Title:
HGF PRECURSOR PROTEIN MUTANT AND ACTIVATED FORM THEREOF
Document Type and Number:
WIPO Patent Application WO/2007/122975
Kind Code:
A1
Abstract:
Disclosed is an HGF precursor protein mutant which has an HGF peptide structure composed of: an HGF α-chain region or a polypeptide region having the deletion of 1 to 20 amino acid residues from the C-terminus in the HGF α-chain; an HGF β-chain region or a polypeptide region having the deletion of 1 to 20 amino acid residues from the N-terminus in the HGF β-chain; and a peptide chain X inserted between the above-mentioned two regions, wherein the peptide chain X has an amino acid sequence composed of at least two amino acid residues cleavable by a protease reaction or a chemical reaction. When the HGF precursor protein mutant is cleaved at at least one site on the peptide chain X, a protein produced by the cleavage shows an HGF activity.
Inventors:
NAKAMURA TOSHIKAZU (JP)
MATSUMOTO KUNIO (JP)
FUKUTA KAZUHIRO (JP)
ADACHI KIICHI (JP)
HAYATA DAICHIKA (JP)
MATSUMOTO KUNIO (JP)
FUKUTA KAZUHIRO (JP)
ADACHI KIICHI (JP)
HAYATA DAICHIKA (JP)
Application Number:
PCT/JP2007/057109
Publication Date:
November 01, 2007
Filing Date:
March 30, 2007
Export Citation:
Assignee:
KRINGLE PHARMA INC (JP)
UNIV OSAKA (JP)
NAKAMURA TOSHIKAZU (JP)
MATSUMOTO KUNIO (JP)
FUKUTA KAZUHIRO (JP)
ADACHI KIICHI (JP)
HAYATA DAICHIKA (JP)
UNIV OSAKA (JP)
NAKAMURA TOSHIKAZU (JP)
MATSUMOTO KUNIO (JP)
FUKUTA KAZUHIRO (JP)
ADACHI KIICHI (JP)
HAYATA DAICHIKA (JP)
International Classes:
C07K14/475; A61K38/00; A61P1/00; A61P1/16; A61P7/00; A61P11/00; A61P13/12; A61P17/00; A61P17/16; A61P25/00; A61P35/00; A61P37/06; C07K19/00; C12P21/02; C12P21/06; C12N15/09
Foreign References:
| JP2002541813A | 2002-12-10 | |||
| JP2005046030A | 2005-02-24 | |||
| JPH0418028A | 1992-01-22 | |||
| JPH0449246A | 1992-02-18 | |||
| JPH07179356A | 1995-07-18 | |||
| JPH0625010A | 1994-02-01 | |||
| JPH06340546A | 1994-12-13 | |||
| JPH06172207A | 1994-06-21 | |||
| JPH0789869A | 1995-04-04 | |||
| JPH0640934A | 1994-02-15 | |||
| JPH06503949A | 1994-05-12 | |||
| JPH0640935A | 1994-02-15 | |||
| JPH0656692A | 1994-03-01 | |||
| JPH0741429A | 1995-02-10 | |||
| JP3395181B2 | 2003-04-07 | |||
| JPH05213721A | 1993-08-24 | |||
| JPH0114696A | ||||
| JPH10191991A | 1998-07-28 | |||
| JPH03168087A | 1991-07-19 | |||
| JPS6128521A | 1986-02-08 |
Other References:
NAKAMURA T.: "Kansaibo Zoshoku Inshi (HGF) no Bunshi Cloning", EXPERIMENTAL MEDICINE, vol. 8, no. 3, 1990, pages 250 - 255, XP003012979
HUNT S.M. ET AL.: "Processing of mutated human proinsulin to mature insulin in the non-endocrine cell line, CHO", CYTOTECHNOLOGY, vol. 21, no. 3, 1996, pages 279 - 288, XP003012980
MARK M.R. ET AL.: "Expression and characterization of hepatocyte growth factor receptor-IgG fusion proteins. Effects of mutations in the potential proteolytic cleavage site on processing and ligand binding", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 267, no. 36, 1992, pages 26166 - 26171, XP002106575
S. M. N. HUNT ET AL.: "expressed a mutated human proinsulin gene with an engineered furin recognition sequence in CHO cells", CYTOTECHNOLOGY, vol. 21, 1996, pages 279 - 288
HUNT S.M. ET AL.: "Processing of mutated human proinsulin to mature insulin in the non-endocrine cell line, CHO", CYTOTECHNOLOGY, vol. 21, no. 3, 1996, pages 279 - 288, XP003012980
MARK M.R. ET AL.: "Expression and characterization of hepatocyte growth factor receptor-IgG fusion proteins. Effects of mutations in the potential proteolytic cleavage site on processing and ligand binding", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 267, no. 36, 1992, pages 26166 - 26171, XP002106575
S. M. N. HUNT ET AL.: "expressed a mutated human proinsulin gene with an engineered furin recognition sequence in CHO cells", CYTOTECHNOLOGY, vol. 21, 1996, pages 279 - 288
Attorney, Agent or Firm:
IWATANI, Ryo (3F 1-31, Dojima 2-chome,Kita-ku, Osaka-sh, Osaka 03, JP)
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