Title:
FUSION PROTEIN BETWEEN ANTIGEN-BINDING MOLECULE AND STREPTAVIDIN VARIANT
Document Type and Number:
WIPO Patent Application WO/2022/203000
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing a fusion protein that is for use in treatment or diagnosis of cancer and that is formed between a streptavidin variant and a molecule that recognizes a cancer cell or the like. The present invention provides a fusion protein in which an antigen-binding molecule having a molecular weight of 20,000 or less is bound to, via a linker sequence, the N-terminal side and/or the C-terminal side of an amino acid sequence described in SEQ ID NO: 1 (wherein, the amino acid sequence of Pro-Ser-Ala-Ala-Ser His-His-His-His-His-His at the C-terminal may be partially or totally deleted).
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Inventors:
TANAKA TOSHIYA (JP)
KODAMA TATSUHIKO (JP)
KANAI MOTOMU (JP)
YAMATSUGU KENZO (JP)
TATSUMI TOSHIFUMI (JP)
TAKAHASHI KAZUKI (JP)
SUGIYAMA AKIRA (JP)
TSUKAGOSHI MASANOBU (JP)
KODAMA TATSUHIKO (JP)
KANAI MOTOMU (JP)
YAMATSUGU KENZO (JP)
TATSUMI TOSHIFUMI (JP)
TAKAHASHI KAZUKI (JP)
SUGIYAMA AKIRA (JP)
TSUKAGOSHI MASANOBU (JP)
Application Number:
PCT/JP2022/013994
Publication Date:
September 29, 2022
Filing Date:
March 24, 2022
Export Citation:
Assignee:
UNIV TOKYO (JP)
SAVID THERAPEUTICS INC (JP)
SAVID THERAPEUTICS INC (JP)
International Classes:
C07K19/00; A61K31/695; A61K38/16; A61K39/395; A61K41/00; A61K45/00; A61K47/54; A61K47/66; A61K47/68; A61K49/00; A61P35/00; C07K4/00; C07K14/00; C07K14/36; C07K16/28; C07K16/30; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N15/11; C12N15/13; C12N15/31; C12N15/62; C12P21/02; C12P21/08; G01N33/53; G01N33/574
Domestic Patent References:
| WO2020138427A1 | 2020-07-02 | |||
| WO2015125820A1 | 2015-08-27 |
Foreign References:
| JP6127045B2 | 2017-05-10 | |||
| JP2017524659A | 2017-08-31 |
Other References:
FREDRIK Y FREJD, KYU-TAE KIM: "Affibody molecules as engineered protein drugs", EXPERIMENTAL & MOLECULAR MEDICINE, vol. 49, no. 3, 1 March 2017 (2017-03-01), pages e306 - e306, XP055721379, DOI: 10.1038/emm.2017.35
LI SHUANG, HAO ZHI-MING: "An Engineered Affinity Protein-affibody", SHENGWU HUAXUE YU SHENGWU WULI JINZHAN - BIOCHEMISTRY AND BIOPHYSICS, KEXUE CHUBANSHE, BEIJING, CN, vol. 39, no. 2, 31 March 2012 (2012-03-31), CN , pages 137 - 141, XP055969212, ISSN: 1000-3282, DOI: 10.3724/SP.J.1206.2011.00269
LÖFBLOM J., FELDWISCH J., TOLMACHEV V., CARLSSON J., STÅHL S., FREJD F.Y.: "Affibody molecules: Engineered proteins for therapeutic, diagnostic and biotechnological applications", FEBS LETTERS, ELSEVIER, AMSTERDAM., NL, vol. 584, no. 12, 11 April 2010 (2010-04-11), NL , pages 2670 - 2680, XP002807295, ISSN: 0014-5793, DOI: 10.1016/J.FEBSLET.2010.04.014
SEMERADTOVA ALENA, STOFIK MARCEL, VANKOVA LUCIE, MALY PETR, STANEK ONDREJ, MALY JAN: "Optical microchips based on high-affinity recombinant protein binders—Human serum albumin detection in urine", SENSORS AND ACTUATORS B: CHEMICAL, ELSEVIER BV, NL, vol. 272, 1 November 2018 (2018-11-01), NL , pages 441 - 447, XP055969214, ISSN: 0925-4005, DOI: 10.1016/j.snb.2018.05.180
YAMATSUGU KENZO; KATOH HIROTO; YAMASHITA TAKEFUMI; TAKAHASHI KAZUKI; AKI SHO; TATSUMI TOSHIFUMI; KANEKO YUDAI; KAWAMURA TAKESHI; M: "Antibody mimetic drug conjugate manufactured by high-yield Escherichia coli expression and non-covalent binding system", PROTEIN EXPRESSION AND PURIFICATION, ACADEMIC PRESS, SAN DIEGO, CA., vol. 192, 30 December 2021 (2021-12-30), SAN DIEGO, CA. , XP086926729, ISSN: 1046-5928, DOI: 10.1016/j.pep.2021.106043
MULLIGAN ET AL., NATURE, vol. 277, 1979, pages 108
MIZUSHIMA ET AL., NUCLEIC ACIDS RES., vol. 18, 1990, pages 5322
