Title:
FLUORESCENCE DETECTION METHOD, FLUORESCENCE DETECTION DEVICE AND PROGRAM
Document Type and Number:
WIPO Patent Application WO/2010/095386
Kind Code:
A1
Abstract:
A fluorescence detection method comprises a step for irradiating an object to be measured with laser light modulated at a predetermined frequency, a step for receiving fluorescence generated by the object to be measured and outputting a plurality of pulsed fluorescence signals, a step for setting the reference timing in each cycle corresponding to the frequency, a step for acquiring the generation time required until each of the pulsed fluorescence signals is outputted on the basis of the reference timing, a step for generating a cumulative fluorescence signal indicating the relationship between the generation frequency and the generation time of the pulsed fluorescence signal, and a step for, using a signal corresponding to the modulation of the laser light as a reference signal, finding the phase difference between the reference signal and the cumulative fluorescence signal, and a step for finding the fluorescence relaxation time of the fluorescence of the object to be measured using the phase difference.
Inventors:
HAYASHI HIRONORI (JP)
Application Number:
PCT/JP2010/000686
Publication Date:
August 26, 2010
Filing Date:
February 04, 2010
Export Citation:
Assignee:
MITSUI SHIPBUILDING ENG (JP)
HAYASHI HIRONORI (JP)
HAYASHI HIRONORI (JP)
International Classes:
G01N21/64
Foreign References:
| JP2007127415A | 2007-05-24 | |||
| JP2005019537A | 2005-01-20 | |||
| JP2000304697A | 2000-11-02 | |||
| JP2006226698A | 2006-08-31 |
Other References:
TETSUO IWATA ET AL.: "Photon-Counting Phase- Modulation Fluorometer", OPTICAL REVIEW, vol. 8, no. 5, 2001, pages 326 - 330, XP019353913
Attorney, Agent or Firm:
GLOBAL IP TOKYO (JP)
Global IP Tokyo patent business corporation (JP)
Global IP Tokyo patent business corporation (JP)
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