Title:
HIGH-STABILITY RECOMBINANT COLLAGEN, AND CONSTRUCTION METHOD THEREFOR AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2024/032467
Kind Code:
A1
Abstract:
Disclosed in the present invention are a high-stability recombinant collagen, and a construction method therefor and the use thereof. On the basis of an original collagen sequence, one or more mutations selected from a group consisting of avoiding an unstable GXY triplet, removing potential MMP cleavage sites, eliminating unstable chemical factors, and increasing the content of RGD in the sequence are performed. The method can be extended to expression systems other than Pichia pastoris. The recombinant collagen obtained by means of the method has physicochemical properties and biological functions similar to those of the original sequence. When used as a biological material, the recombinant collagen has high inter-batch consistency, is convenient for quality control and facilitates product stability due to having higher stability.
Inventors:
WANG LIPING (CN)
LIU XIAOHAI (CN)
QIAN CHENMING (CN)
CHENG PENGFEI (CN)
DOU RONGKUN (CN)
FAN XIAOJU (CN)
QIAN SONG (CN)
LIU XIAOHAI (CN)
QIAN CHENMING (CN)
CHENG PENGFEI (CN)
DOU RONGKUN (CN)
FAN XIAOJU (CN)
QIAN SONG (CN)
Application Number:
PCT/CN2023/111028
Publication Date:
February 15, 2024
Filing Date:
August 03, 2023
Export Citation:
Assignee:
JIANGSU TRAUTEC MEDICAL TECH CO LTD (CN)
International Classes:
C07K14/78; A61K8/65; A61Q19/00; C12N1/19; C12N15/12
Foreign References:
| CN116082493A | 2023-05-09 | |||
| US20090291474A1 | 2009-11-26 | |||
| CN103102407A | 2013-05-15 | |||
| CN111040021A | 2020-04-21 | |||
| CN113185604A | 2021-07-30 | |||
| US20060035336A1 | 2006-02-16 |
Attorney, Agent or Firm:
NANJING IPZHI INTELLECTUAL PROPERTYAGENCY CO..LTD (CN)
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