Title:
ENZYME EXHIBITING α-1,6-GLUCOSYL TRANSFER ACTIVITY
Document Type and Number:
WIPO Patent Application WO/2019/163777
Kind Code:
A1
Abstract:
The present invention pertains to: an enzyme that shows α-1,6-glucosyl transfer activity, is capable of utilizing a partial degraded product of starch as a substrate, exhibits heat resistance, and is suitable for industrial application; an α-1,6-glucan production enzyme agent which contains said enzyme as an active ingredient; and an α-1,6-glucan production method using said enzyme or enzyme agent. The present invention provides an enzyme which shows α-1,6-glucosyl transfer activity and which is any one protein selected from: (a) a protein having the amino acid sequence described in SEQ ID NO:3; (b) a protein having an amino acid sequence having at least 90% amino acid sequence identity with the amino acid sequence described in SEQ ID NO:3; and (c) a protein having an amino acid sequence obtained by substituting, inserting, deleting, and/or adding one or more amino acid residues in the amino acid sequence described in SEQ ID NO:3.
Inventors:
MORI HARUHIDE (JP)
SABURI WATARU (JP)
KANAI KENTA (JP)
AIZAWA KENTA (JP)
IIZUKA TAKAHISA (JP)
TAKECHI NORIAKI (JP)
TANI MIOKA (JP)
SABURI WATARU (JP)
KANAI KENTA (JP)
AIZAWA KENTA (JP)
IIZUKA TAKAHISA (JP)
TAKECHI NORIAKI (JP)
TANI MIOKA (JP)
Application Number:
PCT/JP2019/006111
Publication Date:
August 29, 2019
Filing Date:
February 19, 2019
Export Citation:
Assignee:
UNIV HOKKAIDO NAT UNIV CORP (JP)
JAPAN MAIZE PROD (JP)
JAPAN MAIZE PROD (JP)
International Classes:
C12N9/10; A23K20/163; C08B37/00; C12P19/18
Other References:
DATABASE GenBank [online] 8 May 2019 (2019-05-08), "glycosyl hydrolase, glucoamylase [Thermoanaerobacter siderophilus SR4", Database accession no. EIV99888.1
MISHIMA, ERI ET AL.: "Analysis of the transglycosylation exhibited by the GH15 domain-containing enzyme that is derived from Thermoanaerobacter Siderophilus", BULLETIN OF APPLIED GLYCOSCIENCE, ''JOURNAL OF APPLIED GLYCOSCIENCE, vol. 7, 20 August 2017 (2017-08-20)
MISHIMA, ERI ET AL.: "Analysis of the transglycosylation exhibited by the GH15 domain-containing enzyme that is derived from Thermoanaerobacter Siderophilus", BULLETIN OF APPLIED GLYCOSCIENCE, ''JOURNAL OF APPLIED GLYCOSCIENCE, vol. 7, 20 August 2017 (2017-08-20)
Attorney, Agent or Firm:
SIKS & CO. (JP)
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