FORENSIC DNA PROFILING METHOD COMPRISING IMMUNOLOGICAL SEPARATION STEP

Title:

FORENSIC DNA PROFILING METHOD COMPRISING IMMUNOLOGICAL SEPARATION STEP

Document Type and Number:

WIPO Patent Application WO/2000/077251

Kind Code:

A2

Abstract:

A DNA profiling method which comprises (i) contacting a sample believed to comprise spermatozoa and/or epithelial cells with antibodies specific for antigens presented on the spermatozoa and/or epithelial cells which antigens are not readily degraded, so as to form antibody-spermatozoa and/or antibody-epithelial cell complexes, (ii) separating the complex(es) from other material in the sample, (iii) isolating DNA from the complex(es) and (iv) determining the specific characteristics of the isolated DNA.

Inventors:

GREENHALGH MATTHEW JAMES (GB)

Application Number:

PCT/GB2000/002203

Publication Date:

December 21, 2000

Filing Date:

June 07, 2000

Export Citation:

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Assignee:

ASTRAZENECA UK LTD (GB)
GREENHALGH MATTHEW JAMES (GB)

International Classes:

G01N33/53; G01N33/68; (IPC1-7): C12Q1/68

Domestic Patent References:

WO1998028622A1	1998-07-02
WO1999042129A1	1999-08-26

Foreign References:

EP0530009A2	1993-03-03
EP0238329A2	1987-09-23
EP0186271A1	1986-07-02
US5830472A	1998-11-03
US5605803A	1997-02-25
US5436157A	1995-07-25
US5047508A	1991-09-10
US4741998A	1988-05-03

Attorney, Agent or Firm:

Phillips, Neil Godfrey Alasdair (Alderley Park P.O. Box 272 Macclesfield, Cheshire SK10 4GR, GB)

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Claims:

1.

A DNA profiling method which comprises (i) contacting a sample believed to comprise spermatozoa and/or epithelial cells with antibodies specific for antigens presented on the spermatozoa and/or epithelial cells which antigens are not readily degraded, so as to form antibodyspermatozoa and/or antibodyepithelial cell complexes, (ii) separating the complex (es) from other material in the sample, (iii) isolating DNA from the complex (es) and (iv) determining the specific characteristics of the isolated DNA.

2.	A method as claimed in claim 1 wherein the antibodies are biotinylated for separation in step (ii).

3.	A method as claimed in claim 1 wherein the antibodies are conjugated to magnetic beads for separation in step (ii).

4.	A method as claimed in claim 1 wherein secondary antibodies are used for separation in step (ii).

5.

A DNA profiling method which comprises (i) contacting a sample believed to comprise both spermatozoa and epithelial cells with antibodies specific for antigens presented on the spermatozoa and epithelial cells respectively which antigens are not readily degraded, so as to form antibodyspermatozoa and antibodyepithelial cell complexes respectively, (ii) separating the complexes from other material in the sample, (iii) isolating DNA from one or both complexes and (iv) determining the specific characteristics of the isolated DNA.

6.	A method as claimed in any one of the previous claims wherein the antibodies for at least one antigen are monoclonal.

7.	A method as claimed in any one of the previous claims wherein the antibodies have been raised to the FA2 antigen.

8.	A method as claimed in any one of claims 16 wherein the antibodies have been raised to the Sperm Agglutination Antigen1 (SAGA1).

Description:

