Title:
Fc-INTERFERON-BETA FUSION PROTEINS
Document Type and Number:
WIPO Patent Application WO2006000448
Kind Code:
A3
Abstract:
Disclosed are Fc-interferon-beta (Fc-IFN-ß) fusion proteins and nucleic acid molecules encoding them. The Fc- IFN-ß fusion proteins include variants of the interferon-beta (IFN-ß) protein that are altered to achieve enhanced biological activity, prolonged circulating half-life and greater solubility. Also disclosed are methods of producing the fusion proteins and methods of using the fusion proteins and/or nucleic acid molecules for treating diseases and conditions alleviated by the administration of interferon-beta.
Inventors:
GILLIES STEPHEN D (US)
WATKINS NIGEL JOHN (GB)
BAKER MATTHEW PAUL (GB)
LO KING-MING (US)
WATKINS NIGEL JOHN (GB)
BAKER MATTHEW PAUL (GB)
LO KING-MING (US)
Application Number:
PCT/EP2005/006925
Publication Date:
March 02, 2006
Filing Date:
June 28, 2005
Export Citation:
Assignee:
MERCK PATENT GMBH (DE)
GILLIES STEPHEN D (US)
WATKINS NIGEL JOHN (GB)
BAKER MATTHEW PAUL (GB)
LO KING-MING (US)
GILLIES STEPHEN D (US)
WATKINS NIGEL JOHN (GB)
BAKER MATTHEW PAUL (GB)
LO KING-MING (US)
International Classes:
A61K38/21; C07K14/565; A61K39/395; C07K19/00; C12N15/22; C12N15/62
Domestic Patent References:
| WO2001003737A1 | 2001-01-18 | |||
| WO2000023472A2 | 2000-04-27 | |||
| WO2000069913A1 | 2000-11-23 | |||
| WO2002074783A2 | 2002-09-26 |
Foreign References:
| US4737462A | 1988-04-12 |
Other References:
LO K-M ET AL: "High level expression and secretion of Fc-X fusion proteins in mammalian cells", PROTEIN ENGINEERING, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 11, no. 6, June 1998 (1998-06-01), pages 495 - 500, XP002125745, ISSN: 0269-2139
DAY C ET AL: "ENGINEERED DISULFIDE BOND GREATLY INCREASES SPECIFIC ACTIVITY OF RECOMBINANT MURINE INTERFERON- BETA", JOURNAL OF INTERFERON RESEARCH, MARY ANN LIEBERT, INC., NEW YORK, NY, US, vol. 12, no. 2, 1 April 1992 (1992-04-01), pages 139 - 143, XP000647532, ISSN: 0197-8357
STEWART A G ET AL: "CHEMICAL MUTAGENESIS OF HUMAN INTERFERON-BETA: CONSTRUCTION, EXPRESSION IN E. COLI, AND BIOLOGICAL ACTIVITY OF SODIUM BISULFITE-INDUCED MUTATIONS", DNA, MARY ANN LIEBERT, NEW YORK, NY, US, vol. 6, no. 2, April 1987 (1987-04-01), pages 119 - 128, XP009004161, ISSN: 0198-0238
RUNKEL LAURA ET AL: "Systematic mutational mapping of sites on human interferon-beta-1a that are important for receptor binding and functional activity", BIOCHEMISTRY, AMERICAN CHEMICAL SOCIETY. EASTON, PA, US, vol. 39, no. 10, 14 March 2000 (2000-03-14), pages 2538 - 2551, XP002160244, ISSN: 0006-2960
DAY C ET AL: "ENGINEERED DISULFIDE BOND GREATLY INCREASES SPECIFIC ACTIVITY OF RECOMBINANT MURINE INTERFERON- BETA", JOURNAL OF INTERFERON RESEARCH, MARY ANN LIEBERT, INC., NEW YORK, NY, US, vol. 12, no. 2, 1 April 1992 (1992-04-01), pages 139 - 143, XP000647532, ISSN: 0197-8357
STEWART A G ET AL: "CHEMICAL MUTAGENESIS OF HUMAN INTERFERON-BETA: CONSTRUCTION, EXPRESSION IN E. COLI, AND BIOLOGICAL ACTIVITY OF SODIUM BISULFITE-INDUCED MUTATIONS", DNA, MARY ANN LIEBERT, NEW YORK, NY, US, vol. 6, no. 2, April 1987 (1987-04-01), pages 119 - 128, XP009004161, ISSN: 0198-0238
RUNKEL LAURA ET AL: "Systematic mutational mapping of sites on human interferon-beta-1a that are important for receptor binding and functional activity", BIOCHEMISTRY, AMERICAN CHEMICAL SOCIETY. EASTON, PA, US, vol. 39, no. 10, 14 March 2000 (2000-03-14), pages 2538 - 2551, XP002160244, ISSN: 0006-2960
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