Title:
FLUORESCENT PROBE FOR DETECTING PEPTIDE-LINKED HYDROLASE
Document Type and Number:
WIPO Patent Application WO/2023/219136
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a fluorescent probe that can be used in an enzymatic activity detection method. The present invention provides a fluorescent probe for detecting a hydrolase, the probe including: a compound represented by general formula (II) and having at least one water-soluble substituent selected from the group consisting of -CO2H, -PO3H2 and -SO3H; or a salt thereof. The fluorescent probe according to the present invention can be used in an enzyme activity detection method using a microdevice.
Inventors:
URANO YASUTERU (JP)
KOMATSU TORU (JP)
KAGAMI YU (JP)
SAKAMOTO SHINGO (JP)
KASAI TAKAFUMI (JP)
WATANABE RIKIYA (JP)
KOMATSU TORU (JP)
KAGAMI YU (JP)
SAKAMOTO SHINGO (JP)
KASAI TAKAFUMI (JP)
WATANABE RIKIYA (JP)
Application Number:
PCT/JP2023/017768
Publication Date:
November 16, 2023
Filing Date:
May 11, 2023
Export Citation:
Assignee:
UNIV TOKYO (JP)
RIKEN (JP)
RIKEN (JP)
International Classes:
C12Q1/34; C07D493/10; C07F7/10; C09B11/28; C09B57/00; C09K11/06; C12Q1/37; G01N21/64; G01N21/78; G01N37/00
Domestic Patent References:
| WO2021173865A2 | 2021-09-02 | |||
| WO2013180181A1 | 2013-12-05 |
Foreign References:
| US20100233744A1 | 2010-09-16 | |||
| DE19843873A1 | 2000-03-30 | |||
| EP3960817A1 | 2022-03-02 |
Other References:
"Cellular Peptidases in Immune Functions and Diseases", 1 January 1997, SPRINGER US, ISBN: 978-1-4757-9613-1, article LOREY SUSAN, FAUST JÜRGEN, HERMANNS UTA, BÜHLING FRANK, ANSORGE SIEGFRIED, NEUBERT KLAUS: "New Fluorogenic Dipeptidyl Peptidase IV/CD26 Substrates and Inhibitors", pages: 157 - 160, XP009550266
BUTKEVICH ALEXEY N., WEBER MICHAEL, CERECEDA DELGADO ANGEL R., OSTERSEHLT LYNN M., D’ESTE ELISA, HELL STEFAN W.: "Photoactivatable Fluorescent Dyes with Hydrophilic Caging Groups and Their Use in Multicolor Nanoscopy", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, AMERICAN CHEMICAL SOCIETY, vol. 143, no. 44, 10 November 2021 (2021-11-10), pages 18388 - 18393, XP055910632, ISSN: 0002-7863, DOI: 10.1021/jacs.1c09999
BUTKEVICH ALEXEY N., WEBER MICHAEL, CERECEDA DELGADO ANGEL R., OSTERSEHLT LYNN M., D’ESTE ELISA, HELL STEFAN W.: "Photoactivatable Fluorescent Dyes with Hydrophilic Caging Groups and Their Use in Multicolor Nanoscopy", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, AMERICAN CHEMICAL SOCIETY, vol. 143, no. 44, 10 November 2021 (2021-11-10), pages 18388 - 18393, XP055910632, ISSN: 0002-7863, DOI: 10.1021/jacs.1c09999
Attorney, Agent or Firm:
FIELDS IP ATTORNEYS PPC (JP)
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