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Title:
FLUOROIMMUNOASSSAY METHOD
Document Type and Number:
WIPO Patent Application WO/2011/061944
Kind Code:
A1
Abstract:
Disclosed is an immunoassay method which does not require a solid phase immobilization step and a washing step, enables the rapid, simple and quantitative measurement of a substance of interest in a liquid phase, and enables the visualization of an antigen. The concentration of an antigen of interest in a substance to be tested can be measured by carrying out the followings steps (a) to (c) sequentially: (a) bringing an antibody light-chain variable region polypeptide and an antibody heavy-chain variable region polypeptide labeled with a fluorescent dye into contact with the antigen in the substance to be tested in a liquid phase, or bringing an antibody heavy-chain variable region polypeptide and an antibody light-chain variable region polypeptide labeled with a fluorescent dye into contact with the antigen in the substance to be tested in a liquid phase; (b) measuring the fluorescence intensity of the fluorescent dye; and (c) calculating the amount of the antigen contained in the substance to be tested on the basis of the positive correlation between the concentration of the antigen in the liquid phase and the fluorescence intensity of the fluorescent dye.

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Inventors:
UEDA HIROSHI (JP)
ABE RYOJI (JP)
IHARA MASAKI (JP)
TAKAGI HIROAKI (JP)
Application Number:
PCT/JP2010/006809
Publication Date:
May 26, 2011
Filing Date:
November 19, 2010
Export Citation:
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Assignee:
PROTEINEXPRESS CO LTD (JP)
UEDA HIROSHI (JP)
ABE RYOJI (JP)
IHARA MASAKI (JP)
TAKAGI HIROAKI (JP)
International Classes:
G01N33/533; G01N21/78; G01N33/531; C07K16/18
Domestic Patent References:
WO2008051762A22008-05-02
Foreign References:
JPH10319017A1998-12-04
JP2006506634A2006-02-23
JPH1078436A1998-03-24
JP2007526750A2007-09-20
JP2008187936A2008-08-21
JPH1078436A1998-03-24
JP3784111B22006-06-07
JPH10282098A1998-10-23
JP2000139468A2000-05-23
US5643722A1997-07-01
Other References:
SHEAN-LEE LIM ET AL.: "Noncompetitive Detection of Low Molecular Weight Peptides by Open Sandwich Immunoassay", ANALYTICAL CHEMISTRY, vol. 79, no. 16, 2007, pages 6193 - 6200, XP008156568
TOMOICHI YOKOZEKI ET AL.: "A Homogeneous Noncompetitive Immunoassay for the Detection of Small Haptens", ANALYTICAL CHEMISTRY, vol. 74, no. 11, 2002, pages 2500 - 2504, XP008156580
HIROSHI UEDA, JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN, vol. 27, 2007, pages 71 - 80
LIM SL ET AL., ANAL CHEM., vol. 79, no. 16, 2007, pages 6193 - 200
IIJIMA I.; HOHSAKA T., CHEMBIOCHEM., vol. 10, no. 6, 2009, pages 999 - 1006
KAJIHARA D ET AL., NAT METHODS, vol. 3, no. 11, 2006, pages 923
ELLMAN J ET AL., METHODS ENZYMOL., vol. 202, 1991, pages 301 - 36
OLEJNIK ET AL., METHODS, vol. 36, 2005, pages 252 - 260
KABAT, E. ET AL.: "Sequences of proteins of immunological interest", 1991, NATIONAL INSTITUTE OF HEALTH
DR. ANDREW C.R. MARTIN'S GROUP, MARTIN, A.C.R. ACCESSING THE KABAT ANTIBODY SEQUENCE DATABASE BY COMPUTER PROTEINS: STRUCTURE, FUNCTION AND GENETICS, vol. 25, 1996, pages 130 - 133, Retrieved from the Internet
See also references of EP 2515110A4
Attorney, Agent or Firm:
HIROTA, Masanori (JP)
Masaki Hirota (JP)
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