Title:
FLUORESCENT PROTEIN
Document Type and Number:
WIPO Patent Application WO/2015/037674
Kind Code:
A1
Abstract:
Provided is a fluorescent protein having a rapid light switching speed and high light stability, the fluorescent protein changing from nonfluorescent to fluorescent upon photoirradiation for fluorescence excitation and changing from fluorescent to nonfluorescent upon photoirradiation of a specific wavelength that does not produce fluorescence excitation. Relating to a fluorescent protein shown by the following (a) or (b). (a) A protein having the amino acid sequence listed in SEQ ID NO: 1. (b) A protein having an amino acid sequence in which from one to several amino acids have been deleted, substituted and/or added in the amino acid sequence listed in SEQ ID NO: 1, the protein being capable of functioning as a fluorescent protein that changes from nonfluorescent to fluorescent upon photoirradiation for fluorescence excitation and changes from fluorescent to nonfluorescent upon photoirradiation of a specific wavelength that does not produce fluorescence excitation.
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Inventors:
NAGAI TAKEHARU (JP)
TIWARI DHERMENDRA KUMAR (JP)
ARAI YOSHIYUKI (JP)
TIWARI DHERMENDRA KUMAR (JP)
ARAI YOSHIYUKI (JP)
Application Number:
PCT/JP2014/074121
Publication Date:
March 19, 2015
Filing Date:
September 11, 2014
Export Citation:
Assignee:
UNIV OSAKA (JP)
International Classes:
C12N15/09; C07K14/435; C07K19/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10
Domestic Patent References:
| WO2005113772A1 | 2005-12-01 | |||
| WO2005113772A1 | 2005-12-01 |
Other References:
ANDRESEN, M. ET AL.: "Photoswitchable fluorescent proteins enable monochromatic multilabel imaging and dual color fluorescence nanoscopy", NAT. BIOTECHNOL., vol. 26, no. 9, 2008, pages 1035 - 1040, XP055015979
BRAKEMANN, T. ET AL.: "Molecular Basis of the Light-driven Switching of the Photochromic Fluorescent Protein Padron", J. BIOL. CHEM., vol. 285, no. 19, 2010, pages 14603 - 14609, XP055327180
TANJA BRAKEMANN ET AL., J. BIOL. CHEM., vol. 285, 2010, pages 14603 - 14609
GAYDA, S.; NIENHAUS, K.; NIENHAUS, G.U., BIOPHYSICAL JOURNAL, vol. 103, 2012, pages 2521 - 31
See also references of EP 3045534A4
BRAKEMANN, T. ET AL.: "Molecular Basis of the Light-driven Switching of the Photochromic Fluorescent Protein Padron", J. BIOL. CHEM., vol. 285, no. 19, 2010, pages 14603 - 14609, XP055327180
TANJA BRAKEMANN ET AL., J. BIOL. CHEM., vol. 285, 2010, pages 14603 - 14609
GAYDA, S.; NIENHAUS, K.; NIENHAUS, G.U., BIOPHYSICAL JOURNAL, vol. 103, 2012, pages 2521 - 31
See also references of EP 3045534A4
Attorney, Agent or Firm:
IKEUCHI SATO & PARTNER PATENT ATTORNEYS (JP)
Patent business corporation Ikeuchi and Sato and partners (JP)
Patent business corporation Ikeuchi and Sato and partners (JP)
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