Title:
HEMATOLOGICAL ASSAY AND REAGENT
Document Type and Number:
WIPO Patent Application WO2001044819
Kind Code:
A3
Abstract:
A hematological assay is described in which the blood coagulation potential of a body fluid is assessed by reacting a sample of the body fluid with an amount of an activator reagent comprising: (a) a predetermined amount of factor Xa or a hematologically equivalent mutant thereof, and (b) a predetermined amount of factor Va, a hematologically equivalent mutant thereof or an enzyme activating endogenous factor V, (c) (optionally) phospholipids. The reagent may be dry (e.g. lyophilised) or in an aqueous solution preferably buffered to a pH from 6 to 10 (preferably 7 to 8), if desired incubating, if necessary inducing coagulation by the addition of one or more coagulation accelerants such as calcium chloride, and establishing a value indicative of the coagulation potential, e.g. by measuring the time to clotting on an optical coagulometer or through use of a chromogenic substrate. It is preferred to use at (b) factor V activator from purified Russell's Viper venom (RVV-V). An activator reagent is also described containing the components mentioned above preferably in one or more buffer solutions or in dried, e.g. lyophilised form.
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Inventors:
GEMPELER PATRIZIA MARIA (CH)
CALATZIS ANDREAS (DE)
CALATZIS ANDREAS (DE)
Application Number:
PCT/EP2000/012753
Publication Date:
December 06, 2001
Filing Date:
December 14, 2000
Export Citation:
Assignee:
PENTAPHARM AG (CH)
GEMPELER PATRIZIA MARIA (CH)
CALATZIS ANDREAS (DE)
GEMPELER PATRIZIA MARIA (CH)
CALATZIS ANDREAS (DE)
International Classes:
C12Q1/56; G01N33/483; G01N33/48; G01N33/49; G01N33/86; (IPC1-7): G01N33/86; C12Q1/56
Foreign References:
| US5059525A | 1991-10-22 | |||
| US4946775A | 1990-08-07 |
Other References:
HUISSE M-G ET AL: "PROTHROMBIN CLAMART PROTHROMBIN VARIANT WITH DEFECTIVE ARGININE 320-IIE CLEAVAGE BY FACTOR-XA", THROMBOSIS RESEARCH, vol. 44, no. 1, 1986, pages 11 - 22, XP001012065, ISSN: 0049-3848
VAN RIJN J L M L ET AL: "KINETIC STUDIES OF PROTHROMBIN ACTIVATION EFFECT OF FACTOR V-A AND PHOSPHOLIPIDS ON THE FORMATION OF THE ENZYME-SUBSTRATE COMPLEX", BIOCHEMISTRY, vol. 23, no. 20, 1984, pages 4557 - 4564, XP002171301, ISSN: 0006-2960
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; May 2000 (2000-05-01), CALATZIS AN ET AL: "Prothrombinase-induced clotting time (PiCT): A new assay for the monitoring of heparins and hirudin.", XP002171302, Database accession no. PREV200000412801
VAN RIJN J L M L ET AL: "KINETIC STUDIES OF PROTHROMBIN ACTIVATION EFFECT OF FACTOR V-A AND PHOSPHOLIPIDS ON THE FORMATION OF THE ENZYME-SUBSTRATE COMPLEX", BIOCHEMISTRY, vol. 23, no. 20, 1984, pages 4557 - 4564, XP002171301, ISSN: 0006-2960
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; May 2000 (2000-05-01), CALATZIS AN ET AL: "Prothrombinase-induced clotting time (PiCT): A new assay for the monitoring of heparins and hirudin.", XP002171302, Database accession no. PREV200000412801
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