Title:
FLAVIN-BOUND GLUCOSE DEHYDROGENASE HAVING IMPROVED SUBSTRATE SPECIFICITY
Document Type and Number:
WIPO Patent Application WO/2013/065770
Kind Code:
A1
Abstract:
The present invention relates to a flavin-bound glucose dehydrogenase having high substrate specificity for d-glucose and reduced reactivity with d-xylose and/or maltose. More specifically, provided is a flavin-bound glucose dehydrogenase wherein at least one amino acid residue is substituted at a position corresponding to position 78, 79, 81, 121, 122, 123, 569, or 612 of flavin-bound glucose dehydrogenase derived from the genus Mucor. According to the present invention, even when large amounts of enzyme are loaded, as with a glucose sensor, for example, an impact from the presence of d-xylose and/or maltose is less likely, and it is possible to accurately determine the amount of d-glucose.
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Inventors:
ARAKI YASUKO (JP)
ICHIYANAGI ATSUSHI (JP)
ICHIKAWA KEIICHI (JP)
HIROKAWA KOZO (JP)
ICHIYANAGI ATSUSHI (JP)
ICHIKAWA KEIICHI (JP)
HIROKAWA KOZO (JP)
Application Number:
PCT/JP2012/078283
Publication Date:
May 10, 2013
Filing Date:
November 01, 2012
Export Citation:
Assignee:
KIKKOMAN CORP (JP)
International Classes:
C12N15/09; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/04; C12Q1/32; G01N33/66
Domestic Patent References:
| WO2010140431A1 | 2010-12-09 | |||
| WO2011068050A1 | 2011-06-09 | |||
| WO2009084616A1 | 2009-07-09 |
Foreign References:
| JP2010035448A | 2010-02-18 | |||
| JP2011115156A | 2011-06-16 | |||
| JP2008237210A | 2008-10-09 |
Other References:
YAMAOKA H ET AL.: "Site directed mutagenesis studies of FAD-dependent glucose dehydrogenase catalytic subunit of Burkholderia cepacia", BIOTECHNOL.LETT., vol. 30, no. 11, 30 November 2008 (2008-11-30), pages 1967 - 1972, XP019639462
Attorney, Agent or Firm:
TSUKUNI & ASSOCIATES et al. (JP)
Patent business corporation Tsukuni (JP)
Patent business corporation Tsukuni (JP)
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