Title:
Fc-BINDING PROTEIN, METHOD FOR PRODUCING SAID PROTEIN, AND ANTIBODY ADSORBENT USING SAID PROTEIN, AND METHODS FOR PURIFYING AND IDENTIFYING ANTIBODY USING SAID ADSORBENT
Document Type and Number:
WIPO Patent Application WO/2015/041303
Kind Code:
A1
Abstract:
The present invention addresses the first problem of providing an Fc-binding protein having improved stability, especially stability to heat and acid, of the Fc-binding protein, a method for producing this protein, and an antibody adsorbent using this protein. The present invention also addresses the second problem of providing a method that makes it possible to identify the presence or absence of glycosylation of an antibody, and a material to be used in this method. The first problem is solved by an Fc-binding protein having improved stability to heat and acid obtained by substituting amino acid residues at specific positions in the extracellular domain within human FcγRIIIa with other specific amino acids, a method for producing this protein, and an antibody adsorbent using this protein. The second problem is solved by using an adsorbent capable of specifically adsorbing an antibody having a sugar chain, the adsorbent being obtained by immobilizing human FcγRIIIa on an insoluble carrier.
Inventors:
ASAOKA YOSHIHARU (JP)
TANAKA TORU (JP)
IDE TERUHIKO (JP)
TANAKA TORU (JP)
IDE TERUHIKO (JP)
Application Number:
PCT/JP2014/074739
Publication Date:
March 26, 2015
Filing Date:
September 18, 2014
Export Citation:
Assignee:
TOSOH CORP (JP)
International Classes:
C07K14/735; C07K1/22; C12N1/21; C12N15/09; C12P21/02
Domestic Patent References:
| WO2000032767A1 | 2000-06-08 | |||
| WO1996034953A2 | 1996-11-07 |
Foreign References:
| JP2011097898A | 2011-05-19 | |||
| JP2011206046A | 2011-10-20 |
Other References:
ROGERS, K.A. ET AL.: "IgG Fc receptor III homologues in nonhuman primate species: genetic characterization and ligand interactions.", J. IMMUNOL., vol. 177, no. 6, 15 September 2006 (2006-09-15), pages 3848 - 3856, XP055248767
ZOU, G. ET AL.: "Chemoenzymatic synthesis and Fcy receptor binding of homogeneous glycoforms of antibody Fc domain. Presence of a bisecting sugar moiety enhances the affinity of Fc to FcyIIIa receptor.", J. AM. CHEM. SOC., vol. 133, no. 46, 23 November 2011 (2011-11-23), pages 18975 - 18991, XP055061687
SHIBATA-KOYAMA, M. ET AL.: "The N-linked oligosaccharide at Fc gamma RIIIa Asn-45: an inhibitory element for high Fc gamma RIIIa binding affinity to IgG glycoforms lacking core fucosylation.", GLYCOBIOLOGY, vol. 19, no. 2, February 2009 (2009-02-01), pages 126 - 134, XP055332309
ELLSWORTH, J.L. ET AL.: "Generation of a high- affinity Fcgamma receptor by Ig-domain swapping between human CD 64A and CD 16A.", PROTEIN ENG. DES. SEL., vol. 23, no. 4, April 2010 (2010-04-01), pages 299 - 309, XP055332310
FERRARA, C. ET AL.: "The carbohydrate at FcgammaRIIIa Asn-162. An element required for high affinity binding to non-fucosylated IgG glycoforms.", J. BIOL. CHEM., vol. 281, no. 8, 24 February 2006 (2006-02-24), pages 5032 - 5036, XP002430982
See also references of EP 3048112A4
TAKAI.T., JPN. J. CLIN. IMMUNOL., vol. 28, 2005, pages 318 - 326
J. GALON ET AL., EUR. J. IMMUNOL., vol. 27, 1997, pages 1928 - 1932
SHINKAWA, T., J. BIOL. CHEM., vol. 278, 2003, pages 3466 - 3473
TAKAI, T., JPN. J. CLIN. IMMUNOL., vol. 28, 2005, pages 318 - 326
"Structure and Function of Protein", vol. 9, 2005, MEDICAL SCIENCE INTERNATIONAL, INC.
"Molecular Cloning", 1992, COLD SPRING HARBOR LABORATORY, pages: 256
NUCLEIC ACIDS RES., vol. 30, 2002, pages E43
ZOU, G. ET AL.: "Chemoenzymatic synthesis and Fcy receptor binding of homogeneous glycoforms of antibody Fc domain. Presence of a bisecting sugar moiety enhances the affinity of Fc to FcyIIIa receptor.", J. AM. CHEM. SOC., vol. 133, no. 46, 23 November 2011 (2011-11-23), pages 18975 - 18991, XP055061687
SHIBATA-KOYAMA, M. ET AL.: "The N-linked oligosaccharide at Fc gamma RIIIa Asn-45: an inhibitory element for high Fc gamma RIIIa binding affinity to IgG glycoforms lacking core fucosylation.", GLYCOBIOLOGY, vol. 19, no. 2, February 2009 (2009-02-01), pages 126 - 134, XP055332309
ELLSWORTH, J.L. ET AL.: "Generation of a high- affinity Fcgamma receptor by Ig-domain swapping between human CD 64A and CD 16A.", PROTEIN ENG. DES. SEL., vol. 23, no. 4, April 2010 (2010-04-01), pages 299 - 309, XP055332310
FERRARA, C. ET AL.: "The carbohydrate at FcgammaRIIIa Asn-162. An element required for high affinity binding to non-fucosylated IgG glycoforms.", J. BIOL. CHEM., vol. 281, no. 8, 24 February 2006 (2006-02-24), pages 5032 - 5036, XP002430982
See also references of EP 3048112A4
TAKAI.T., JPN. J. CLIN. IMMUNOL., vol. 28, 2005, pages 318 - 326
J. GALON ET AL., EUR. J. IMMUNOL., vol. 27, 1997, pages 1928 - 1932
SHINKAWA, T., J. BIOL. CHEM., vol. 278, 2003, pages 3466 - 3473
TAKAI, T., JPN. J. CLIN. IMMUNOL., vol. 28, 2005, pages 318 - 326
"Structure and Function of Protein", vol. 9, 2005, MEDICAL SCIENCE INTERNATIONAL, INC.
"Molecular Cloning", 1992, COLD SPRING HARBOR LABORATORY, pages: 256
NUCLEIC ACIDS RES., vol. 30, 2002, pages E43
Attorney, Agent or Firm:
AOKI, Atsushi et al. (JP)
Aoki 篤 (JP)
Aoki 篤 (JP)
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