstract:
The invention relates to transgenic non-human multicellular organisms that contain polynucleotides for expressing proteins that alter posttranslational modification. In particular, the invention provides multiply-transgenic animals in which a first transgene encodes a first protein, a second transgene encodes a second protein, and expression of the second protein affects the posttranlational modification of the first protein in cells of said organism. Expression in preferred embodiments is in specific cells and the modified protein is secreted into a bodily fluid. The invention provides related methods, proteins and products. An example provides transgenic animals that express human Protein C and the processing protease PACE/furin in mammary glands and secrete both proteins into milk.
Inventors:
LUBON HENRYK
DROHAN WILLIAM N
PALEYANDA REKHA K
DROHAN WILLIAM N
PALEYANDA REKHA K
Application Number:
PCT/US1996/006121
Publication Date:
December 05, 1996
Filing Date:
May 06, 1996
Export Citation:
Assignee:
AMERICAN NAT RED CROSS (US)
International Classes:
C07K14/75; C12N9/64; C12N15/64; C12N15/67; C12N15/82; C12N15/85; A61K38/00; (IPC1-7): C12N15/64; A01K67/027; C12N9/64; C12N15/67; C12N15/82; C12N15/85
Domestic Patent References:
| WO1992009698A1 | 1992-06-11 | |||
| WO1995017514A1 | 1995-06-29 |
Foreign References:
| EP0319944A2 | 1989-06-14 |
Other References:
LOUISE C WASLEY ET AL.: "PACE/Furin can process the vitamin k-dependent pro-factor IX precursor within the secretory pathway.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 268, no. 12, 25 April 1993 (1993-04-25), MD US, pages 8458 - 8465, XP002014347
D.C.FOSTER ET AL.: "Endoproteolytic processing of the human protein C precursor by the yeast Kex2 endopeptidase coexpressed in mammalian cells.", BIOCHEMISTRY, vol. 30, 1991, EASTON, PA US, pages 367 - 372, XP002014348
DREWS, ROMAN ET AL: "Proteolytic maturation of protein C upon engineering the mouse mammary gland to express furin", PROC. NATL. ACAD. SCI. U. S. A. (1995), 92(23), 10462-6 CODEN: PNASA6;ISSN: 0027-8424, 1995, XP002014349
D.C.FOSTER ET AL.: "Endoproteolytic processing of the human protein C precursor by the yeast Kex2 endopeptidase coexpressed in mammalian cells.", BIOCHEMISTRY, vol. 30, 1991, EASTON, PA US, pages 367 - 372, XP002014348
DREWS, ROMAN ET AL: "Proteolytic maturation of protein C upon engineering the mouse mammary gland to express furin", PROC. NATL. ACAD. SCI. U. S. A. (1995), 92(23), 10462-6 CODEN: PNASA6;ISSN: 0027-8424, 1995, XP002014349
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