LOFBLOM, J. FEBS LETT., vol. 584, no. 12, 2010, pages 2670 - 80
YAMATSUGU KKATOH HYAMASHITA T ET AL.: "Antibody mimetic drug conjugate manufactured by high-yield Escherichia coli expression and non-covalent binding system", PROTEIN EXPR PURIF., vol. 192, 2022, pages 106043
TAKAHASHI KSUGIYAMA AOHKUBO K ET AL.: "Axially substituted silicon phthalocyanine payloads for antibody-drug conjugates", SYNLETT., vol. 32, 2021, pages 1098 - 1103
SUGIYAMA AKAWAMURA TTANAKA T ET AL.: "Cupid and Psyche system for the diagnosis and treatment of advanced cancer", PROC JPN ACAD SER B PHYS BIOL SCI., vol. 95, 2019, pages 602 - 611, XP093028615, DOI: 10.2183/pjab.95.041
KUREBAYASHI JOTSUKI TTANG CK ET AL.: "Isolation and characterization of a new human breast cancer cell line, KPL-4, expressing the Erb B family receptors and interleukin-6", BR J CANCER., vol. 79, 1999, pages 707 - 717
YAMATSUGU KKATOH HYAMASHITA T ET AL.: "Antibody mimetic drug conjugate manufactured by high-yield", PROTEIN EXPR PURIF., vol. 192, 2022, pages 106043
LI SHUANG, HAO ZHI-MING: "An Engineered Affinity Protein-affibody", SHENGWU HUAXUE YU SHENGWU WULI JINZHAN - BIOCHEMISTRY AND BIOPHYSICS, KEXUE CHUBANSHE, BEIJING, CN, vol. 39, no. 2, 31 March 2012 (2012-03-31), CN , pages 137 - 141, XP055969212, ISSN: 1000-3282, DOI: 10.3724/SP.J.1206.2011.00269
LÖFBLOM J., FELDWISCH J., TOLMACHEV V., CARLSSON J., STÅHL S., FREJD F.Y.: "Affibody molecules: Engineered proteins for therapeutic, diagnostic and biotechnological applications", FEBS LETTERS, ELSEVIER, AMSTERDAM., NL, vol. 584, no. 12, 11 April 2010 (2010-04-11), NL , pages 2670 - 2680, XP002807295, ISSN: 0014-5793, DOI: 10.1016/J.FEBSLET.2010.04.014
SEMERADTOVA ALENA, STOFIK MARCEL, VANKOVA LUCIE, MALY PETR, STANEK ONDREJ, MALY JAN: "Optical microchips based on high-affinity recombinant protein binders—Human serum albumin detection in urine", SENSORS AND ACTUATORS B: CHEMICAL, ELSEVIER BV, NL, vol. 272, 1 November 2018 (2018-11-01), NL , pages 441 - 447, XP055969214, ISSN: 0925-4005, DOI: 10.1016/j.snb.2018.05.180
YAMATSUGU KENZO; KATOH HIROTO; YAMASHITA TAKEFUMI; TAKAHASHI KAZUKI; AKI SHO; TATSUMI TOSHIFUMI; KANEKO YUDAI; KAWAMURA TAKESHI; M: "Antibody mimetic drug conjugate manufactured by high-yield Escherichia coli expression and non-covalent binding system", PROTEIN EXPRESSION AND PURIFICATION, ACADEMIC PRESS, SAN DIEGO, CA., vol. 192, 30 December 2021 (2021-12-30), SAN DIEGO, CA. , XP086926729, ISSN: 1046-5928, DOI: 10.1016/j.pep.2021.106043
MULLIGAN ET AL., NATURE, vol. 277, 1979, pages 108
MIZUSHIMA ET AL., NUCLEIC ACIDS RES., vol. 18, 1990, pages 5322
LOFBLOM, J. FEBS LETT., vol. 584, no. 12, 2010, pages 2670 - 80
YAMATSUGU KKATOH HYAMASHITA T ET AL.: "Antibody mimetic drug conjugate manufactured by high-yield Escherichia coli expression and non-covalent binding system", PROTEIN EXPR PURIF., vol. 192, 2022, pages 106043
TAKAHASHI KSUGIYAMA AOHKUBO K ET AL.: "Axially substituted silicon phthalocyanine payloads for antibody-drug conjugates", SYNLETT., vol. 32, 2021, pages 1098 - 1103
SUGIYAMA AKAWAMURA TTANAKA T ET AL.: "Cupid and Psyche system for the diagnosis and treatment of advanced cancer", PROC JPN ACAD SER B PHYS BIOL SCI., vol. 95, 2019, pages 602 - 611, XP093028615, DOI: 10.2183/pjab.95.041
KUREBAYASHI JOTSUKI TTANG CK ET AL.: "Isolation and characterization of a new human breast cancer cell line, KPL-4, expressing the Erb B family receptors and interleukin-6", BR J CANCER., vol. 79, 1999, pages 707 - 717
YAMATSUGU KKATOH HYAMASHITA T ET AL.: "Antibody mimetic drug conjugate manufactured by high-yield", PROTEIN EXPR PURIF., vol. 192, 2022, pages 106043
Attorney, Agent or Firm:
SIKS & CO. (JP)
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