Title:
GENETICALLY ENCODED FORMALDEHYDE REACTIVE UNNATURAL AMINO ACID, PREPARATION METHOD AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2021/248770
Kind Code:
A1
Abstract:
A genetically encoded formaldehyde reactive unnatural amino acid. Specifically, the present invention relates to a genetically encoded formaldehyde reactive unnatural amino acid PrAK, a preparation method and an application thereof in detection and imaging of formaldehyde. The genetically encoded formaldehyde reactive unnatural amino acid PrAK can specifically introduce sites into biomacromolecules such as a green fluorescent protein (EGFP) and firefly luciferase (fLuc) in the protein translation process, construct a reactive biomacromolecule formaldehyde fluorescent probe and a bioluminescent probe, and can effectively detect or image formaldehyde in a biospecimen.
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Inventors:
PENG TAO (CN)
ZHANG YUQING (CN)
DU YIMENG (CN)
ZHANG YUQING (CN)
DU YIMENG (CN)
Application Number:
PCT/CN2020/123519
Publication Date:
December 16, 2021
Filing Date:
October 26, 2020
Export Citation:
Assignee:
UNIV PEKING SHENZHEN GRADUATE SCHOOL (CN)
International Classes:
C07C271/22; C07C213/02; C07C217/46; C07C269/04; C07C269/06; C07C271/16; C09K11/06; C12N9/00; C12N9/02; C12N15/52; C12P21/00
Domestic Patent References:
| WO2006001832A2 | 2006-01-05 |
Foreign References:
| CN110062808A | 2019-07-26 | |||
| CN101952428A | 2011-01-19 | |||
| CN107022568A | 2017-08-08 | |||
| CN104099360A | 2014-10-15 | |||
| CN104450746A | 2015-03-25 | |||
| CN111747869A | 2020-10-09 |
Other References:
ZHANG YUQING, DU YIMENG, LI MANJIA, ZHANG DONG, XIANG ZHENG, PENG TAO: "Activity‐Based Genetically Encoded Fluorescent and Luminescent Probes for Detecting Formaldehyde in Living Cells", ANGEWANDTE CHEMIE, vol. 59, no. 38, 14 September 2020 (2020-09-14), pages 16352 - 16356, XP055878600, ISSN: 1433-7851, DOI: 10.1002/anie.202001425
Attorney, Agent or Firm:
BEIJING POWER PATENT AGENCY FIRM (CN)
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