INTERNATIONAL SEARCH j-----------------. Intern I Applfcation No PCT/GB 00/02203 C. (Continuation) DOCUMENTS CONSIDERED TO BE RELEVANT Category Citation of document, with indication, where appropriate, of the relevant passages Relevant to claim No. Y WO 98 28622 A (FODSTAD OEYSTEIN; HOEIFOEDT 1-8 HANNE KLEPPE (NO)) 2 July 1998 (1998-07-02) claims1,5-9,11 page 5, line 5-line 13 page 6, line 7-line 24 page 8, line 19 Y US 5 830 472 A (HERR JOHN C ET AL) 1-8 3 November 1998 (1998-11-03) the whole document Y US 5 605 803 A (HERR JOHN C ET AL) 1-8 25 February 1997 (1997-02-25) claims column 5, line 25-column 6, line 25 Y US 5 436 157 A (HERR JOHN C ET AL) 1-8 25 July 1995 (1995-07-25) column 8, line 23-line 48 column 13, line 26-line 47 Y US 5 047 508 A (HERR JOHN C ET AL) 1-8 10 September 1991 (1991-09-10) abstract column 2, line 13-line 50 column 11, line 41-line 54 Y US 4 741 998 A (HERR JOHN C ET AL) 1-8 3 May 1988 (1988-05-03) the whole document P, Y WO 99 42129 A (UNIV VIRGINIA) 1-8 26 August 1999 (1999-08-26) claims 10-12 page 1, paragraph 2 page 6, last paragraph-page 7, paragraph 2 page 16, last paragraph-page 17, paragraph 1 INTERNATIONAL SEARCH REPORT Intern ; I Appiication No . rmation on patent family members PCT/GB 00/02203 Patent document | Publication | Patent cited in search report date member (s) date EP 0530009 A 03-03-1993 AT 175722 T 15-01-1999 AT 190096 T 15-03-2000 AU 660690 B 06-07-1995 AU 2129592 A 04-03-1993 CA 2077264 A 28-02-1993 DE 69228158 D 25-02-1999 DE 69228158 T 02-09-1999 DE 69230736 D 06-04-2000 DE 69230736 T 21-06-2000 EP 0731177 A 11-09-1996 ES 2143680 T 16-05-2000 GB 2259138 A, B 03-03-1993 JP 6205700 A 26-07-1994 NZ 244089 A 27-01-1995 US 5811235 A 22-09-1998 US 5853989 A 29-12-1998 EP 0238329 A 23-09-1987 AT 105024 T 15-05-1994 AU 604686 B 03-01-1991 AU 7070187 A 07-01-1988 AU 7738887 A 07-01-1988 CN 87102927 A 27-04-1988 DE 3789679 D 01-06-1994 DE 3789679 T 25-08-1994 DK 140287 A 20-09-1987 FI 871189 A 20-09-1987 GB 2188323 A, B 30-09-1987 HU 44800 A 28-04-1988 IE 59859 B 20-04-1994 IN 168721 A 25-05-1991 JP 1907803 C 24-02-1995 JP 6030636 B 27-04-1994 JP 62282600 A 08-12-1987 JP 5276948 A 26-10-1993 NO 871122 A 21-09-1987 NZ 219685 A 26-02-1990 PT 84502 A, B 01-04-1987 SU 1788970 A 15-01-1993 US 5175082 A 29-12-1992 YU 47887 A 28-02-1991 ZA 8701993 A 30-12-1987 EP 0186271 A 02-07-1986 AT 70564 T 15-01-1992 AU 581582 B 23-02-1989 AU 4962685 A 03-06-1986 BR 8507049 A 10-03-1987 CN 85109013 A, B 30-07-1986 CY 1427 A 02-09-1988 DE 3584957 D 30-01-1992 DE 186271 T 15-12-1988 DK 331886 A 11-07-1986 EP 0342717 A 23-11-1989 ES 548749 D 01-11-1987 ES 8800349 A 01-01-1988 FI 862915 A 11-07-1986 WO 8602948 A 22-05-1986 GB 2166445 A, B 08-05-1986 GR 852708 A 10-03-1986 INTERNATIONAL SEARCH REPORT , rmation on patent family members PCT/GB ûO/02203 PCT/GB 00/02203 Patent document Publication Patent family Publication cited in search report date member (s) date EP 0186271 A HK 13989 A 24-02-1989 HU 46078 A 28-09-1988 HU 203795 B 30-09-1991 IE 62864 B 08-03-1995 IN 162779 A 09-07-1988 JP 2750228 B 13-05-1998 JP 6253844 A 13-09-1994 JP 4063680 B 12-10-1992 JP 62501467 T 18-06-1987 KE 3800 A 29-04-1988 NZ 213762 A 29-03-1989 PL 256209 A 23-09-1986 PT 81468 A, B 01-12-1985 SG 12688 G 08-07-1988 US 5413908 A 09-05-1995 YU 176985 A 30-06-1991 ZA 8508045 A 24-06-1987 WO 9828622 A 02-07-1998 NO 965531 A 22-06-1998 AU 7875298 A 17-07-1998 EP 0951645 A 27-10-1999 US 5830472 A 03-11-1998 AU 722559 B 03-08-2000 AU 3407197 A 21-01-1998 BR 9710089 A 06-06-2000 CA 2258717 A 08-01-1998 CN 1227497 A 01-09-1999 CZ 9804284 A 16-06-1999 EP 0964696 A 22-12-1999 HU 9904620 A 28-05-2000 NO 986050 A 08-02-1999 PL 330842 A 07-06-1999 SK 179598 A 10-04-2000 WO 9800164 A 08-01-1998 US 5605803 A 25-02-1997 US 5436157 A 25-07-1995 AU 693493 B 02-07-1998 AU 1127295 A 16-11-1995 CA 2188397 A 02-11-1995 EP 0757696 A 12-02-1997 JP 9512338 T 09-12-1997 WO 9529188 A 02-11-1995 US 5602005 A 11-02-1997 US 5753231 A 19-05-1998 AT 176156 T 15-02-1999 AU 649609 B 02-06-1994 AU 5186290 A 26-09-1990 DE 69032922 D 11-03-1999 DE 69032922 T 30-09-1999 DK 461177 T 13-09-1999 EP 0461177 A 18-12-1991 ES 2130121 T 01-07-1999 JP 4505008 T 03-09-1992 NO 913428 A 18-10-1991 WO 9009802 A 07-09-1990 US 5436157 A 25-07-1995 AT 176156 T 15-02-1999 AU 649609 B 02-06-1994 INTERNATIONAL SEARCH REPORT Intern 1 Appilcation No , rmatlon on patent famify members PCT/GB 00/02203 Patent document Publication Patent Publication cited in search report date member (s) date US 5436157 A AU 5186290 A 26-09-1990 DE 69032922 D 11-03-1999 DE 69032922 T 30-09-1999 DK 461177 T 13-09-1999 EP 0461177 A 18-12-1991 ES 2130121 T 01-07-1999 JP 4505008 T 03-09-1992 NO 913428 A 18-10-1991 WO 9009802 A 07-09-1990 US 5605803 A 25-02-1997 US 5602005 A 11-02-1997 US 5753231 A 19-05-1998 US 5047508 A 10-09-1991 US 4741998 A 03-05-1988 EP 0222774 A 27-05-1987 JP 63500204 T 21-01-1988 WO 8607381 A 18-12-1986 US 4741998 A 03-05-1988 EP 0222774 A 27-05-1987 JP 63500204 T 21-01-1988 US 5047508 A 10-09-1991 WO 8607381 A 18-12-1986 WO 9942129 A 26-08-1999 AU 2759899 A 06-09-1999

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