Title:
ETHANOL PRODUCTION BY TRANSFORMATION OF GRAM-POSITIVE MICROBES
Document Type and Number:
WIPO Patent Application WO/1995/027064
Kind Code:
A1
Abstract:
The subject invention concerns the transformation of Gram-positive bacteria with heterologous genes which confer upon these microbes the ability to produce ethanol as a fermentation product. Specifically exemplified is the transformation of bacteria with genes, obtainable from Zymomonas mobilis, which encode pyruvate decarboxylase and alcohol dehydrogenase.
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Inventors:
INGRAM LONNIE O'NEAL
BARBOSA-ALLEYNE MARIA DE F S
BARBOSA-ALLEYNE MARIA DE F S
Application Number:
PCT/US1995/004012
Publication Date:
October 12, 1995
Filing Date:
March 30, 1995
Export Citation:
Assignee:
UNIV FLORIDA (US)
International Classes:
C12M1/02; C12N1/21; C12N9/04; C12N9/24; C12N9/88; C12N15/01; C12N15/52; C12N15/53; C12N15/70; C12N15/74; C12N15/90; C12P7/06; C12P7/10; G06T1/00; (IPC1-7): C12N15/53; C12N1/21; C12N15/56; C12N15/60; C12N15/75; C12P7/06
Domestic Patent References:
| WO1992016615A1 | 1992-10-01 | |||
| WO1990002193A1 | 1990-03-08 |
Other References:
B.E. WOOD ET AL: "Ethanol production from cellobiose, amorphous cellulose and critalline cellulose by recombinant Klebsiella oxytoca containing chromosomally integrated Zymomonas mobilis genes for ethanol production and plasmids expressing thermostable cellulase genes from Clostridium thermocellum", APPLIED AND ENVIRONMENT MICROBIOLOGY, vol. 58, no. 7, July 1992 (1992-07-01), pages 2103 - 2110
MARIA DE F.S. BARBOSA ET AL: "Expression of the Zymomonas mobilis alcohol dehydrogenase II (adhB) and pyruvate decarboxylase (pdc) genes in Bacillus", CURRENT MICROBIOLOGY, vol. 28, no. 5, May 1994 (1994-05-01), pages 279 - 282
R.S GOLD ET AL: "Expression of the Zymomonas mobilis "production of ethanol " genes by Lactobacillus casei", ABSTRACTS OF THE GENERAL MEETING OF THE AMERICAN SOCIETY FOR MICROBIOLOGY, vol. 95, no. 0, May 1995 (1995-05-01), pages 380
MARIA DE F.S. BARBOSA ET AL: "Expression of the Zymomonas mobilis alcohol dehydrogenase II (adhB) and pyruvate decarboxylase (pdc) genes in Bacillus", CURRENT MICROBIOLOGY, vol. 28, no. 5, May 1994 (1994-05-01), pages 279 - 282
R.S GOLD ET AL: "Expression of the Zymomonas mobilis "production of ethanol " genes by Lactobacillus casei", ABSTRACTS OF THE GENERAL MEETING OF THE AMERICAN SOCIETY FOR MICROBIOLOGY, vol. 95, no. 0, May 1995 (1995-05-01), pages 380
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