Title:
FUSION PEPTIDES ISOLATABLE BY PHASE TRANSITION
Document Type and Number:
WIPO Patent Application WO2002074928
Kind Code:
A3
Abstract:
Genetically-encodable, environmentally-responsive fusion proteins comprising ELP peptides. Such fusion proteins exhibit unique physico-chemical and functional properties that can be modulated as a function of solution environment. The invention also provides methods for purifying the FPs, which take advantage of these unique properties, including high-throughput purification methods.
Inventors:
CHILKOTI ASHUTOSH (US)
Application Number:
PCT/US2002/008523
Publication Date:
March 06, 2003
Filing Date:
March 20, 2002
Export Citation:
Assignee:
CHILKOTI ASHUTOSH (US)
International Classes:
C07K14/36; C07K14/78; C12N9/02; C12N15/62; C12P21/04; (IPC1-7): C12P21/04; C07H21/04; C07K14/00; C07K14/435; C12N9/00
Foreign References:
| US20020034050A1 | 2002-03-21 |
Other References:
MEYER ET AL.: "Purification of recombinant proteins by fusion with thermally-responsive polypeptides", NATURE BIOTECHNOLOGY, vol. 17, November 1999 (1999-11-01), pages 1112 - 1115, XP002955912
MEYER ET AL.: "Protein purification by fusion with environmentally responsive elastin-like polypetide: effect of polypeptide length on the purification of thioredoxin", BIOTECHNOL. PROG., vol. 17, no. 4, 2001, pages 720 - 728, XP002955911
CHILKOTI A.: "Biomedical applications of genetically encoded biopolymers", 222ND ACS NATIONAL MEETING (MACR-019), 26 August 2001 (2001-08-26) - 30 August 2001 (2001-08-30), CHIGAGO, IL, USA, XP002954796
MEYER ET AL.: "Polypeptide fusion tag for thermal purification of recombinant proteins", 217TH ACS NATIONAL MEETING (BIOT-078), 21 March 1999 (1999-03-21) - 25 March 1999 (1999-03-25), ANAHEIM, CALIF., XP002954797
MCPERSON ET AL.: "Product purfication by reversible phase transition following escherichia coli expression of genes encoding up to 251 repeats of the elastomeric pentapeptide GVGVP", PROTEIN EXPRESSION AND PURIFICATION, vol. 7, 1996, pages 51 - 57, XP002955913
MEYER ET AL.: "Protein purification by fusion with environmentally responsive elastin-like polypetide: effect of polypeptide length on the purification of thioredoxin", BIOTECHNOL. PROG., vol. 17, no. 4, 2001, pages 720 - 728, XP002955911
CHILKOTI A.: "Biomedical applications of genetically encoded biopolymers", 222ND ACS NATIONAL MEETING (MACR-019), 26 August 2001 (2001-08-26) - 30 August 2001 (2001-08-30), CHIGAGO, IL, USA, XP002954796
MEYER ET AL.: "Polypeptide fusion tag for thermal purification of recombinant proteins", 217TH ACS NATIONAL MEETING (BIOT-078), 21 March 1999 (1999-03-21) - 25 March 1999 (1999-03-25), ANAHEIM, CALIF., XP002954797
MCPERSON ET AL.: "Product purfication by reversible phase transition following escherichia coli expression of genes encoding up to 251 repeats of the elastomeric pentapeptide GVGVP", PROTEIN EXPRESSION AND PURIFICATION, vol. 7, 1996, pages 51 - 57, XP002955913
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